Restriction map for
pF1KE0749
(Linear) (Six-base) MAPSORT of: KIEE0749.seq Check: 706 from: 1 to: 978
KIEE0749.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 978
Size: 447 531
AccI GT'mk_AC
Cuts at: 0 161 599 978
Size: 161 438 379
Fragments arranged by size:
438 379 161
AleI CACnn'nnGTG
Cuts at: 0 622 978
Size: 622 356
AlwNI CAG_nnn'CTG
Cuts at: 0 462 804 978
Size: 462 342 174
Fragments arranged by size:
462 342 174
ApaI G_GGCC'C
Cuts at: 0 44 978
Size: 44 934
ApaLI G'TGCA_C
Cuts at: 0 499 924 978
Size: 499 425 54
Fragments arranged by size:
499 425 54
AscI GG'CGCG_CC
Cuts at: 0 439 978
Size: 439 539
AvaI C'yCGr_G
Cuts at: 0 376 620 978
Size: 376 244 358
Fragments arranged by size:
376 358 244
BamHI G'GATC_C
Cuts at: 0 644 978
Size: 644 334
BanI G'GyrC_C
Cuts at: 0 418 616 815 978
Size: 418 198 199 163
Fragments arranged by size:
418 199 198 163
BanII G_rGCy'C
Cuts at: 0 44 191 554 978
Size: 44 147 363 424
Fragments arranged by size:
424 363 147 44
BbeI G_GCGC'C
Cuts at: 0 422 978
Size: 422 556
BglI GCCn_nnn'nGGC
Cuts at: 0 248 978
Size: 248 730
Bme1580I G_kGCm'C
Cuts at: 0 44 263 503 928 978
Size: 44 219 240 425 50
Fragments arranged by size:
425 240 219 50 44
BmgBI CAC'GTC
Cuts at: 0 945 959 978
Size: 945 14 19
Fragments arranged by size:
945 19 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 803 978
Size: 803 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 388 773 978
Size: 286 102 385 205
Fragments arranged by size:
385 286 205 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 571 978
Size: 538 33 407
Fragments arranged by size:
538 407 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 789 978
Size: 789 189
BsaAI yAC'GTr
Cuts at: 0 210 978
Size: 210 768
BsaHI Gr'CG_yC
Cuts at: 0 314 419 896 978
Size: 314 105 477 82
Fragments arranged by size:
477 314 105 82
BsaWI w'CCGG_w
Cuts at: 0 544 773 809 978
Size: 544 229 36 169
Fragments arranged by size:
544 229 169 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 978
Size: 526 30 422
Fragments arranged by size:
526 422 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 760 792 978
Size: 760 32 186
Fragments arranged by size:
760 186 32
BseYI C'CCAG_C
Cuts at: 0 135 690 879 978
Size: 135 555 189 99
Fragments arranged by size:
555 189 135 99
BsiEI CG_ry'CG
Cuts at: 0 257 844 978
Size: 257 587 134
Fragments arranged by size:
587 257 134
BsiHKAI G_wGCw'C
Cuts at: 0 503 554 928 978
Size: 503 51 374 50
Fragments arranged by size:
503 374 51 50
Bsp1286I G_dGCh'C
Cuts at: 0 44 191 263 503 554 928 978
Size: 44 147 72 240 51 374 50
Fragments arranged by size:
374 240 147 72 51 50 44
BspEI T'CCGG_A
Cuts at: 0 544 773 978
Size: 544 229 205
Fragments arranged by size:
544 229 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 545 923 978
Size: 447 98 378 55
Fragments arranged by size:
447 378 98 55
BsrBI CCG'CTC
Cuts at: 0 222 342 372 840 978
Size: 222 120 30 468 138
Fragments arranged by size:
468 222 138 120 30
BsrFI r'CCGG_y
Cuts at: 0 381 978
Size: 381 597
BsrGI T'GTAC_A
Cuts at: 0 626 978
Size: 626 352
BssHII G'CGCG_C
Cuts at: 0 305 307 439 978
Size: 305 2 132 539
Fragments arranged by size:
539 305 132 2
BstEII G'GTnAC_C
Cuts at: 0 225 978
Size: 225 753
BstYI r'GATC_y
Cuts at: 0 71 644 749 978
Size: 71 573 105 229
Fragments arranged by size:
573 229 105 71
BtgI C'CryG_G
Cuts at: 0 663 978
Size: 663 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 978
Size: 162 816
BtsI GCAGTG_nn'
Cuts at: 0 137 477 978
Size: 137 340 501
Fragments arranged by size:
501 340 137
DraIII CAC_nnn'GTG
Cuts at: 0 213 683 978
Size: 213 470 295
Fragments arranged by size:
470 295 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 895 978
Size: 895 83
EaeI y'GGCC_r
Cuts at: 0 464 674 841 947 978
Size: 464 210 167 106 31
Fragments arranged by size:
464 210 167 106 31
EagI C'GGCC_G
Cuts at: 0 841 978
Size: 841 137
EarI CTCTTCn'nnn_
Cuts at: 0 400 775 978
Size: 400 375 203
Fragments arranged by size:
400 375 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 310 978
Size: 106 204 668
Fragments arranged by size:
668 204 106
EcoICRI GAG'CTC
Cuts at: 0 552 978
Size: 552 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 388 773 978
Size: 286 102 385 205
Fragments arranged by size:
385 286 205 102
EcoO109I rG'GnC_Cy
Cuts at: 0 390 978
Size: 390 588
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 978
Size: 120 858
FspI TGC'GCA
Cuts at: 0 283 978
Size: 283 695
HaeII r_GCGC'y
Cuts at: 0 422 672 978
Size: 422 250 306
Fragments arranged by size:
422 306 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 829 861 978
Size: 526 32 271 32 117
Fragments arranged by size:
526 271 117 32 32
KasI G'GCGC_C
Cuts at: 0 418 978
Size: 418 560
MscI TGG'CCA
Cuts at: 0 949 978
Size: 949 29
MslI CAynn'nnrTG
Cuts at: 0 622 978
Size: 622 356
MspA1I CmG'CkG
Cuts at: 0 435 488 804 978
Size: 435 53 316 174
Fragments arranged by size:
435 316 174 53
NaeI GCC'GGC
Cuts at: 0 383 978
Size: 383 595
NarI GG'CG_CC
Cuts at: 0 419 978
Size: 419 559
NgoMIV G'CCGG_C
Cuts at: 0 381 978
Size: 381 597
PflMI CCAn_nnn'nTGG
Cuts at: 0 683 978
Size: 683 295
PfoI T'CCnGG_A
Cuts at: 0 899 978
Size: 899 79
PmlI CAC'GTG
Cuts at: 0 210 978
Size: 210 768
PpuMI rG'GwC_Cy
Cuts at: 0 390 978
Size: 390 588
PspOMI G'GGCC_C
Cuts at: 0 40 978
Size: 40 938
PstI C_TGCA'G
Cuts at: 0 80 978
Size: 80 898
PvuII CAG'CTG
Cuts at: 0 488 804 978
Size: 488 316 174
Fragments arranged by size:
488 316 174
SacI G_AGCT'C
Cuts at: 0 554 978
Size: 554 424
SapI GCTCTTCn'nnn_
Cuts at: 0 775 978
Size: 775 203
SexAI A'CCwGG_T
Cuts at: 0 119 978
Size: 119 859
SfcI C'TryA_G
Cuts at: 0 76 978
Size: 76 902
SfoI GGC'GCC
Cuts at: 0 420 978
Size: 420 558
SmlI C'TyrA_G
Cuts at: 0 553 586 978
Size: 553 33 392
Fragments arranged by size:
553 392 33
StyI C'CwwG_G
Cuts at: 0 86 978
Size: 86 892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 978
Size: 244 734
TatI w'GTAC_w
Cuts at: 0 626 978
Size: 626 352
Tth111I GACn'n_nGTC
Cuts at: 0 595 978
Size: 595 383
Enzymes that do cut:
AarI AccI AleI AlwNI ApaI ApaLI AscI AvaI
BamHI BanI BanII BbeI BglI Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstEII BstYI BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI FspI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NgoMIV PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SfoI SmlI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI Bpu10I BsaBI BsgI BsiWI BsmI BsmBI
BspHI BsrDI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI EcoNI EcoRI EcoRV FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI