Restriction map for
pF1KE0754
(Linear) (Six-base) MAPSORT of: KIEE0754.seq Check: 4534 from: 1 to: 1290
KIEE0754.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 622 1290
Size: 162 460 668
Fragments arranged by size:
668 460 162
AfeI AGC'GCT
Cuts at: 0 372 1290
Size: 372 918
AflIII A'CryG_T
Cuts at: 0 583 1290
Size: 583 707
AgeI A'CCGG_T
Cuts at: 0 157 1290
Size: 157 1133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 1290
Size: 320 970
AlwNI CAG_nnn'CTG
Cuts at: 0 1018 1290
Size: 1018 272
ApaI G_GGCC'C
Cuts at: 0 795 1290
Size: 795 495
ApoI r'AATT_y
Cuts at: 0 401 432 512 570 707 1099 1113 1290
Size: 401 31 80 58 137 392 14 177
Fragments arranged by size:
401 392 177 137 80 58 31 14
AseI AT'TA_AT
Cuts at: 0 731 1290
Size: 731 559
BanI G'GyrC_C
Cuts at: 0 154 1290
Size: 154 1136
BanII G_rGCy'C
Cuts at: 0 194 795 1290
Size: 194 601 495
Fragments arranged by size:
601 495 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 491 1290
Size: 98 393 799
Fragments arranged by size:
799 393 98
BclI T'GATC_A
Cuts at: 0 717 1290
Size: 717 573
Bme1580I G_kGCm'C
Cuts at: 0 795 1290
Size: 795 495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 844 1290
Size: 844 446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1290
Size: 208 1082
Bpu10I CC'TnA_GC
Cuts at: 0 1148 1290
Size: 1148 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 1290
Size: 832 458
BsaAI yAC'GTr
Cuts at: 0 213 1290
Size: 213 1077
BsaHI Gr'CG_yC
Cuts at: 0 169 1263 1290
Size: 169 1094 27
Fragments arranged by size:
1094 169 27
BsaWI w'CCGG_w
Cuts at: 0 157 1290
Size: 157 1133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 769 1290
Size: 271 498 521
Fragments arranged by size:
521 498 271
BsiEI CG_ry'CG
Cuts at: 0 162 1290
Size: 162 1128
BsiHKAI G_wGCw'C
Cuts at: 0 1001 1290
Size: 1001 289
Bsp1286I G_dGCh'C
Cuts at: 0 194 795 1001 1290
Size: 194 601 206 289
Fragments arranged by size:
601 289 206 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 312 1290
Size: 312 978
BsrFI r'CCGG_y
Cuts at: 0 157 1290
Size: 157 1133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 687 1290
Size: 687 603
DraI TTT'AAA
Cuts at: 0 615 1104 1290
Size: 615 489 186
Fragments arranged by size:
615 489 186
DrdI GACnn_nn'nnGTC
Cuts at: 0 493 1290
Size: 493 797
EaeI y'GGCC_r
Cuts at: 0 941 1290
Size: 941 349
EarI CTCTTCn'nnn_
Cuts at: 0 22 181 527 1290
Size: 22 159 346 763
Fragments arranged by size:
763 346 159 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1290
Size: 74 1216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 208 622 1290
Size: 162 46 414 668
Fragments arranged by size:
668 414 162 46
EcoO109I rG'GnC_Cy
Cuts at: 0 791 1290
Size: 791 499
HaeII r_GCGC'y
Cuts at: 0 374 1290
Size: 374 916
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 829 844 849 861 876 1290
Size: 817 12 15 5 12 15 414
Fragments arranged by size:
817 414 15 15 12 12 5
HindIII A'AGCT_T
Cuts at: 0 1197 1290
Size: 1197 93
MslI CAynn'nnrTG
Cuts at: 0 943 1290
Size: 943 347
NspI r_CATG'y
Cuts at: 0 587 1290
Size: 587 703
PciI A'CATG_T
Cuts at: 0 583 1290
Size: 583 707
PfoI T'CCnGG_A
Cuts at: 0 44 1290
Size: 44 1246
PsiI TTA'TAA
Cuts at: 0 887 1290
Size: 887 403
PspOMI G'GGCC_C
Cuts at: 0 791 1290
Size: 791 499
PstI C_TGCA'G
Cuts at: 0 1182 1290
Size: 1182 108
SexAI A'CCwGG_T
Cuts at: 0 441 1290
Size: 441 849
SfcI C'TryA_G
Cuts at: 0 96 1178 1290
Size: 96 1082 112
Fragments arranged by size:
1082 112 96
SspI AAT'ATT
Cuts at: 0 729 1290
Size: 729 561
StyI C'CwwG_G
Cuts at: 0 5 1290
Size: 5 1285
TatI w'GTAC_w
Cuts at: 0 268 1290
Size: 268 1022
XbaI T'CTAG_A
Cuts at: 0 847 1290
Size: 847 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 148 802 1290
Size: 148 654 488
Fragments arranged by size:
654 488 148
XmnI GAAnn'nnTTC
Cuts at: 0 401 1290
Size: 401 889
Enzymes that do cut:
AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApoI
AseI BanI BanII BbsI BclI Bme1580I BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BstXI DraI DrdI EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I HindIII MslI NspI
PciI PfoI PsiI PspOMI PstI SexAI SfcI SspI
StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AleI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaBI BsaXI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XhoI XmaI ZraI