Restriction map for
pF1KE0802
(Linear) (Six-base) MAPSORT of: KIEE0802.seq Check: 9349 from: 1 to: 948
KIEE0802.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 878 948
Size: 878 70
AflIII A'CryG_T
Cuts at: 0 724 735 948
Size: 724 11 213
Fragments arranged by size:
724 213 11
AleI CACnn'nnGTG
Cuts at: 0 740 948
Size: 740 208
ApaLI G'TGCA_C
Cuts at: 0 787 948
Size: 787 161
ApoI r'AATT_y
Cuts at: 0 5 948
Size: 5 943
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 169 202 948
Size: 169 33 746
Fragments arranged by size:
746 169 33
BanI G'GyrC_C
Cuts at: 0 907 948
Size: 907 41
BanII G_rGCy'C
Cuts at: 0 665 948
Size: 665 283
BglI GCCn_nnn'nGGC
Cuts at: 0 205 832 948
Size: 205 627 116
Fragments arranged by size:
627 205 116
Bme1580I G_kGCm'C
Cuts at: 0 791 948
Size: 791 157
BmgBI CAC'GTC
Cuts at: 0 150 738 948
Size: 150 588 210
Fragments arranged by size:
588 210 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 716 948
Size: 88 628 232
Fragments arranged by size:
628 232 88
BsaBI GATnn'nnATC
Cuts at: 0 112 649 948
Size: 112 537 299
Fragments arranged by size:
537 299 112
BsaHI Gr'CG_yC
Cuts at: 0 502 948
Size: 502 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 948
Size: 533 415
BsiHKAI G_wGCw'C
Cuts at: 0 80 791 948
Size: 80 711 157
Fragments arranged by size:
711 157 80
BsmI GAATG_Cn'
Cuts at: 0 926 948
Size: 926 22
Bsp1286I G_dGCh'C
Cuts at: 0 80 665 791 948
Size: 80 585 126 157
Fragments arranged by size:
585 157 126 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 367 948
Size: 367 581
BstYI r'GATC_y
Cuts at: 0 899 948
Size: 899 49
BtgI C'CryG_G
Cuts at: 0 903 948
Size: 903 45
BtsI GCAGTG_nn'
Cuts at: 0 419 948
Size: 419 529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 708 743 948
Size: 708 35 205
Fragments arranged by size:
708 205 35
EaeI y'GGCC_r
Cuts at: 0 212 357 517 833 948
Size: 212 145 160 316 115
Fragments arranged by size:
316 212 160 145 115
EarI CTCTTCn'nnn_
Cuts at: 0 196 853 948
Size: 196 657 95
Fragments arranged by size:
657 196 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 716 948
Size: 88 628 232
Fragments arranged by size:
628 232 88
EcoRV GAT'ATC
Cuts at: 0 89 948
Size: 89 859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 316 948
Size: 316 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 948
Size: 435 32 481
Fragments arranged by size:
481 435 32
HincII GTy'rAC
Cuts at: 0 588 948
Size: 588 360
HindIII A'AGCT_T
Cuts at: 0 293 948
Size: 293 655
HpaI GTT'AAC
Cuts at: 0 588 948
Size: 588 360
MscI TGG'CCA
Cuts at: 0 359 519 835 948
Size: 359 160 316 113
Fragments arranged by size:
359 316 160 113
MslI CAynn'nnrTG
Cuts at: 0 740 948
Size: 740 208
MspA1I CmG'CkG
Cuts at: 0 347 948
Size: 347 601
NcoI C'CATG_G
Cuts at: 0 903 948
Size: 903 45
NspI r_CATG'y
Cuts at: 0 728 948
Size: 728 220
PasI CC'CwG_GG
Cuts at: 0 61 426 948
Size: 61 365 522
Fragments arranged by size:
522 365 61
PciI A'CATG_T
Cuts at: 0 724 948
Size: 724 224
PvuII CAG'CTG
Cuts at: 0 347 948
Size: 347 601
SfcI C'TryA_G
Cuts at: 0 245 388 782 879 948
Size: 245 143 394 97 69
Fragments arranged by size:
394 245 143 97 69
StyI C'CwwG_G
Cuts at: 0 126 795 893 903 948
Size: 126 669 98 10 45
Fragments arranged by size:
669 126 98 45 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 791 823 948
Size: 791 32 125
Fragments arranged by size:
791 125 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 948
Size: 207 741
Enzymes that do cut:
AccI AflIII AleI ApaLI ApoI BaeI BanI BanII
BglI Bme1580I BmgBI BpmI BsaBI BsaHI BsgI BsiHKAI
BsmI Bsp1286I BstXI BstYI BtgI BtsI CspCI EaeI
EarI Eco57MI EcoRV FalI Hin4I HincII HindIII HpaI
MscI MslI MspA1I NcoI NspI PasI PciI PvuII
SfcI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI