Restriction map for
pF1KE0793
(Linear) (Six-base) MAPSORT of: KIEE0793.seq Check: 8205 from: 1 to: 936
KIEE0793.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 599 869 936
Size: 599 270 67
Fragments arranged by size:
599 270 67
AflIII A'CryG_T
Cuts at: 0 303 936
Size: 303 633
AlwNI CAG_nnn'CTG
Cuts at: 0 451 936
Size: 451 485
ApaI G_GGCC'C
Cuts at: 0 908 936
Size: 908 28
ApoI r'AATT_y
Cuts at: 0 371 610 936
Size: 371 239 326
Fragments arranged by size:
371 326 239
AvaI C'yCGr_G
Cuts at: 0 163 461 524 936
Size: 163 298 63 412
Fragments arranged by size:
412 298 163 63
BanII G_rGCy'C
Cuts at: 0 409 462 908 936
Size: 409 53 446 28
Fragments arranged by size:
446 409 53 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 550 936
Size: 550 386
BglI GCCn_nnn'nGGC
Cuts at: 0 297 936
Size: 297 639
BlpI GC'TnA_GC
Cuts at: 0 821 936
Size: 821 115
Bme1580I G_kGCm'C
Cuts at: 0 908 936
Size: 908 28
BmgBI CAC'GTC
Cuts at: 0 306 381 936
Size: 306 75 555
Fragments arranged by size:
555 306 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 936
Size: 18 918
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 423 936
Size: 423 513
BsaWI w'CCGG_w
Cuts at: 0 323 936
Size: 323 613
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 743 773 936
Size: 743 30 163
Fragments arranged by size:
743 163 30
BseYI C'CCAG_C
Cuts at: 0 5 936
Size: 5 931
BsiEI CG_ry'CG
Cuts at: 0 485 758 936
Size: 485 273 178
Fragments arranged by size:
485 273 178
BsmBI CGTCTCn'nnnn_
Cuts at: 0 312 936
Size: 312 624
Bsp1286I G_dGCh'C
Cuts at: 0 409 462 908 936
Size: 409 53 446 28
Fragments arranged by size:
446 409 53 28
BsrBI CCG'CTC
Cuts at: 0 258 402 754 936
Size: 258 144 352 182
Fragments arranged by size:
352 258 182 144
BsrFI r'CCGG_y
Cuts at: 0 646 936
Size: 646 290
BsrGI T'GTAC_A
Cuts at: 0 140 936
Size: 140 796
BtgI C'CryG_G
Cuts at: 0 518 908 936
Size: 518 390 28
Fragments arranged by size:
518 390 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 183 936
Size: 183 753
BtsI GCAGTG_nn'
Cuts at: 0 626 936
Size: 626 310
EaeI y'GGCC_r
Cuts at: 0 102 936
Size: 102 834
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 26 936
Size: 26 910
EcoO109I rG'GnC_Cy
Cuts at: 0 206 333 936
Size: 206 127 603
Fragments arranged by size:
603 206 127
EcoRI G'AATT_C
Cuts at: 0 610 936
Size: 610 326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 335 936
Size: 293 42 601
Fragments arranged by size:
601 293 42
MspA1I CmG'CkG
Cuts at: 0 829 835 877 910 936
Size: 829 6 42 33 26
Fragments arranged by size:
829 42 33 26 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 569 936
Size: 569 367
PpuMI rG'GwC_Cy
Cuts at: 0 206 333 936
Size: 206 127 603
Fragments arranged by size:
603 206 127
PshAI GACnn'nnGTC
Cuts at: 0 112 936
Size: 112 824
PspOMI G'GGCC_C
Cuts at: 0 904 936
Size: 904 32
PstI C_TGCA'G
Cuts at: 0 834 936
Size: 834 102
PvuI CG_AT'CG
Cuts at: 0 485 936
Size: 485 451
PvuII CAG'CTG
Cuts at: 0 877 936
Size: 877 59
SacII CC_GC'GG
Cuts at: 0 911 936
Size: 911 25
SanDI GG'GwC_CC
Cuts at: 0 206 333 936
Size: 206 127 603
Fragments arranged by size:
603 206 127
SfcI C'TryA_G
Cuts at: 0 275 810 830 846 918 936
Size: 275 535 20 16 72 18
Fragments arranged by size:
535 275 72 20 18 16
SmaI CCC'GGG
Cuts at: 0 463 936
Size: 463 473
StyI C'CwwG_G
Cuts at: 0 410 936
Size: 410 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 470 936
Size: 470 466
TatI w'GTAC_w
Cuts at: 0 140 936
Size: 140 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 722 743 754 775 936
Size: 722 21 11 21 161
Fragments arranged by size:
722 161 21 21 11
XmaI C'CCGG_G
Cuts at: 0 461 936
Size: 461 475
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI AvaI BanII BbsI
BglI BlpI Bme1580I BmgBI BmrI BplI BsaWI BsaXI
BseYI BsiEI BsmBI Bsp1286I BsrBI BsrFI BsrGI BtgI
BtgZI BtsI EaeI EciI EcoO109I EcoRI MmeI MspA1I
PflMI PpuMI PshAI PspOMI PstI PvuI PvuII SacII
SanDI SfcI SmaI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BsgI BsiHKAI BsiWI BsmI
BspEI BspHI BspMI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI Eco57MI EcoNI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI