Restriction map for
pF1KE0734
(Linear) (Six-base) MAPSORT of: KIEE0734.seq Check: 3158 from: 1 to: 1266
KIEE0734.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 280 1266
Size: 280 986
AccI GT'mk_AC
Cuts at: 0 519 1266
Size: 519 747
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1266
Size: 114 1152
ApaLI G'TGCA_C
Cuts at: 0 65 463 1266
Size: 65 398 803
Fragments arranged by size:
803 398 65
AvaI C'yCGr_G
Cuts at: 0 1049 1266
Size: 1049 217
AvrII C'CTAG_G
Cuts at: 0 708 1266
Size: 708 558
BanII G_rGCy'C
Cuts at: 0 173 359 1158 1266
Size: 173 186 799 108
Fragments arranged by size:
799 186 173 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 1252 1266
Size: 421 831 14
Fragments arranged by size:
831 421 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1078 1112 1266
Size: 1078 34 154
Fragments arranged by size:
1078 154 34
BlpI GC'TnA_GC
Cuts at: 0 1253 1266
Size: 1253 13
Bme1580I G_kGCm'C
Cuts at: 0 69 467 1266
Size: 69 398 799
Fragments arranged by size:
799 398 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 760 1266
Size: 760 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 989 1266
Size: 373 616 277
Fragments arranged by size:
616 373 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1266
Size: 745 521
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 984 1266
Size: 304 680 282
Fragments arranged by size:
680 304 282
BsaAI yAC'GTr
Cuts at: 0 238 462 1023 1266
Size: 238 224 561 243
Fragments arranged by size:
561 243 238 224
BsaBI GATnn'nnATC
Cuts at: 0 433 1266
Size: 433 833
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 1266
Size: 367 30 869
Fragments arranged by size:
869 367 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 865 1266
Size: 81 784 401
Fragments arranged by size:
784 401 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1266
Size: 308 958
BsiHKAI G_wGCw'C
Cuts at: 0 69 173 243 359 467 1266
Size: 69 104 70 116 108 799
Fragments arranged by size:
799 116 108 104 70 69
Bsp1286I G_dGCh'C
Cuts at: 0 69 173 243 359 467 1158 1266
Size: 69 104 70 116 108 691 108
Fragments arranged by size:
691 116 108 108 104 70 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 1266
Size: 280 986
BstBI TT'CG_AA
Cuts at: 0 1098 1266
Size: 1098 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 1266
Size: 351 915
BtgI C'CryG_G
Cuts at: 0 50 344 1148 1266
Size: 50 294 804 118
Fragments arranged by size:
804 294 118 50
BtsI GCAGTG_nn'
Cuts at: 0 69 291 1266
Size: 69 222 975
Fragments arranged by size:
975 222 69
EaeI y'GGCC_r
Cuts at: 0 569 1075 1266
Size: 569 506 191
Fragments arranged by size:
569 506 191
EarI CTCTTCn'nnn_
Cuts at: 0 934 1100 1266
Size: 934 166 166
Fragments arranged by size:
934 166 166
EcoICRI GAG'CTC
Cuts at: 0 171 357 1266
Size: 171 186 909
Fragments arranged by size:
909 186 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 373 989 1266
Size: 114 259 616 277
Fragments arranged by size:
616 277 259 114
EcoO109I rG'GnC_Cy
Cuts at: 0 121 1266
Size: 121 1145
EcoRV GAT'ATC
Cuts at: 0 787 1266
Size: 787 479
HaeII r_GCGC'y
Cuts at: 0 1260 1266
Size: 1260 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 1266
Size: 1063 32 171
Fragments arranged by size:
1063 171 32
HincII GTy'rAC
Cuts at: 0 520 1266
Size: 520 746
MscI TGG'CCA
Cuts at: 0 571 1266
Size: 571 695
MslI CAynn'nnrTG
Cuts at: 0 1163 1266
Size: 1163 103
MspA1I CmG'CkG
Cuts at: 0 441 1266
Size: 441 825
NcoI C'CATG_G
Cuts at: 0 50 344 1266
Size: 50 294 922
Fragments arranged by size:
922 294 50
NdeI CA'TA_TG
Cuts at: 0 716 1266
Size: 716 550
NspI r_CATG'y
Cuts at: 0 544 1266
Size: 544 722
PmlI CAC'GTG
Cuts at: 0 238 1266
Size: 238 1028
PpuMI rG'GwC_Cy
Cuts at: 0 121 1266
Size: 121 1145
PsiI TTA'TAA
Cuts at: 0 272 1266
Size: 272 994
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 466 498 1001 1033 1266
Size: 466 32 503 32 233
Fragments arranged by size:
503 466 233 32 32
PvuII CAG'CTG
Cuts at: 0 441 1266
Size: 441 825
SacI G_AGCT'C
Cuts at: 0 173 359 1266
Size: 173 186 907
Fragments arranged by size:
907 186 173
SalI G'TCGA_C
Cuts at: 0 518 1266
Size: 518 748
SanDI GG'GwC_CC
Cuts at: 0 121 1266
Size: 121 1145
SmlI C'TyrA_G
Cuts at: 0 760 1049 1266
Size: 760 289 217
Fragments arranged by size:
760 289 217
StyI C'CwwG_G
Cuts at: 0 50 344 360 572 708 1266
Size: 50 294 16 212 136 558
Fragments arranged by size:
558 294 212 136 50 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 413 1266
Size: 413 853
TatI w'GTAC_w
Cuts at: 0 260 1266
Size: 260 1006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 1266
Size: 1198 32 36
Fragments arranged by size:
1198 36 32
XhoI C'TCGA_G
Cuts at: 0 1049 1266
Size: 1049 217
Enzymes that do cut:
AarI AccI AcuI ApaLI AvaI AvrII BanII BbsI
BcgI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BstBI
BstXI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRV HaeII Hin4I HincII MscI MslI MspA1I NcoI
NdeI NspI PmlI PpuMI PsiI PsrI PvuII SacI
SalI SanDI SmlI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaHI
BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PspXI PstI PvuI RsrII SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XmaI XmnI ZraI