Restriction map for pF1KE0741
 (Linear) (Six-base) MAPSORT of: KIEE0741.seq  Check: 2520  from: 1  to: 945



KIEE0741.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     945 
   Size:         28     917
 
AleI CACnn'nnGTG

Cuts at:      0     152     945 
   Size:        152     793
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     170     945 
   Size:        170     775
 
ApoI r'AATT_y

Cuts at:      0     776     841     892     945 
   Size:        776      65      51      53

  Fragments arranged by size: 

                 776      65      53      51
 
AvaI C'yCGr_G

Cuts at:      0     104     945 
   Size:        104     841
 
BanII G_rGCy'C

Cuts at:      0     111     197     945 
   Size:        111      86     748

  Fragments arranged by size: 

                 748     111      86
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120     945 
   Size:        120     825
 
BbvCI CC'TCA_GC

Cuts at:      0     616     945 
   Size:        616     329
 
BglII A'GATC_T

Cuts at:      0     239     945 
   Size:        239     706
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     195     945 
   Size:        195     750
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     945 
   Size:        165     780
 
Bpu10I CC'TnA_GC

Cuts at:      0     616     945 
   Size:        616     329
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     945 
   Size:        781     164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37     388     945 
   Size:         37     351     557

  Fragments arranged by size: 

                 557     351      37
 
BsaHI Gr'CG_yC

Cuts at:      0     519     945 
   Size:        519     426
 
BsiHKAI G_wGCw'C

Cuts at:      0     197     945 
   Size:        197     748
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     197     945 
   Size:        111      86     748

  Fragments arranged by size: 

                 748     111      86
 
BsrGI T'GTAC_A

Cuts at:      0     767     945 
   Size:        767     178
 
BstYI r'GATC_y

Cuts at:      0     239     945 
   Size:        239     706
 
BtgI C'CryG_G

Cuts at:      0     158     701     945 
   Size:        158     543     244

  Fragments arranged by size: 

                 543     244     158
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     789     824     945 
   Size:        789      35     121

  Fragments arranged by size: 

                 789     121      35
 
DraI TTT'AAA

Cuts at:      0     257     945 
   Size:        257     688
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     895     945 
   Size:        895      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     930     945 
   Size:        930      15
 
EcoICRI GAG'CTC

Cuts at:      0     195     945 
   Size:        195     750
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     165     945 
   Size:         28     137     780

  Fragments arranged by size: 

                 780     137      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     691     945 
   Size:        691     254
 
EcoRI G'AATT_C

Cuts at:      0     776     945 
   Size:        776     169
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     604     636     945 
   Size:        604      32     309

  Fragments arranged by size: 

                 604     309      32
 
MfeI C'AATT_G

Cuts at:      0     737     945 
   Size:        737     208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     945 
   Size:        215     730
 
MslI CAynn'nnrTG

Cuts at:      0     152     527     802     945 
   Size:        152     375     275     143

  Fragments arranged by size: 

                 375     275     152     143
 
MspA1I CmG'CkG

Cuts at:      0     141     945 
   Size:        141     804
 
PfoI T'CCnGG_A

Cuts at:      0     188     945 
   Size:        188     757
 
PpuMI rG'GwC_Cy

Cuts at:      0     691     945 
   Size:        691     254
 
PsiI TTA'TAA

Cuts at:      0     421     945 
   Size:        421     524
 
PvuII CAG'CTG

Cuts at:      0     141     945 
   Size:        141     804
 
SacI G_AGCT'C

Cuts at:      0     197     945 
   Size:        197     748
 
SmaI CCC'GGG

Cuts at:      0     106     945 
   Size:        106     839
 
SmlI C'TyrA_G

Cuts at:      0     760     945 
   Size:        760     185
 
SspI AAT'ATT

Cuts at:      0     507     945 
   Size:        507     438
 
StyI C'CwwG_G

Cuts at:      0      38     945 
   Size:         38     907
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     503     945 
   Size:        503     442
 
TatI w'GTAC_w

Cuts at:      0     767     945 
   Size:        767     178
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     165     945 
   Size:        165     780
 
XmaI C'CCGG_G

Cuts at:      0     104     945 
   Size:        104     841
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApoI     AvaI    BanII     BbsI    BbvCI
    BglII     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI  BsiHKAI
 Bsp1286I    BsrGI    BstYI     BtgI    CspCI     DraI     DrdI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I     MfeI     MmeI     MslI
   MspA1I     PfoI    PpuMI     PsiI    PvuII     SacI     SmaI     SmlI
     SspI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI   DraIII     EaeI     EagI     EciI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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