(Linear) (Six-base) MAPSORT of: KIEE0901.seq  Check: 3350  from: 1  to: 402



KIEE0901.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     402 
   Size:        314      88
 
ApoI r'AATT_y

Cuts at:      0     103     402 
   Size:        103     299
 
AseI AT'TA_AT

Cuts at:      0     242     402 
   Size:        242     160
 
BfrBI ATG'CAT

Cuts at:      0     322     402 
   Size:        322      80
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     226     261     402 
   Size:        226      35     141

  Fragments arranged by size: 

                 226     141      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     402 
   Size:        314      88
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      29     402 
   Size:         29     373
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     305     402 
   Size:        273      32      97

  Fragments arranged by size: 

                 273      97      32
 
HindIII A'AGCT_T

Cuts at:      0     190     402 
   Size:        190     212
 
NsiI A_TGCA'T

Cuts at:      0     324     402 
   Size:        324      78
 
PasI CC'CwG_GG

Cuts at:      0     221     402 
   Size:        221     181
 
PfoI T'CCnGG_A

Cuts at:      0      30     402 
   Size:         30     372
 
SfcI C'TryA_G

Cuts at:      0     298     402 
   Size:        298     104
 
SspI AAT'ATT

Cuts at:      0     204     402 
   Size:        204     198
 

 Enzymes that do cut:

     AcuI     ApoI     AseI    BfrBI    CspCI  Eco57MI    EcoNI    Hin4I
  HindIII     NsiI     PasI     PfoI     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI