(Linear) (Six-base) MAPSORT of: KIEE0763.seq  Check: 5208  from: 1  to: 1011



KIEE0763.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AflIII A'CryG_T

Cuts at:      0     821    1011 
   Size:        821     190
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171    1011 
   Size:        171     840
 
ApoI r'AATT_y

Cuts at:      0     390     763    1011 
   Size:        390     373     248

  Fragments arranged by size: 

                 390     373     248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299    1011 
   Size:        299     712
 
BclI T'GATC_A

Cuts at:      0     359    1011 
   Size:        359     652
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     446    1011 
   Size:        159     287     565

  Fragments arranged by size: 

                 565     287     159
 
BsaAI yAC'GTr

Cuts at:      0     378    1011 
   Size:        378     633
 
BsaHI Gr'CG_yC

Cuts at:      0     441    1011 
   Size:        441     570
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      31    1011 
   Size:          1      30     980

  Fragments arranged by size: 

                 980      30       1
 
BseYI C'CCAG_C

Cuts at:      0     283    1011 
   Size:        283     728
 
BsmI GAATG_Cn'

Cuts at:      0     845    1011 
   Size:        845     166
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      80    1011 
   Size:         80     931
 
Bsu36I CC'TnA_GG

Cuts at:      0     618    1011 
   Size:        618     393
 
BtgI C'CryG_G

Cuts at:      0     658    1011 
   Size:        658     353
 
BtsI GCAGTG_nn'

Cuts at:      0     137    1011 
   Size:        137     874
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     714     749    1011 
   Size:        714      35     262

  Fragments arranged by size: 

                 714     262      35
 
EaeI y'GGCC_r

Cuts at:      0     479    1011 
   Size:        479     532
 
EarI CTCTTCn'nnn_

Cuts at:      0     936    1011 
   Size:        936      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     280     857    1011 
   Size:        280     577     154

  Fragments arranged by size: 

                 577     280     154
 
EcoRI G'AATT_C

Cuts at:      0     763    1011 
   Size:        763     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304     598     630    1011 
   Size:        272      32     294      32     381

  Fragments arranged by size: 

                 381     294     272      32      32
 
MscI TGG'CCA

Cuts at:      0     481    1011 
   Size:        481     530
 
MslI CAynn'nnrTG

Cuts at:      0     233     872    1011 
   Size:        233     639     139

  Fragments arranged by size: 

                 639     233     139
 
MspA1I CmG'CkG

Cuts at:      0      14    1011 
   Size:         14     997
 
NspI r_CATG'y

Cuts at:      0     228     825    1011 
   Size:        228     597     186

  Fragments arranged by size: 

                 597     228     186
 
PciI A'CATG_T

Cuts at:      0     821    1011 
   Size:        821     190
 
PpuMI rG'GwC_Cy

Cuts at:      0     280     857    1011 
   Size:        280     577     154

  Fragments arranged by size: 

                 577     280     154
 
PstI C_TGCA'G

Cuts at:      0     762    1011 
   Size:        762     249
 
SanDI GG'GwC_CC

Cuts at:      0     280    1011 
   Size:        280     731
 
SfcI C'TryA_G

Cuts at:      0     758    1011 
   Size:        758     253
 
TatI w'GTAC_w

Cuts at:      0     336     488     773    1011 
   Size:        336     152     285     238

  Fragments arranged by size: 

                 336     285     238     152
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BbsI     BclI     BmrI    BsaAI    BsaHI
    BsaXI    BseYI     BsmI    BsmBI   Bsu36I     BtgI     BtsI    CspCI
     EaeI     EarI EcoO109I    EcoRI    Hin4I     MscI     MslI   MspA1I
     NspI     PciI    PpuMI     PstI    SanDI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI