Restriction map for
pF1KE0729
(Linear) (Six-base) MAPSORT of: KIEE0729.seq Check: 2118 from: 1 to: 927
KIEE0729.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 883 927
Size: 232 651 44
Fragments arranged by size:
651 232 44
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 694 927
Size: 694 233
AlwNI CAG_nnn'CTG
Cuts at: 0 861 927
Size: 861 66
ApaI G_GGCC'C
Cuts at: 0 798 927
Size: 798 129
ApaLI G'TGCA_C
Cuts at: 0 565 927
Size: 565 362
ApoI r'AATT_y
Cuts at: 0 157 927
Size: 157 770
AvaI C'yCGr_G
Cuts at: 0 797 927
Size: 797 130
BanI G'GyrC_C
Cuts at: 0 107 927
Size: 107 820
BanII G_rGCy'C
Cuts at: 0 7 383 798 927
Size: 7 376 415 129
Fragments arranged by size:
415 376 129 7
BbeI G_GCGC'C
Cuts at: 0 111 927
Size: 111 816
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 927
Size: 595 332
Bme1580I G_kGCm'C
Cuts at: 0 569 798 927
Size: 569 229 129
Fragments arranged by size:
569 229 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 927
Size: 741 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 927
Size: 271 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 739 927
Size: 169 570 188
Fragments arranged by size:
570 188 169
BsaHI Gr'CG_yC
Cuts at: 0 108 138 927
Size: 108 30 789
Fragments arranged by size:
789 108 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 559 589 927
Size: 559 30 338
Fragments arranged by size:
559 338 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 597 927
Size: 457 140 330
Fragments arranged by size:
457 330 140
BseYI C'CCAG_C
Cuts at: 0 27 80 88 96 519 927
Size: 27 53 8 8 423 408
Fragments arranged by size:
423 408 53 27 8 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 927
Size: 43 884
BsiHKAI G_wGCw'C
Cuts at: 0 7 257 383 569 683 915 927
Size: 7 250 126 186 114 232 12
Fragments arranged by size:
250 232 186 126 114 12 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 257 383 569 683 798 915 927
Size: 7 250 126 186 114 115 117 12
Fragments arranged by size:
250 186 126 117 115 114 12 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 521 927
Size: 51 470 406
Fragments arranged by size:
470 406 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 927
Size: 261 666
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 927
Size: 18 909
BstYI r'GATC_y
Cuts at: 0 278 857 927
Size: 278 579 70
Fragments arranged by size:
579 278 70
Bsu36I CC'TnA_GG
Cuts at: 0 580 927
Size: 580 347
EarI CTCTTCn'nnn_
Cuts at: 0 47 226 927
Size: 47 179 701
Fragments arranged by size:
701 179 47
EcoICRI GAG'CTC
Cuts at: 0 5 381 927
Size: 5 376 546
Fragments arranged by size:
546 376 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 271 883 927
Size: 232 39 612 44
Fragments arranged by size:
612 232 44 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 579 927
Size: 148 431 348
Fragments arranged by size:
431 348 148
EcoO109I rG'GnC_Cy
Cuts at: 0 794 927
Size: 794 133
EcoRV GAT'ATC
Cuts at: 0 706 927
Size: 706 221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 927
Size: 237 690
HaeII r_GCGC'y
Cuts at: 0 111 725 927
Size: 111 614 202
Fragments arranged by size:
614 202 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 737 769 927
Size: 270 32 435 32 158
Fragments arranged by size:
435 270 158 32 32
KasI G'GCGC_C
Cuts at: 0 107 927
Size: 107 820
MslI CAynn'nnrTG
Cuts at: 0 676 927
Size: 676 251
MspA1I CmG'CkG
Cuts at: 0 27 261 927
Size: 27 234 666
Fragments arranged by size:
666 234 27
NarI GG'CG_CC
Cuts at: 0 108 927
Size: 108 819
NdeI CA'TA_TG
Cuts at: 0 645 927
Size: 645 282
PasI CC'CwG_GG
Cuts at: 0 144 872 927
Size: 144 728 55
Fragments arranged by size:
728 144 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 927
Size: 834 93
PspOMI G'GGCC_C
Cuts at: 0 794 927
Size: 794 133
PstI C_TGCA'G
Cuts at: 0 48 398 927
Size: 48 350 529
Fragments arranged by size:
529 350 48
PvuII CAG'CTG
Cuts at: 0 27 261 927
Size: 27 234 666
Fragments arranged by size:
666 234 27
SacI G_AGCT'C
Cuts at: 0 7 383 927
Size: 7 376 544
Fragments arranged by size:
544 376 7
SbfI CC_TGCA'GG
Cuts at: 0 48 927
Size: 48 879
SfcI C'TryA_G
Cuts at: 0 44 62 394 927
Size: 44 18 332 533
Fragments arranged by size:
533 332 44 18
SfoI GGC'GCC
Cuts at: 0 109 927
Size: 109 818
SmaI CCC'GGG
Cuts at: 0 799 927
Size: 799 128
SmlI C'TyrA_G
Cuts at: 0 184 754 927
Size: 184 570 173
Fragments arranged by size:
570 184 173
StyI C'CwwG_G
Cuts at: 0 370 833 927
Size: 370 463 94
Fragments arranged by size:
463 370 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 652 684 927
Size: 559 32 61 32 243
Fragments arranged by size:
559 243 61 32 32
XmaI C'CCGG_G
Cuts at: 0 797 927
Size: 797 130
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI Bme1580I BplI BpmI BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI
BstXI BstYI Bsu36I EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I KasI MslI MspA1I NarI
NdeI PasI PflMI PspOMI PstI PvuII SacI SbfI
SfcI SfoI SmaI SmlI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoRI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmnI ZraI