Restriction map for
pF1KE0799
(Linear) (Six-base) MAPSORT of: KIEE0799.seq Check: 6044 from: 1 to: 1503
KIEE0799.orf
With 182 enzymes: SgfI *
September 2, 2010 12:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1503
Size: 808 695
ApoI r'AATT_y
Cuts at: 0 109 447 507 865 1503
Size: 109 338 60 358 638
Fragments arranged by size:
638 358 338 109 60
BamHI G'GATC_C
Cuts at: 0 220 1503
Size: 220 1283
BanII G_rGCy'C
Cuts at: 0 146 823 1503
Size: 146 677 680
Fragments arranged by size:
680 677 146
BclI T'GATC_A
Cuts at: 0 758 1250 1503
Size: 758 492 253
Fragments arranged by size:
758 492 253
BfrBI ATG'CAT
Cuts at: 0 352 1503
Size: 352 1151
BglII A'GATC_T
Cuts at: 0 65 1503
Size: 65 1438
Bme1580I G_kGCm'C
Cuts at: 0 1386 1393 1503
Size: 1386 7 110
Fragments arranged by size:
1386 110 7
BmgBI CAC'GTC
Cuts at: 0 1248 1503
Size: 1248 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 1380 1441 1503
Size: 1380 61 62
Fragments arranged by size:
1380 62 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 823 1503
Size: 668 155 680
Fragments arranged by size:
680 668 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1503
Size: 1007 496
BsaAI yAC'GTr
Cuts at: 0 612 1503
Size: 612 891
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 154 584 1127 1210 1320 1503
Size: 151 3 430 543 83 110 183
Fragments arranged by size:
543 430 183 151 110 83 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 530 1234 1503
Size: 419 111 704 269
Fragments arranged by size:
704 419 269 111
BsiHKAI G_wGCw'C
Cuts at: 0 146 1503
Size: 146 1357
Bsp1286I G_dGCh'C
Cuts at: 0 146 823 1386 1393 1503
Size: 146 677 563 7 110
Fragments arranged by size:
677 563 146 110 7
BspHI T'CATG_A
Cuts at: 0 1223 1503
Size: 1223 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 391 1503
Size: 391 1112
BsrBI CCG'CTC
Cuts at: 0 975 1503
Size: 975 528
BsrDI GCAATG_nn'
Cuts at: 0 385 1184 1503
Size: 385 799 319
Fragments arranged by size:
799 385 319
BstBI TT'CG_AA
Cuts at: 0 960 1503
Size: 960 543
BstEII G'GTnAC_C
Cuts at: 0 795 1503
Size: 795 708
BstYI r'GATC_y
Cuts at: 0 65 220 416 1503
Size: 65 155 196 1087
Fragments arranged by size:
1087 196 155 65
Bsu36I CC'TnA_GG
Cuts at: 0 9 1503
Size: 9 1494
BtgI C'CryG_G
Cuts at: 0 226 876 1120 1503
Size: 226 650 244 383
Fragments arranged by size:
650 383 244 226
BtsI GCAGTG_nn'
Cuts at: 0 194 599 1503
Size: 194 405 904
Fragments arranged by size:
904 405 194
DraI TTT'AAA
Cuts at: 0 1233 1503
Size: 1233 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 28 318 1503
Size: 28 290 1185
Fragments arranged by size:
1185 290 28
EaeI y'GGCC_r
Cuts at: 0 489 1503
Size: 489 1014
EarI CTCTTCn'nnn_
Cuts at: 0 784 1419 1503
Size: 784 635 84
Fragments arranged by size:
784 635 84
EcoICRI GAG'CTC
Cuts at: 0 144 1503
Size: 144 1359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 808 823 1503
Size: 668 140 15 680
Fragments arranged by size:
680 668 140 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 683 1503
Size: 683 820
EcoO109I rG'GnC_Cy
Cuts at: 0 541 1027 1503
Size: 541 486 476
Fragments arranged by size:
541 486 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 224 1503
Size: 224 1279
FspI TGC'GCA
Cuts at: 0 1183 1503
Size: 1183 320
FspAI rTGC'GCAy
Cuts at: 0 1183 1503
Size: 1183 320
HincII GTy'rAC
Cuts at: 0 180 1503
Size: 180 1323
MfeI C'AATT_G
Cuts at: 0 437 1503
Size: 437 1066
MscI TGG'CCA
Cuts at: 0 491 1503
Size: 491 1012
NcoI C'CATG_G
Cuts at: 0 876 1503
Size: 876 627
NsiI A_TGCA'T
Cuts at: 0 354 1503
Size: 354 1149
PflMI CCAn_nnn'nTGG
Cuts at: 0 442 1503
Size: 442 1061
PfoI T'CCnGG_A
Cuts at: 0 645 919 1503
Size: 645 274 584
Fragments arranged by size:
645 584 274
PmlI CAC'GTG
Cuts at: 0 612 1503
Size: 612 891
PstI C_TGCA'G
Cuts at: 0 1343 1503
Size: 1343 160
SacI G_AGCT'C
Cuts at: 0 146 1503
Size: 146 1357
SfcI C'TryA_G
Cuts at: 0 680 1339 1503
Size: 680 659 164
Fragments arranged by size:
680 659 164
SmlI C'TyrA_G
Cuts at: 0 1022 1503
Size: 1022 481
SspI AAT'ATT
Cuts at: 0 660 1503
Size: 660 843
StyI C'CwwG_G
Cuts at: 0 502 876 993 1503
Size: 502 374 117 510
Fragments arranged by size:
510 502 374 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1347 1503
Size: 1347 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1503
Size: 551 32 920
Fragments arranged by size:
920 551 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 927 1393 1503
Size: 927 466 110
Fragments arranged by size:
927 466 110
Enzymes that do cut:
AcuI ApoI BamHI BanII BclI BfrBI BglII Bme1580I
BmgBI BmrI BpmI BpuEI BsaAI BseRI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BstBI BstEII BstYI
Bsu36I BtgI BtsI DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HincII MfeI
MscI NcoI NsiI PflMI PfoI PmlI PstI SacI
SfcI SmlI SspI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BglI BlpI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstZ17I BtgZI ClaI CspCI DraIII EagI
EciI EcoRI EcoRV FseI HaeII Hin4I HindIII HpaI
KasI KpnI MluI MmeI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI