Restriction map for
pF1KE0796
(Linear) (Six-base) MAPSORT of: KIEE0796.seq Check: 6602 from: 1 to: 1272
KIEE0796.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AfeI AGC'GCT
Cuts at: 0 718 1272
Size: 718 554
ApaI G_GGCC'C
Cuts at: 0 1224 1272
Size: 1224 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 594 627 1272
Size: 594 33 645
Fragments arranged by size:
645 594 33
BanI G'GyrC_C
Cuts at: 0 193 1272
Size: 193 1079
BanII G_rGCy'C
Cuts at: 0 1224 1258 1272
Size: 1224 34 14
Fragments arranged by size:
1224 34 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 678 1253 1272
Size: 678 575 19
Fragments arranged by size:
678 575 19
BbvCI CC'TCA_GC
Cuts at: 0 1085 1272
Size: 1085 187
Bme1580I G_kGCm'C
Cuts at: 0 257 900 1224 1272
Size: 257 643 324 48
Fragments arranged by size:
643 324 257 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 903 1272
Size: 903 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 539 891 995 1027 1272
Size: 524 15 352 104 32 245
Fragments arranged by size:
524 352 245 104 32 15
Bpu10I CC'TnA_GC
Cuts at: 0 1085 1272
Size: 1085 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 913 943 1272
Size: 277 30 606 30 329
Fragments arranged by size:
606 329 277 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 1156 1272
Size: 93 1063 116
Fragments arranged by size:
1063 116 93
BseYI C'CCAG_C
Cuts at: 0 806 1272
Size: 806 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1272
Size: 1018 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1209 1272
Size: 280 929 63
Fragments arranged by size:
929 280 63
Bsp1286I G_dGCh'C
Cuts at: 0 257 900 1224 1258 1272
Size: 257 643 324 34 14
Fragments arranged by size:
643 324 257 34 14
BsrDI GCAATG_nn'
Cuts at: 0 494 1272
Size: 494 778
BsrGI T'GTAC_A
Cuts at: 0 140 1272
Size: 140 1132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 833 1272
Size: 833 439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 939 1272
Size: 447 492 333
Fragments arranged by size:
492 447 333
Bsu36I CC'TnA_GG
Cuts at: 0 671 1272
Size: 671 601
BtgI C'CryG_G
Cuts at: 0 991 1239 1272
Size: 991 248 33
Fragments arranged by size:
991 248 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 45 1077 1272
Size: 45 1032 195
Fragments arranged by size:
1032 195 45
BtsI GCAGTG_nn'
Cuts at: 0 591 786 1272
Size: 591 195 486
Fragments arranged by size:
591 486 195
EarI CTCTTCn'nnn_
Cuts at: 0 1081 1118 1272
Size: 1081 37 154
Fragments arranged by size:
1081 154 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 539 891 995 1027 1272
Size: 524 15 352 104 32 245
Fragments arranged by size:
524 352 245 104 32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1228 1272
Size: 1228 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1221 1272
Size: 1221 51
HaeII r_GCGC'y
Cuts at: 0 720 971 1272
Size: 720 251 301
Fragments arranged by size:
720 301 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 377 409 913 945 1272
Size: 279 32 66 32 504 32 327
Fragments arranged by size:
504 327 279 66 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1180 1272
Size: 1180 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1272
Size: 241 1031
MslI CAynn'nnrTG
Cuts at: 0 425 1272
Size: 425 847
MspA1I CmG'CkG
Cuts at: 0 1084 1272
Size: 1084 188
NcoI C'CATG_G
Cuts at: 0 991 1272
Size: 991 281
PfoI T'CCnGG_A
Cuts at: 0 860 1272
Size: 860 412
PspOMI G'GGCC_C
Cuts at: 0 1220 1272
Size: 1220 52
PstI C_TGCA'G
Cuts at: 0 657 829 986 1272
Size: 657 172 157 286
Fragments arranged by size:
657 286 172 157
PvuII CAG'CTG
Cuts at: 0 1084 1272
Size: 1084 188
SapI GCTCTTCn'nnn_
Cuts at: 0 1081 1272
Size: 1081 191
SfcI C'TryA_G
Cuts at: 0 29 205 653 825 982 1272
Size: 29 176 448 172 157 290
Fragments arranged by size:
448 290 176 172 157 29
StyI C'CwwG_G
Cuts at: 0 991 1272
Size: 991 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 984 1004 1272
Size: 984 20 268
Fragments arranged by size:
984 268 20
TatI w'GTAC_w
Cuts at: 0 140 1272
Size: 140 1132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1237 1272
Size: 1237 35
XmnI GAAnn'nnTTC
Cuts at: 0 53 1272
Size: 53 1219
Enzymes that do cut:
AfeI ApaI BaeI BanI BanII BbsI BbvCI Bme1580I
BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsmBI
Bsp1286I BsrDI BsrGI BstAPI BstXI Bsu36I BtgI BtgZI
BtsI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MmeI MslI MspA1I NcoI PfoI PspOMI PstI PvuII
SapI SfcI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BbeI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI