(Linear) (Six-base) MAPSORT of: KIEE0903.seq  Check: 2397  from: 1  to: 504



KIEE0903.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     175     392     504 
   Size:         89      86     217     112

  Fragments arranged by size: 

                 217     112      89      86
 
ApoI r'AATT_y

Cuts at:      0     209     430     504 
   Size:        209     221      74

  Fragments arranged by size: 

                 221     209      74
 
BanI G'GyrC_C

Cuts at:      0      15     504 
   Size:         15     489
 
BbeI G_GCGC'C

Cuts at:      0      19     504 
   Size:         19     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      53     504 
   Size:         53     451
 
BglII A'GATC_T

Cuts at:      0     186     504 
   Size:        186     318
 
BmtI G_CTAG'C

Cuts at:      0     386     504 
   Size:        386     118
 
BsaHI Gr'CG_yC

Cuts at:      0      16     504 
   Size:         16     488
 
BsaWI w'CCGG_w

Cuts at:      0     145     504 
   Size:        145     359
 
BsrDI GCAATG_nn'

Cuts at:      0     232     504 
   Size:        232     272
 
BsrFI r'CCGG_y

Cuts at:      0      12     504 
   Size:         12     492
 
BstYI r'GATC_y

Cuts at:      0     186     402     462     504 
   Size:        186     216      60      42

  Fragments arranged by size: 

                 216     186      60      42
 
DraI TTT'AAA

Cuts at:      0     126     504 
   Size:        126     378
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     175     392     504 
   Size:         89      86     217     112

  Fragments arranged by size: 

                 217     112      89      86
 
EcoRI G'AATT_C

Cuts at:      0     430     504 
   Size:        430      74
 
HaeII r_GCGC'y

Cuts at:      0      19     311     504 
   Size:         19     292     193

  Fragments arranged by size: 

                 292     193      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     387     419     454     486     504 
   Size:        387      32      35      32      18

  Fragments arranged by size: 

                 387      35      32      32      18
 
HindIII A'AGCT_T

Cuts at:      0      94     504 
   Size:         94     410
 
KasI G'GCGC_C

Cuts at:      0      15     504 
   Size:         15     489
 
NaeI GCC'GGC

Cuts at:      0      14     504 
   Size:         14     490
 
NarI GG'CG_CC

Cuts at:      0      16     504 
   Size:         16     488
 
NdeI CA'TA_TG

Cuts at:      0     240     504 
   Size:        240     264
 
NgoMIV G'CCGG_C

Cuts at:      0      12     504 
   Size:         12     492
 
NheI G'CTAG_C

Cuts at:      0     382     504 
   Size:        382     122
 
ScaI AGT'ACT

Cuts at:      0     253     301     504 
   Size:        253      48     203

  Fragments arranged by size: 

                 253     203      48
 
SfoI GGC'GCC

Cuts at:      0      17     504 
   Size:         17     487
 
SgrAI Cr'CCGG_yG

Cuts at:      0      12     504 
   Size:         12     492
 
TatI w'GTAC_w

Cuts at:      0     251     299     504 
   Size:        251      48     205

  Fragments arranged by size: 

                 251     205      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     127     159     504 
   Size:        127      32     345

  Fragments arranged by size: 

                 345     127      32
 

 Enzymes that do cut:

     AcuI     ApoI     BanI     BbeI     BbsI    BglII     BmtI    BsaHI
    BsaWI    BsrDI    BsrFI    BstYI     DraI  Eco57MI    EcoRI    HaeII
    Hin4I  HindIII     KasI     NaeI     NarI     NdeI   NgoMIV     NheI
     ScaI     SfoI    SgrAI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI