(Linear) (Six-base) MAPSORT of: KIEE0741.seq Check: 2520 from: 1 to: 945 KIEE0741.orf With 182 enzymes: SgfI * October 7, 2009 14:27 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 945 Size: 28 917 AleI CACnn'nnGTG Cuts at: 0 152 945 Size: 152 793 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 170 945 Size: 170 775 ApoI r'AATT_y Cuts at: 0 776 841 892 945 Size: 776 65 51 53 Fragments arranged by size: 776 65 53 51 AvaI C'yCGr_G Cuts at: 0 104 945 Size: 104 841 BanII G_rGCy'C Cuts at: 0 111 197 945 Size: 111 86 748 Fragments arranged by size: 748 111 86 BbsI GAAGACnn'nnnn_ Cuts at: 0 120 945 Size: 120 825 BbvCI CC'TCA_GC Cuts at: 0 616 945 Size: 616 329 BglII A'GATC_T Cuts at: 0 239 945 Size: 239 706 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 195 945 Size: 195 750 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 165 945 Size: 165 780 Bpu10I CC'TnA_GC Cuts at: 0 616 945 Size: 616 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 945 Size: 781 164 BsaI GGTCTCn'nnnn_ Cuts at: 0 37 388 945 Size: 37 351 557 Fragments arranged by size: 557 351 37 BsaHI Gr'CG_yC Cuts at: 0 519 945 Size: 519 426 BsiHKAI G_wGCw'C Cuts at: 0 197 945 Size: 197 748 Bsp1286I G_dGCh'C Cuts at: 0 111 197 945 Size: 111 86 748 Fragments arranged by size: 748 111 86 BsrGI T'GTAC_A Cuts at: 0 767 945 Size: 767 178 BstYI r'GATC_y Cuts at: 0 239 945 Size: 239 706 BtgI C'CryG_G Cuts at: 0 158 701 945 Size: 158 543 244 Fragments arranged by size: 543 244 158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 789 824 945 Size: 789 35 121 Fragments arranged by size: 789 121 35 DraI TTT'AAA Cuts at: 0 257 945 Size: 257 688 DrdI GACnn_nn'nnGTC Cuts at: 0 895 945 Size: 895 50 EarI CTCTTCn'nnn_ Cuts at: 0 930 945 Size: 930 15 EcoICRI GAG'CTC Cuts at: 0 195 945 Size: 195 750 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 165 945 Size: 28 137 780 Fragments arranged by size: 780 137 28 EcoO109I rG'GnC_Cy Cuts at: 0 691 945 Size: 691 254 EcoRI G'AATT_C Cuts at: 0 776 945 Size: 776 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 604 636 945 Size: 604 32 309 Fragments arranged by size: 604 309 32 MfeI C'AATT_G Cuts at: 0 737 945 Size: 737 208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 215 945 Size: 215 730 MslI CAynn'nnrTG Cuts at: 0 152 527 802 945 Size: 152 375 275 143 Fragments arranged by size: 375 275 152 143 MspA1I CmG'CkG Cuts at: 0 141 945 Size: 141 804 PfoI T'CCnGG_A Cuts at: 0 188 945 Size: 188 757 PpuMI rG'GwC_Cy Cuts at: 0 691 945 Size: 691 254 PsiI TTA'TAA Cuts at: 0 421 945 Size: 421 524 PvuII CAG'CTG Cuts at: 0 141 945 Size: 141 804 SacI G_AGCT'C Cuts at: 0 197 945 Size: 197 748 SmaI CCC'GGG Cuts at: 0 106 945 Size: 106 839 SmlI C'TyrA_G Cuts at: 0 760 945 Size: 760 185 SspI AAT'ATT Cuts at: 0 507 945 Size: 507 438 StyI C'CwwG_G Cuts at: 0 38 945 Size: 38 907 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 503 945 Size: 503 442 TatI w'GTAC_w Cuts at: 0 767 945 Size: 767 178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 165 945 Size: 165 780 XmaI C'CCGG_G Cuts at: 0 104 945 Size: 104 841 Enzymes that do cut: AcuI AleI AlfI ApoI AvaI BanII BbsI BbvCI BglII BplI BpmI Bpu10I BpuEI BsaI BsaHI BsiHKAI Bsp1286I BsrGI BstYI BtgI CspCI DraI DrdI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I MfeI MmeI MslI MspA1I PfoI PpuMI PsiI PvuII SacI SmaI SmlI SspI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraIII EaeI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI