(Linear) (Six-base) MAPSORT of: KIEE0910.seq  Check: 3375  from: 1  to: 693



KIEE0910.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     693 
   Size:        358     335
 
AfeI AGC'GCT

Cuts at:      0     428     693 
   Size:        428     265
 
AleI CACnn'nnGTG

Cuts at:      0     536     693 
   Size:        536     157
 
AlwNI CAG_nnn'CTG

Cuts at:      0     118     633     693 
   Size:        118     515      60

  Fragments arranged by size: 

                 515     118      60
 
BamHI G'GATC_C

Cuts at:      0      58     693 
   Size:         58     635
 
BanII G_rGCy'C

Cuts at:      0     659     693 
   Size:        659      34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     478     693 
   Size:        478     215
 
BmtI G_CTAG'C

Cuts at:      0     248     693 
   Size:        248     445
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     499     693 
   Size:        397     102     194

  Fragments arranged by size: 

                 397     194     102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     242     693 
   Size:        242     451
 
BsaWI w'CCGG_w

Cuts at:      0     403     693 
   Size:        403     290
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     413     443     693 
   Size:        413      30     250

  Fragments arranged by size: 

                 413     250      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     651     693 
   Size:         87     564      42

  Fragments arranged by size: 

                 564      87      42
 
BsiEI CG_ry'CG

Cuts at:      0     458     693 
   Size:        458     235
 
Bsp1286I G_dGCh'C

Cuts at:      0     659     693 
   Size:        659      34
 
BsrGI T'GTAC_A

Cuts at:      0     464     693 
   Size:        464     229
 
BstYI r'GATC_y

Cuts at:      0      58     693 
   Size:         58     635
 
Bsu36I CC'TnA_GG

Cuts at:      0     188     693 
   Size:        188     505
 
BtgI C'CryG_G

Cuts at:      0     443     693 
   Size:        443     250
 
DraI TTT'AAA

Cuts at:      0     546     693 
   Size:        546     147
 
EaeI y'GGCC_r

Cuts at:      0     455     508     693 
   Size:        455      53     185

  Fragments arranged by size: 

                 455     185      53
 
EagI C'GGCC_G

Cuts at:      0     455     693 
   Size:        455     238
 
EarI CTCTTCn'nnn_

Cuts at:      0     647     693 
   Size:        647      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     397     499     693 
   Size:        358      39     102     194

  Fragments arranged by size: 

                 358     194     102      39
 
EcoRV GAT'ATC

Cuts at:      0     165     693 
   Size:        165     528
 
HaeII r_GCGC'y

Cuts at:      0     430     693 
   Size:        430     263
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     533     565     693 
   Size:        533      32     128

  Fragments arranged by size: 

                 533     128      32
 
MscI TGG'CCA

Cuts at:      0     510     693 
   Size:        510     183
 
MslI CAynn'nnrTG

Cuts at:      0     536     693 
   Size:        536     157
 
MspA1I CmG'CkG

Cuts at:      0     309     693 
   Size:        309     384
 
NheI G'CTAG_C

Cuts at:      0     244     693 
   Size:        244     449
 
PshAI GACnn'nnGTC

Cuts at:      0     478     693 
   Size:        478     215
 
PstI C_TGCA'G

Cuts at:      0     638     693 
   Size:        638      55
 
SapI GCTCTTCn'nnn_

Cuts at:      0     647     693 
   Size:        647      46
 
ScaI AGT'ACT

Cuts at:      0      17     693 
   Size:         17     676
 
SfcI C'TryA_G

Cuts at:      0      77     634     693 
   Size:         77     557      59

  Fragments arranged by size: 

                 557      77      59
 
TatI w'GTAC_w

Cuts at:      0      15     311     464     693 
   Size:         15     296     153     229

  Fragments arranged by size: 

                 296     229     153      15
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI    BamHI    BanII     BbsI     BmtI
     BpmI     BsaI    BsaWI    BsaXI     BsgI    BsiEI Bsp1286I    BsrGI
    BstYI   Bsu36I     BtgI     DraI     EaeI     EagI     EarI  Eco57MI
    EcoRV    HaeII    Hin4I     MscI     MslI   MspA1I     NheI    PshAI
     PstI     SapI     ScaI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI