Restriction map for
pF1KE0740
(Linear) (Six-base) MAPSORT of: KIEE0740.seq Check: 7886 from: 1 to: 1272
KIEE0740.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 477 897 1272
Size: 323 154 420 375
Fragments arranged by size:
420 375 323 154
AflIII A'CryG_T
Cuts at: 0 721 1272
Size: 721 551
ApoI r'AATT_y
Cuts at: 0 428 527 1009 1224 1272
Size: 428 99 482 215 48
Fragments arranged by size:
482 428 215 99 48
AseI AT'TA_AT
Cuts at: 0 182 1272
Size: 182 1090
AvaI C'yCGr_G
Cuts at: 0 22 1272
Size: 22 1250
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 854 887 1272
Size: 854 33 385
Fragments arranged by size:
854 385 33
BbvCI CC'TCA_GC
Cuts at: 0 112 1272
Size: 112 1160
BglII A'GATC_T
Cuts at: 0 572 1272
Size: 572 700
BmrI ACTGGGnnnn_n'
Cuts at: 0 876 1272
Size: 876 396
BmtI G_CTAG'C
Cuts at: 0 1180 1272
Size: 1180 92
Bpu10I CC'TnA_GC
Cuts at: 0 112 1272
Size: 112 1160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1272
Size: 56 1216
BsaAI yAC'GTr
Cuts at: 0 702 1272
Size: 702 570
BsaHI Gr'CG_yC
Cuts at: 0 4 19 1272
Size: 4 15 1253
Fragments arranged by size:
1253 15 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1272
Size: 91 1181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 233 669 1272
Size: 76 157 436 603
Fragments arranged by size:
603 436 157 76
BsrDI GCAATG_nn'
Cuts at: 0 242 1272
Size: 242 1030
BsrGI T'GTAC_A
Cuts at: 0 464 1272
Size: 464 808
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1118 1272
Size: 1118 154
BstYI r'GATC_y
Cuts at: 0 51 572 1272
Size: 51 521 700
Fragments arranged by size:
700 521 51
Bsu36I CC'TnA_GG
Cuts at: 0 595 857 1272
Size: 595 262 415
Fragments arranged by size:
595 415 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 992 1272
Size: 992 280
BtsI GCAGTG_nn'
Cuts at: 0 657 1272
Size: 657 615
DraI TTT'AAA
Cuts at: 0 1191 1272
Size: 1191 81
DraIII CAC_nnn'GTG
Cuts at: 0 899 1272
Size: 899 373
EarI CTCTTCn'nnn_
Cuts at: 0 901 940 1159 1272
Size: 901 39 219 113
Fragments arranged by size:
901 219 113 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 477 897 1272
Size: 323 154 420 375
Fragments arranged by size:
420 375 323 154
EcoO109I rG'GnC_Cy
Cuts at: 0 107 877 1100 1272
Size: 107 770 223 172
Fragments arranged by size:
770 223 172 107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1151 1272
Size: 1151 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 946 978 1272
Size: 564 32 350 32 294
Fragments arranged by size:
564 350 294 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1272
Size: 273 999
MslI CAynn'nnrTG
Cuts at: 0 554 620 830 1272
Size: 554 66 210 442
Fragments arranged by size:
554 442 210 66
NdeI CA'TA_TG
Cuts at: 0 730 1272
Size: 730 542
NheI G'CTAG_C
Cuts at: 0 1176 1272
Size: 1176 96
NspI r_CATG'y
Cuts at: 0 725 1121 1157 1176 1272
Size: 725 396 36 19 96
Fragments arranged by size:
725 396 96 36 19
PasI CC'CwG_GG
Cuts at: 0 30 443 1272
Size: 30 413 829
Fragments arranged by size:
829 413 30
PciI A'CATG_T
Cuts at: 0 721 1272
Size: 721 551
PpuMI rG'GwC_Cy
Cuts at: 0 107 1272
Size: 107 1165
PsiI TTA'TAA
Cuts at: 0 635 1272
Size: 635 637
PstI C_TGCA'G
Cuts at: 0 976 1272
Size: 976 296
SapI GCTCTTCn'nnn_
Cuts at: 0 901 940 1159 1272
Size: 901 39 219 113
Fragments arranged by size:
901 219 113 39
SfcI C'TryA_G
Cuts at: 0 972 1272
Size: 972 300
SmlI C'TyrA_G
Cuts at: 0 71 1272
Size: 71 1201
SphI G_CATG'C
Cuts at: 0 1157 1272
Size: 1157 115
SspI AAT'ATT
Cuts at: 0 314 403 1272
Size: 314 89 869
Fragments arranged by size:
869 314 89
TatI w'GTAC_w
Cuts at: 0 464 1272
Size: 464 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 1272
Size: 242 32 998
Fragments arranged by size:
998 242 32
XmnI GAAnn'nnTTC
Cuts at: 0 910 1213 1272
Size: 910 303 59
Fragments arranged by size:
910 303 59
Enzymes that do cut:
AcuI AflIII ApoI AseI AvaI BaeI BbvCI BglII
BmrI BmtI Bpu10I BpuEI BsaAI BsaHI BseRI BsgI
BsrDI BsrGI BstAPI BstYI Bsu36I BtgZI BtsI DraI
DraIII EarI Eco57MI EcoO109I FalI Hin4I MmeI MslI
NdeI NheI NspI PasI PciI PpuMI PsiI PstI
SapI SfcI SmlI SphI SspI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BplI
BpmI BsaI BsaBI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI
ClaI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI