Restriction map for
pF1KE0762
(Linear) (Six-base) MAPSORT of: KIEE0762.seq Check: 2479 from: 1 to: 1308
KIEE0762.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 1265 1308
Size: 1265 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1308
Size: 901 407
AflIII A'CryG_T
Cuts at: 0 161 878 1308
Size: 161 717 430
Fragments arranged by size:
717 430 161
ApoI r'AATT_y
Cuts at: 0 224 1291 1308
Size: 224 1067 17
Fragments arranged by size:
1067 224 17
AvaI C'yCGr_G
Cuts at: 0 356 850 1308
Size: 356 494 458
Fragments arranged by size:
494 458 356
BamHI G'GATC_C
Cuts at: 0 1017 1308
Size: 1017 291
BanI G'GyrC_C
Cuts at: 0 799 981 1308
Size: 799 182 327
Fragments arranged by size:
799 327 182
BanII G_rGCy'C
Cuts at: 0 473 890 1308
Size: 473 417 418
Fragments arranged by size:
473 418 417
BfrBI ATG'CAT
Cuts at: 0 561 1308
Size: 561 747
BglII A'GATC_T
Cuts at: 0 585 1308
Size: 585 723
Bme1580I G_kGCm'C
Cuts at: 0 859 1308
Size: 859 449
BmrI ACTGGGnnnn_n'
Cuts at: 0 1204 1308
Size: 1204 104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1151 1308
Size: 1151 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1308
Size: 351 957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1308
Size: 1033 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 701 1235 1308
Size: 415 286 534 73
Fragments arranged by size:
534 415 286 73
BsaAI yAC'GTr
Cuts at: 0 753 879 1308
Size: 753 126 429
Fragments arranged by size:
753 429 126
BsaHI Gr'CG_yC
Cuts at: 0 136 1308
Size: 136 1172
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 689 923 1308
Size: 127 562 234 385
Fragments arranged by size:
562 385 234 127
BseYI C'CCAG_C
Cuts at: 0 198 1308
Size: 198 1110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 421 1308
Size: 367 54 887
Fragments arranged by size:
887 367 54
BsiHKAI G_wGCw'C
Cuts at: 0 473 737 1308
Size: 473 264 571
Fragments arranged by size:
571 473 264
Bsp1286I G_dGCh'C
Cuts at: 0 473 737 859 890 1308
Size: 473 264 122 31 418
Fragments arranged by size:
473 418 264 122 31
BspHI T'CATG_A
Cuts at: 0 956 1214 1308
Size: 956 258 94
Fragments arranged by size:
956 258 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 981 1308
Size: 981 327
BstBI TT'CG_AA
Cuts at: 0 1113 1308
Size: 1113 195
BstYI r'GATC_y
Cuts at: 0 308 585 923 1017 1308
Size: 308 277 338 94 291
Fragments arranged by size:
338 308 291 277 94
BtgI C'CryG_G
Cuts at: 0 824 929 1308
Size: 824 105 379
Fragments arranged by size:
824 379 105
BtsI GCAGTG_nn'
Cuts at: 0 14 1308
Size: 14 1294
DraIII CAC_nnn'GTG
Cuts at: 0 444 1308
Size: 444 864
EaeI y'GGCC_r
Cuts at: 0 299 827 1308
Size: 299 528 481
Fragments arranged by size:
528 481 299
EarI CTCTTCn'nnn_
Cuts at: 0 478 727 1097 1308
Size: 478 249 370 211
Fragments arranged by size:
478 370 249 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 391 1308
Size: 391 917
EcoICRI GAG'CTC
Cuts at: 0 471 1308
Size: 471 837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 901 1308
Size: 351 550 407
Fragments arranged by size:
550 407 351
EcoO109I rG'GnC_Cy
Cuts at: 0 1256 1308
Size: 1256 52
EcoRI G'AATT_C
Cuts at: 0 224 1308
Size: 224 1084
EcoRV GAT'ATC
Cuts at: 0 759 1308
Size: 759 549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 283 456 543 1308
Size: 283 173 87 765
Fragments arranged by size:
765 283 173 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 221 253 412 444 1308
Size: 221 32 159 32 864
Fragments arranged by size:
864 221 159 32 32
HindIII A'AGCT_T
Cuts at: 0 265 1308
Size: 265 1043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1035 1308
Size: 1013 22 273
Fragments arranged by size:
1013 273 22
MscI TGG'CCA
Cuts at: 0 301 829 1308
Size: 301 528 479
Fragments arranged by size:
528 479 301
MslI CAynn'nnrTG
Cuts at: 0 560 751 1308
Size: 560 191 557
Fragments arranged by size:
560 557 191
MspA1I CmG'CkG
Cuts at: 0 132 1308
Size: 132 1176
NsiI A_TGCA'T
Cuts at: 0 563 1308
Size: 563 745
NspI r_CATG'y
Cuts at: 0 165 561 1308
Size: 165 396 747
Fragments arranged by size:
747 396 165
PciI A'CATG_T
Cuts at: 0 161 1308
Size: 161 1147
PfoI T'CCnGG_A
Cuts at: 0 311 725 1308
Size: 311 414 583
Fragments arranged by size:
583 414 311
PmlI CAC'GTG
Cuts at: 0 879 1308
Size: 879 429
PpuMI rG'GwC_Cy
Cuts at: 0 1256 1308
Size: 1256 52
PspXI vC'TCGA_Gb
Cuts at: 0 850 1308
Size: 850 458
PstI C_TGCA'G
Cuts at: 0 9 1308
Size: 9 1299
PvuII CAG'CTG
Cuts at: 0 132 1308
Size: 132 1176
SacI G_AGCT'C
Cuts at: 0 473 1308
Size: 473 835
SanDI GG'GwC_CC
Cuts at: 0 1256 1308
Size: 1256 52
SapI GCTCTTCn'nnn_
Cuts at: 0 478 1097 1308
Size: 478 619 211
Fragments arranged by size:
619 478 211
SfcI C'TryA_G
Cuts at: 0 5 1158 1266 1308
Size: 5 1153 108 42
Fragments arranged by size:
1153 108 42 5
SmlI C'TyrA_G
Cuts at: 0 850 1048 1308
Size: 850 198 260
Fragments arranged by size:
850 260 198
StyI C'CwwG_G
Cuts at: 0 767 1122 1308
Size: 767 355 186
Fragments arranged by size:
767 355 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 1308
Size: 106 1202
TatI w'GTAC_w
Cuts at: 0 782 1308
Size: 782 526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 294 309 438 1308
Size: 265 29 15 129 870
Fragments arranged by size:
870 265 129 29 15
XhoI C'TCGA_G
Cuts at: 0 850 1308
Size: 850 458
XmnI GAAnn'nnTTC
Cuts at: 0 685 1308
Size: 685 623
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BamHI BanI BanII
BfrBI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BstAPI BstBI BstYI BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HindIII MmeI MscI MslI MspA1I NsiI NspI PciI
PfoI PmlI PpuMI PspXI PstI PvuII SacI SanDI
SapI SfcI SmlI StyI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmtI Bpu10I BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoNI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XmaI ZraI