Restriction map for
pF1KE0738
(Linear) (Six-base) MAPSORT of: KIEE0738.seq Check: 8900 from: 1 to: 1272
KIEE0738.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 1272
Size: 330 942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 876 1229 1272
Size: 474 402 353 43
Fragments arranged by size:
474 402 353 43
AfeI AGC'GCT
Cuts at: 0 196 1272
Size: 196 1076
AleI CACnn'nnGTG
Cuts at: 0 949 1272
Size: 949 323
AlwNI CAG_nnn'CTG
Cuts at: 0 264 276 995 1272
Size: 264 12 719 277
Fragments arranged by size:
719 277 264 12
ApaI G_GGCC'C
Cuts at: 0 307 1272
Size: 307 965
AvaI C'yCGr_G
Cuts at: 0 38 776 791 1116 1272
Size: 38 738 15 325 156
Fragments arranged by size:
738 325 156 38 15
BanI G'GyrC_C
Cuts at: 0 43 1249 1272
Size: 43 1206 23
Fragments arranged by size:
1206 43 23
BanII G_rGCy'C
Cuts at: 0 307 541 885 1272
Size: 307 234 344 387
Fragments arranged by size:
387 344 307 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 1272
Size: 498 774
BbvCI CC'TCA_GC
Cuts at: 0 1165 1171 1177 1272
Size: 1165 6 6 95
Fragments arranged by size:
1165 95 6 6
BglI GCCn_nnn'nGGC
Cuts at: 0 185 1272
Size: 185 1087
BglII A'GATC_T
Cuts at: 0 674 1272
Size: 674 598
BlpI GC'TnA_GC
Cuts at: 0 346 1272
Size: 346 926
Bme1580I G_kGCm'C
Cuts at: 0 46 307 1272
Size: 46 261 965
Fragments arranged by size:
965 261 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 750 1131 1272
Size: 412 338 381 141
Fragments arranged by size:
412 381 338 141
Bpu10I CC'TnA_GC
Cuts at: 0 540 762 1165 1171 1177 1272
Size: 540 222 403 6 6 95
Fragments arranged by size:
540 403 222 95 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1272
Size: 485 787
BsaI GGTCTCn'nnnn_
Cuts at: 0 1252 1272
Size: 1252 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 734 1272
Size: 106 628 538
Fragments arranged by size:
628 538 106
BseYI C'CCAG_C
Cuts at: 0 78 183 557 878 1076 1272
Size: 78 105 374 321 198 196
Fragments arranged by size:
374 321 198 196 105 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 339 1272
Size: 205 134 933
Fragments arranged by size:
933 205 134
BsiEI CG_ry'CG
Cuts at: 0 146 1272
Size: 146 1126
BsiHKAI G_wGCw'C
Cuts at: 0 347 1272
Size: 347 925
BsmI GAATG_Cn'
Cuts at: 0 930 1272
Size: 930 342
Bsp1286I G_dGCh'C
Cuts at: 0 46 307 347 541 885 1272
Size: 46 261 40 194 344 387
Fragments arranged by size:
387 344 261 194 46 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 344 461 1272
Size: 330 14 117 811
Fragments arranged by size:
811 330 117 14
BsrBI CCG'CTC
Cuts at: 0 1145 1272
Size: 1145 127
BsrFI r'CCGG_y
Cuts at: 0 630 1091 1272
Size: 630 461 181
Fragments arranged by size:
630 461 181
BstEII G'GTnAC_C
Cuts at: 0 633 1272
Size: 633 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 387 951 1272
Size: 132 255 564 321
Fragments arranged by size:
564 321 255 132
BstYI r'GATC_y
Cuts at: 0 674 827 1007 1272
Size: 674 153 180 265
Fragments arranged by size:
674 265 180 153
BtgI C'CryG_G
Cuts at: 0 245 586 1039 1272
Size: 245 341 453 233
Fragments arranged by size:
453 341 245 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 444 855 1272
Size: 444 411 417
Fragments arranged by size:
444 417 411
EaeI y'GGCC_r
Cuts at: 0 98 122 242 377 479 1157 1272
Size: 98 24 120 135 102 678 115
Fragments arranged by size:
678 135 120 115 102 98 24
EarI CTCTTCn'nnn_
Cuts at: 0 259 1205 1272
Size: 259 946 67
Fragments arranged by size:
946 259 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 474 750 876 1131 1229 1272
Size: 412 62 276 126 255 98 43
Fragments arranged by size:
412 276 255 126 98 62 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1272
Size: 523 749
EcoO109I rG'GnC_Cy
Cuts at: 0 303 304 746 1072 1272
Size: 303 1 442 326 200
Fragments arranged by size:
442 326 303 200 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 864 1272
Size: 864 408
FspI TGC'GCA
Cuts at: 0 421 1272
Size: 421 851
HaeII r_GCGC'y
Cuts at: 0 198 253 1272
Size: 198 55 1019
Fragments arranged by size:
1019 198 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 1272
Size: 733 32 507
Fragments arranged by size:
733 507 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1272
Size: 1127 145
MscI TGG'CCA
Cuts at: 0 100 124 379 1272
Size: 100 24 255 893
Fragments arranged by size:
893 255 100 24
MslI CAynn'nnrTG
Cuts at: 0 367 949 967 1272
Size: 367 582 18 305
Fragments arranged by size:
582 367 305 18
MspA1I CmG'CkG
Cuts at: 0 183 273 992 1041 1151 1255 1272
Size: 183 90 719 49 110 104 17
Fragments arranged by size:
719 183 110 104 90 49 17
NaeI GCC'GGC
Cuts at: 0 1093 1272
Size: 1093 179
NgoMIV G'CCGG_C
Cuts at: 0 1091 1272
Size: 1091 181
NspI r_CATG'y
Cuts at: 0 207 354 372 1272
Size: 207 147 18 900
Fragments arranged by size:
900 207 147 18
PasI CC'CwG_GG
Cuts at: 0 174 1272
Size: 174 1098
PpuMI rG'GwC_Cy
Cuts at: 0 1072 1272
Size: 1072 200
PshAI GACnn'nnGTC
Cuts at: 0 850 1272
Size: 850 422
PspOMI G'GGCC_C
Cuts at: 0 303 1272
Size: 303 969
PstI C_TGCA'G
Cuts at: 0 341 1272
Size: 341 931
PvuII CAG'CTG
Cuts at: 0 183 273 992 1151 1272
Size: 183 90 719 159 121
Fragments arranged by size:
719 183 159 121 90
SacII CC_GC'GG
Cuts at: 0 1042 1272
Size: 1042 230
SanDI GG'GwC_CC
Cuts at: 0 1072 1272
Size: 1072 200
SapI GCTCTTCn'nnn_
Cuts at: 0 259 1272
Size: 259 1013
SbfI CC_TGCA'GG
Cuts at: 0 341 1272
Size: 341 931
SfcI C'TryA_G
Cuts at: 0 337 654 1272
Size: 337 317 618
Fragments arranged by size:
618 337 317
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 185 1272
Size: 185 1087
SgrAI Cr'CCGG_yG
Cuts at: 0 1091 1272
Size: 1091 181
SmlI C'TyrA_G
Cuts at: 0 500 1116 1272
Size: 500 616 156
Fragments arranged by size:
616 500 156
SphI G_CATG'C
Cuts at: 0 207 1272
Size: 207 1065
StuI AGG'CCT
Cuts at: 0 706 1206 1272
Size: 706 500 66
Fragments arranged by size:
706 500 66
StyI C'CwwG_G
Cuts at: 0 101 577 1219 1272
Size: 101 476 642 53
Fragments arranged by size:
642 476 101 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 275 1272
Size: 275 997
XhoI C'TCGA_G
Cuts at: 0 1116 1272
Size: 1116 156
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BglI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BstEII BstXI BstYI
BtgI BtgZI EaeI EarI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PasI PpuMI PshAI PspOMI PstI PvuII
SacII SanDI SapI SbfI SfcI SfiI SgrAI SmlI
SphI StuI StyI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SalI ScaI SexAI SfoI
SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XmaI XmnI ZraI