Restriction map for
pF1KE0908
(Linear) (Six-base) MAPSORT of: KIEE0908.seq Check: 3786 from: 1 to: 675
KIEE0908.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 675
Size: 583 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 675
Size: 83 592
AlwNI CAG_nnn'CTG
Cuts at: 0 106 426 483 640 675
Size: 106 320 57 157 35
Fragments arranged by size:
320 157 106 57 35
ApaI G_GGCC'C
Cuts at: 0 199 675
Size: 199 476
ApaLI G'TGCA_C
Cuts at: 0 45 675
Size: 45 630
BanI G'GyrC_C
Cuts at: 0 59 675
Size: 59 616
BanII G_rGCy'C
Cuts at: 0 199 666 675
Size: 199 467 9
Fragments arranged by size:
467 199 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2 36 675
Size: 2 34 639
Fragments arranged by size:
639 34 2
BglI GCCn_nnn'nGGC
Cuts at: 0 422 431 675
Size: 422 9 244
Fragments arranged by size:
422 244 9
BlpI GC'TnA_GC
Cuts at: 0 380 675
Size: 380 295
Bme1580I G_kGCm'C
Cuts at: 0 49 199 675
Size: 49 150 476
Fragments arranged by size:
476 150 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 403 520 617 675
Size: 169 234 117 97 58
Fragments arranged by size:
234 169 117 97 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 675
Size: 598 77
BsaHI Gr'CG_yC
Cuts at: 0 446 675
Size: 446 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 492 522 675
Size: 492 30 153
Fragments arranged by size:
492 153 30
BseYI C'CCAG_C
Cuts at: 0 172 429 476 675
Size: 172 257 47 199
Fragments arranged by size:
257 199 172 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 637 675
Size: 393 244 38
Fragments arranged by size:
393 244 38
BsiHKAI G_wGCw'C
Cuts at: 0 49 56 155 675
Size: 49 7 99 520
Fragments arranged by size:
520 99 49 7
Bsp1286I G_dGCh'C
Cuts at: 0 49 56 155 199 666 675
Size: 49 7 99 44 467 9
Fragments arranged by size:
467 99 49 44 9 7
BsrDI GCAATG_nn'
Cuts at: 0 437 675
Size: 437 238
BsrFI r'CCGG_y
Cuts at: 0 608 675
Size: 608 67
BssSI C'ACGA_G
Cuts at: 0 21 675
Size: 21 654
BtsI GCAGTG_nn'
Cuts at: 0 77 374 469 675
Size: 77 297 95 206
Fragments arranged by size:
297 206 95 77
EaeI y'GGCC_r
Cuts at: 0 593 675
Size: 593 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 403 520 583 617 675
Size: 169 234 117 63 34 58
Fragments arranged by size:
234 169 117 63 58 34
EcoO109I rG'GnC_Cy
Cuts at: 0 96 103 176 196 423 675
Size: 96 7 73 20 227 252
Fragments arranged by size:
252 227 96 73 20 7
HaeII r_GCGC'y
Cuts at: 0 591 675
Size: 591 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 647 675
Size: 615 32 28
Fragments arranged by size:
615 32 28
HincII GTy'rAC
Cuts at: 0 267 675
Size: 267 408
MspA1I CmG'CkG
Cuts at: 0 483 640 675
Size: 483 157 35
Fragments arranged by size:
483 157 35
PasI CC'CwG_GG
Cuts at: 0 100 254 675
Size: 100 154 421
Fragments arranged by size:
421 154 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 106 543 675
Size: 106 437 132
Fragments arranged by size:
437 132 106
PpuMI rG'GwC_Cy
Cuts at: 0 96 176 675
Size: 96 80 499
Fragments arranged by size:
499 96 80
PspOMI G'GGCC_C
Cuts at: 0 195 675
Size: 195 480
PstI C_TGCA'G
Cuts at: 0 72 575 645 675
Size: 72 503 70 30
Fragments arranged by size:
503 72 70 30
PvuII CAG'CTG
Cuts at: 0 640 675
Size: 640 35
SanDI GG'GwC_CC
Cuts at: 0 96 675
Size: 96 579
SfcI C'TryA_G
Cuts at: 0 68 327 571 641 675
Size: 68 259 244 70 34
Fragments arranged by size:
259 244 70 68 34
SmlI C'TyrA_G
Cuts at: 0 613 675
Size: 613 62
StuI AGG'CCT
Cuts at: 0 325 675
Size: 325 350
StyI C'CwwG_G
Cuts at: 0 596 675
Size: 596 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 492 524 675
Size: 492 32 151
Fragments arranged by size:
492 151 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 484 675
Size: 267 217 191
Fragments arranged by size:
267 217 191
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI BanI BanII BcgI
BglI BlpI Bme1580I BpmI BpuEI BsaHI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BsrFI BssSI BtsI EaeI
Eco57MI EcoO109I HaeII Hin4I HincII MspA1I PasI PflMI
PpuMI PspOMI PstI PvuII SanDI SfcI SmlI StuI
StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI