Restriction map for
pF1KE0769
(Linear) (Six-base) MAPSORT of: KIEE0769.seq Check: 3858 from: 1 to: 1017
KIEE0769.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 161 827 1017
Size: 161 666 190
Fragments arranged by size:
666 190 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1017
Size: 274 743
AleI CACnn'nnGTG
Cuts at: 0 448 1017
Size: 448 569
AlwNI CAG_nnn'CTG
Cuts at: 0 147 659 1011 1017
Size: 147 512 352 6
Fragments arranged by size:
512 352 147 6
ApaI G_GGCC'C
Cuts at: 0 236 944 1017
Size: 236 708 73
Fragments arranged by size:
708 236 73
AvaI C'yCGr_G
Cuts at: 0 486 536 572 1017
Size: 486 50 36 445
Fragments arranged by size:
486 445 50 36
BanI G'GyrC_C
Cuts at: 0 38 246 768 1017
Size: 38 208 522 249
Fragments arranged by size:
522 249 208 38
BanII G_rGCy'C
Cuts at: 0 88 224 236 293 944 1017
Size: 88 136 12 57 651 73
Fragments arranged by size:
651 136 88 73 57 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 774 1017
Size: 774 243
BbvCI CC'TCA_GC
Cuts at: 0 976 1017
Size: 976 41
BclI T'GATC_A
Cuts at: 0 303 1017
Size: 303 714
BglI GCCn_nnn'nGGC
Cuts at: 0 67 1017
Size: 67 950
Bme1580I G_kGCm'C
Cuts at: 0 43 213 236 251 408 666 773 944
Size: 43 170 23 15 157 258 107 171
Cuts at: 944 1017
Size: 73
Fragments arranged by size:
258 171 170 157 107 73 43 23
15
BmgBI CAC'GTC
Cuts at: 0 687 1017
Size: 687 330
BmtI G_CTAG'C
Cuts at: 0 728 1017
Size: 728 289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 862 1017
Size: 636 226 155
Fragments arranged by size:
636 226 155
Bpu10I CC'TnA_GC
Cuts at: 0 713 976 1017
Size: 713 263 41
Fragments arranged by size:
713 263 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 339 508 1017
Size: 339 169 509
Fragments arranged by size:
509 339 169
BsaWI w'CCGG_w
Cuts at: 0 785 1017
Size: 785 232
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 940 1017
Size: 217 723 77
Fragments arranged by size:
723 217 77
BseYI C'CCAG_C
Cuts at: 0 87 518 690 717 835 1017
Size: 87 431 172 27 118 182
Fragments arranged by size:
431 182 172 118 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 489 1017
Size: 133 356 528
Fragments arranged by size:
528 356 133
BsiEI CG_ry'CG
Cuts at: 0 300 1017
Size: 300 717
BsiHKAI G_wGCw'C
Cuts at: 0 818 1017
Size: 818 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 677 1017
Size: 677 340
Bsp1286I G_dGCh'C
Cuts at: 0 43 88 213 224 236 251 293 408
Size: 43 45 125 11 12 15 42 115
Cuts at: 408 666 773 818 944 1017
Size: 258 107 45 126 73
Fragments arranged by size:
258 126 125 115 107 73 45 45
43 42 15 12 11
BspEI T'CCGG_A
Cuts at: 0 785 1017
Size: 785 232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 827 1017
Size: 161 666 190
Fragments arranged by size:
666 190 161
BsrBI CCG'CTC
Cuts at: 0 167 207 485 1017
Size: 167 40 278 532
Fragments arranged by size:
532 278 167 40
BsrFI r'CCGG_y
Cuts at: 0 822 1017
Size: 822 195
BssSI C'ACGA_G
Cuts at: 0 591 1017
Size: 591 426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 758 1017
Size: 156 602 259
Fragments arranged by size:
602 259 156
BstYI r'GATC_y
Cuts at: 0 143 1017
Size: 143 874
Bsu36I CC'TnA_GG
Cuts at: 0 13 200 1017
Size: 13 187 817
Fragments arranged by size:
817 187 13
BtgI C'CryG_G
Cuts at: 0 79 498 1017
Size: 79 419 519
Fragments arranged by size:
519 419 79
BtsI GCAGTG_nn'
Cuts at: 0 378 763 1017
Size: 378 385 254
Fragments arranged by size:
385 378 254
DraIII CAC_nnn'GTG
Cuts at: 0 38 758 1017
Size: 38 720 259
Fragments arranged by size:
720 259 38
EaeI y'GGCC_r
Cuts at: 0 297 585 729 1017
Size: 297 288 144 288
Fragments arranged by size:
297 288 288 144
EagI C'GGCC_G
Cuts at: 0 297 1017
Size: 297 720
EarI CTCTTCn'nnn_
Cuts at: 0 106 889 1017
Size: 106 783 128
Fragments arranged by size:
783 128 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 636 862 1017
Size: 274 362 226 155
Fragments arranged by size:
362 274 226 155
EcoO109I rG'GnC_Cy
Cuts at: 0 232 276 491 577 656 695 941 1008
Size: 232 44 215 86 79 39 246 67
Cuts at: 1008 1017
Size: 9
Fragments arranged by size:
246 232 215 86 79 67 44 39
9
FspI TGC'GCA
Cuts at: 0 454 751 1017
Size: 454 297 266
Fragments arranged by size:
454 297 266
FspAI rTGC'GCAy
Cuts at: 0 751 1017
Size: 751 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1017
Size: 295 32 690
Fragments arranged by size:
690 295 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1017
Size: 374 643
MscI TGG'CCA
Cuts at: 0 587 1017
Size: 587 430
MslI CAynn'nnrTG
Cuts at: 0 448 1017
Size: 448 569
MspA1I CmG'CkG
Cuts at: 0 177 500 522 528 1017
Size: 177 323 22 6 489
Fragments arranged by size:
489 323 177 22 6
NaeI GCC'GGC
Cuts at: 0 824 1017
Size: 824 193
NgoMIV G'CCGG_C
Cuts at: 0 822 1017
Size: 822 195
NheI G'CTAG_C
Cuts at: 0 724 1017
Size: 724 293
PasI CC'CwG_GG
Cuts at: 0 229 1017
Size: 229 788
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1017
Size: 330 687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 866 898 1017
Size: 866 32 119
Fragments arranged by size:
866 119 32
PpuMI rG'GwC_Cy
Cuts at: 0 577 1017
Size: 577 440
PspOMI G'GGCC_C
Cuts at: 0 232 940 1017
Size: 232 708 77
Fragments arranged by size:
708 232 77
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 904 936 1017
Size: 904 32 81
Fragments arranged by size:
904 81 32
PstI C_TGCA'G
Cuts at: 0 527 1007 1017
Size: 527 480 10
Fragments arranged by size:
527 480 10
PvuII CAG'CTG
Cuts at: 0 177 522 528 1017
Size: 177 345 6 489
Fragments arranged by size:
489 345 177 6
SacII CC_GC'GG
Cuts at: 0 501 1017
Size: 501 516
SapI GCTCTTCn'nnn_
Cuts at: 0 106 1017
Size: 106 911
SexAI A'CCwGG_T
Cuts at: 0 34 776 1017
Size: 34 742 241
Fragments arranged by size:
742 241 34
SfcI C'TryA_G
Cuts at: 0 523 1003 1017
Size: 523 480 14
Fragments arranged by size:
523 480 14
SmaI CCC'GGG
Cuts at: 0 574 1017
Size: 574 443
StuI AGG'CCT
Cuts at: 0 544 1017
Size: 544 473
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1017
Size: 709 308
XmaI C'CCGG_G
Cuts at: 0 572 1017
Size: 572 445
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BclI BglI Bme1580I BmgBI BmtI BpmI
Bpu10I BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
Eco57MI EcoO109I FspI FspAI Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV NheI PasI PflMI PpiI PpuMI
PspOMI PsrI PstI PvuII SacII SapI SexAI SfcI
SmaI StuI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII
BlpI BmrI BplI BpuEI BsaAI BsaBI BsaHI BsaXI
BsiWI BsmI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacI
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI