Restriction map for
pF1KE0750
(Linear) (Six-base) MAPSORT of: KIEE0750.seq Check: 6862 from: 1 to: 1278
KIEE0750.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 286 384 1278
Size: 245 41 98 894
Fragments arranged by size:
894 245 98 41
AflII C'TTAA_G
Cuts at: 0 418 1278
Size: 418 860
AflIII A'CryG_T
Cuts at: 0 999 1278
Size: 999 279
AgeI A'CCGG_T
Cuts at: 0 95 1278
Size: 95 1183
AhdI GACnn_n'nnGTC
Cuts at: 0 96 1278
Size: 96 1182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 508 1278
Size: 508 770
ApaLI G'TGCA_C
Cuts at: 0 493 1278
Size: 493 785
ApoI r'AATT_y
Cuts at: 0 102 529 587 697 894 923 1175 1278
Size: 102 427 58 110 197 29 252 103
Fragments arranged by size:
427 252 197 110 103 102 58 29
BanII G_rGCy'C
Cuts at: 0 438 1278
Size: 438 840
BbsI GAAGACnn'nnnn_
Cuts at: 0 390 543 606 1225 1278
Size: 390 153 63 619 53
Fragments arranged by size:
619 390 153 63 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 332 1278
Size: 332 946
BglII A'GATC_T
Cuts at: 0 1147 1278
Size: 1147 131
Bme1580I G_kGCm'C
Cuts at: 0 497 1278
Size: 497 781
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 1278
Size: 344 934
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 706 1278
Size: 706 572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 548 1121 1278
Size: 232 316 573 157
Fragments arranged by size:
573 316 232 157
BsaWI w'CCGG_w
Cuts at: 0 95 1278
Size: 95 1183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 546 576 1278
Size: 546 30 702
Fragments arranged by size:
702 546 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 1278
Size: 172 1106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1278
Size: 812 466
BsiHKAI G_wGCw'C
Cuts at: 0 438 497 1278
Size: 438 59 781
Fragments arranged by size:
781 438 59
Bsp1286I G_dGCh'C
Cuts at: 0 438 497 1278
Size: 438 59 781
Fragments arranged by size:
781 438 59
BsrFI r'CCGG_y
Cuts at: 0 95 1278
Size: 95 1183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 161 1278
Size: 161 1117
BstYI r'GATC_y
Cuts at: 0 883 1147 1278
Size: 883 264 131
Fragments arranged by size:
883 264 131
BtgI C'CryG_G
Cuts at: 0 21 1278
Size: 21 1257
BtsI GCAGTG_nn'
Cuts at: 0 751 1278
Size: 751 527
EaeI y'GGCC_r
Cuts at: 0 1047 1278
Size: 1047 231
EcoICRI GAG'CTC
Cuts at: 0 436 1278
Size: 436 842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 245 286 384 548 1121 1278
Size: 232 13 41 98 164 573 157
Fragments arranged by size:
573 232 164 157 98 41 13
EcoRI G'AATT_C
Cuts at: 0 1175 1278
Size: 1175 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 359 391 1278
Size: 82 32 245 32 887
Fragments arranged by size:
887 245 82 32 32
MscI TGG'CCA
Cuts at: 0 1049 1278
Size: 1049 229
MslI CAynn'nnrTG
Cuts at: 0 490 948 1278
Size: 490 458 330
Fragments arranged by size:
490 458 330
NcoI C'CATG_G
Cuts at: 0 21 1278
Size: 21 1257
NspI r_CATG'y
Cuts at: 0 835 1003 1278
Size: 835 168 275
Fragments arranged by size:
835 275 168
PasI CC'CwG_GG
Cuts at: 0 35 1278
Size: 35 1243
PciI A'CATG_T
Cuts at: 0 999 1278
Size: 999 279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1278
Size: 217 32 1029
Fragments arranged by size:
1029 217 32
SacI G_AGCT'C
Cuts at: 0 438 1278
Size: 438 840
SfcI C'TryA_G
Cuts at: 0 195 360 1278
Size: 195 165 918
Fragments arranged by size:
918 195 165
SmlI C'TyrA_G
Cuts at: 0 418 1278
Size: 418 860
SspI AAT'ATT
Cuts at: 0 329 1278
Size: 329 949
StuI AGG'CCT
Cuts at: 0 569 1278
Size: 569 709
StyI C'CwwG_G
Cuts at: 0 21 1278
Size: 21 1257
TatI w'GTAC_w
Cuts at: 0 1064 1278
Size: 1064 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 363 395 546 578 1278
Size: 363 32 151 32 700
Fragments arranged by size:
700 363 151 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 802 970 1138 1222 1278
Size: 802 168 168 84 56
Fragments arranged by size:
802 168 168 84 56
Enzymes that do cut:
AcuI AflII AflIII AgeI AhdI AlfI ApaLI ApoI
BanII BbsI BciVI BglII Bme1580I BmrI BplI BpmI
BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrFI BstXI
BstYI BtgI BtsI EaeI EcoICRI Eco57MI EcoRI Hin4I
MscI MslI NcoI NspI PasI PciI PpiI SacI
SfcI SmlI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AleI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI
BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI