(Linear) (Six-base) MAPSORT of: KIEE0801.seq  Check: 1793  from: 1  to: 1068



KIEE0801.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    1068 
   Size:         20    1048
 
AleI CACnn'nnGTG

Cuts at:      0     967    1068 
   Size:        967     101
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112    1068 
   Size:         80      32     956

  Fragments arranged by size: 

                 956      80      32
 
ApaLI G'TGCA_C

Cuts at:      0     850    1068 
   Size:        850     218
 
ApoI r'AATT_y

Cuts at:      0     934    1068 
   Size:        934     134
 
BamHI G'GATC_C

Cuts at:      0     494    1068 
   Size:        494     574
 
BanII G_rGCy'C

Cuts at:      0      64    1068 
   Size:         64    1004
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1040    1068 
   Size:       1040      28
 
Bme1580I G_kGCm'C

Cuts at:      0     854    1068 
   Size:        854     214
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    1068 
   Size:       1047      21
 
Bpu10I CC'TnA_GC

Cuts at:      0      63    1068 
   Size:         63    1005
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     709     973    1068 
   Size:        709     264      95

  Fragments arranged by size: 

                 709     264      95
 
BsaBI GATnn'nnATC

Cuts at:      0     790     865    1068 
   Size:        790      75     203

  Fragments arranged by size: 

                 790     203      75
 
BsaHI Gr'CG_yC

Cuts at:      0     488    1068 
   Size:        488     580
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91    1068 
   Size:         61      30     977

  Fragments arranged by size: 

                 977      61      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256    1068 
   Size:        256     812
 
BseYI C'CCAG_C

Cuts at:      0     950    1068 
   Size:        950     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229    1068 
   Size:        229     839
 
BsiHKAI G_wGCw'C

Cuts at:      0     854    1068 
   Size:        854     214
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     854    1068 
   Size:         64     790     214

  Fragments arranged by size: 

                 790     214      64
 
BsrDI GCAATG_nn'

Cuts at:      0     183    1068 
   Size:        183     885
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     301    1068 
   Size:        301     767
 
BstEII G'GTnAC_C

Cuts at:      0     202    1068 
   Size:        202     866
 
BstYI r'GATC_y

Cuts at:      0     494     992    1068 
   Size:        494     498      76

  Fragments arranged by size: 

                 498     494      76
 
BtgI C'CryG_G

Cuts at:      0     305    1068 
   Size:        305     763
 
BtsI GCAGTG_nn'

Cuts at:      0     384    1068 
   Size:        384     684
 
DraI TTT'AAA

Cuts at:      0     939    1068 
   Size:        939     129
 
EaeI y'GGCC_r

Cuts at:      0     257     419    1068 
   Size:        257     162     649

  Fragments arranged by size: 

                 649     257     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20    1047    1068 
   Size:         20    1027      21

  Fragments arranged by size: 

                1027      21      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     337    1068 
   Size:        337     731
 
EcoO109I rG'GnC_Cy

Cuts at:      0     720     876    1068 
   Size:        720     156     192

  Fragments arranged by size: 

                 720     192     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     173     205    1030    1062    1068 
   Size:         61      32      80      32     825      32       6

  Fragments arranged by size: 

                 825      80      61      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0     366    1068 
   Size:        366     702
 
MscI TGG'CCA

Cuts at:      0     421    1068 
   Size:        421     647
 
MslI CAynn'nnrTG

Cuts at:      0     967    1068 
   Size:        967     101
 
PfoI T'CCnGG_A

Cuts at:      0     809    1068 
   Size:        809     259
 
PshAI GACnn'nnGTC

Cuts at:      0     733    1068 
   Size:        733     335
 
SfcI C'TryA_G

Cuts at:      0     372    1068 
   Size:        372     696
 
SmlI C'TyrA_G

Cuts at:      0     724     988    1068 
   Size:        724     264      80

  Fragments arranged by size: 

                 724     264      80
 
StyI C'CwwG_G

Cuts at:      0     451     698     924    1068 
   Size:        451     247     226     144

  Fragments arranged by size: 

                 451     247     226     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     585    1068 
   Size:        585     483
 
TatI w'GTAC_w

Cuts at:      0     236    1068 
   Size:        236     832
 

 Enzymes that do cut:

     AcuI     AleI     AloI    ApaLI     ApoI    BamHI    BanII     BbsI
 Bme1580I     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI   BstAPI   BstEII    BstYI
     BtgI     BtsI     DraI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I
   HincII     MscI     MslI     PfoI    PshAI     SfcI     SmlI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI