Restriction map for
pF1KE0774
(Linear) (Six-base) MAPSORT of: KIEE0774.seq Check: 3480 from: 1 to: 1335
KIEE0774.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 741 1145 1335
Size: 741 404 190
Fragments arranged by size:
741 404 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 923 1335
Size: 896 27 412
Fragments arranged by size:
896 412 27
AlwNI CAG_nnn'CTG
Cuts at: 0 1021 1335
Size: 1021 314
ApaI G_GGCC'C
Cuts at: 0 1087 1335
Size: 1087 248
ApoI r'AATT_y
Cuts at: 0 532 826 1099 1335
Size: 532 294 273 236
Fragments arranged by size:
532 294 273 236
AseI AT'TA_AT
Cuts at: 0 454 662 1335
Size: 454 208 673
Fragments arranged by size:
673 454 208
BanI G'GyrC_C
Cuts at: 0 40 1335
Size: 40 1295
BanII G_rGCy'C
Cuts at: 0 1087 1120 1335
Size: 1087 33 215
Fragments arranged by size:
1087 215 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 206 1335
Size: 206 1129
BclI T'GATC_A
Cuts at: 0 1110 1335
Size: 1110 225
BfrBI ATG'CAT
Cuts at: 0 451 1335
Size: 451 884
Bme1580I G_kGCm'C
Cuts at: 0 43 1087 1335
Size: 43 1044 248
Fragments arranged by size:
1044 248 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1104 1335
Size: 1104 231
Bpu10I CC'TnA_GC
Cuts at: 0 720 1335
Size: 720 615
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1335
Size: 64 1271
BsaAI yAC'GTr
Cuts at: 0 1197 1335
Size: 1197 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 1335
Size: 19 30 1286
Fragments arranged by size:
1286 30 19
BsiHKAI G_wGCw'C
Cuts at: 0 721 1335
Size: 721 614
Bsp1286I G_dGCh'C
Cuts at: 0 43 721 1087 1120 1335
Size: 43 678 366 33 215
Fragments arranged by size:
678 366 215 43 33
BspHI T'CATG_A
Cuts at: 0 1037 1335
Size: 1037 298
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 1156 1335
Size: 885 271 179
Fragments arranged by size:
885 271 179
BstYI r'GATC_y
Cuts at: 0 118 627 1017 1053 1230 1335
Size: 118 509 390 36 177 105
Fragments arranged by size:
509 390 177 118 105 36
BstZ17I GTA'TAC
Cuts at: 0 742 1335
Size: 742 593
BtsI GCAGTG_nn'
Cuts at: 0 855 1335
Size: 855 480
EaeI y'GGCC_r
Cuts at: 0 1285 1335
Size: 1285 50
EarI CTCTTCn'nnn_
Cuts at: 0 233 419 921 1335
Size: 233 186 502 414
Fragments arranged by size:
502 414 233 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 923 1104 1335
Size: 896 27 181 231
Fragments arranged by size:
896 231 181 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 1102 1134 1299 1331 1335
Size: 1055 32 15 32 165 32 4
Fragments arranged by size:
1055 165 32 32 32 15 4
HindIII A'AGCT_T
Cuts at: 0 782 1335
Size: 782 553
MscI TGG'CCA
Cuts at: 0 1287 1335
Size: 1287 48
MslI CAynn'nnrTG
Cuts at: 0 1154 1335
Size: 1154 181
MspA1I CmG'CkG
Cuts at: 0 83 1335
Size: 83 1252
NsiI A_TGCA'T
Cuts at: 0 453 1335
Size: 453 882
NspI r_CATG'y
Cuts at: 0 685 1335
Size: 685 650
PacI TTA_AT'TAA
Cuts at: 0 662 1335
Size: 662 673
PsiI TTA'TAA
Cuts at: 0 1178 1335
Size: 1178 157
PspOMI G'GGCC_C
Cuts at: 0 1083 1335
Size: 1083 252
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 789 821 1335
Size: 789 32 514
Fragments arranged by size:
789 514 32
PstI C_TGCA'G
Cuts at: 0 381 1212 1335
Size: 381 831 123
Fragments arranged by size:
831 381 123
SapI GCTCTTCn'nnn_
Cuts at: 0 233 419 1335
Size: 233 186 916
Fragments arranged by size:
916 233 186
SfcI C'TryA_G
Cuts at: 0 377 1208 1240 1335
Size: 377 831 32 95
Fragments arranged by size:
831 377 95 32
SnaBI TAC'GTA
Cuts at: 0 1197 1335
Size: 1197 138
StyI C'CwwG_G
Cuts at: 0 413 878 1335
Size: 413 465 457
Fragments arranged by size:
465 457 413
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 1335
Size: 19 32 1284
Fragments arranged by size:
1284 32 19
XmnI GAAnn'nnTTC
Cuts at: 0 1103 1335
Size: 1103 232
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AseI BanI BanII
BbsI BclI BfrBI Bme1580I BpmI Bpu10I BsaI BsaAI
BsaXI BsiHKAI Bsp1286I BspHI BstXI BstYI BstZ17I BtsI
EaeI EarI Eco57MI Hin4I HindIII MscI MslI MspA1I
NsiI NspI PacI PsiI PspOMI PsrI PstI SapI
SfcI SnaBI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI ZraI