Restriction map for
pF1KE0726
(Linear) (Six-base) MAPSORT of: KIEE0726.seq Check: 2122 from: 1 to: 1251
KIEE0726.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 333 1251
Size: 333 918
AccI GT'mk_AC
Cuts at: 0 808 1251
Size: 808 443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1251
Size: 227 1024
AflII C'TTAA_G
Cuts at: 0 1219 1251
Size: 1219 32
AflIII A'CryG_T
Cuts at: 0 454 1174 1251
Size: 454 720 77
Fragments arranged by size:
720 454 77
AhdI GACnn_n'nnGTC
Cuts at: 0 605 1251
Size: 605 646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 1251
Size: 430 821
ApoI r'AATT_y
Cuts at: 0 111 867 971 1251
Size: 111 756 104 280
Fragments arranged by size:
756 280 111 104
BamHI G'GATC_C
Cuts at: 0 700 1251
Size: 700 551
BanI G'GyrC_C
Cuts at: 0 659 1251
Size: 659 592
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 530 701 1251
Size: 269 261 171 550
Fragments arranged by size:
550 269 261 171
BbvCI CC'TCA_GC
Cuts at: 0 173 1251
Size: 173 1078
BciVI GTATCCnnnnn_n'
Cuts at: 0 457 1251
Size: 457 794
Bme1580I G_kGCm'C
Cuts at: 0 714 1251
Size: 714 537
BmgBI CAC'GTC
Cuts at: 0 30 99 1251
Size: 30 69 1152
Fragments arranged by size:
1152 69 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 851 1251
Size: 851 400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 241 783 1251
Size: 241 542 468
Fragments arranged by size:
542 468 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 753 917 1001 1169 1251
Size: 288 465 164 84 168 82
Fragments arranged by size:
465 288 168 164 84 82
Bpu10I CC'TnA_GC
Cuts at: 0 173 1251
Size: 173 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1251
Size: 447 804
BsaAI yAC'GTr
Cuts at: 0 457 631 1251
Size: 457 174 620
Fragments arranged by size:
620 457 174
BsaWI w'CCGG_w
Cuts at: 0 871 1251
Size: 871 380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1251
Size: 67 1184
BseYI C'CCAG_C
Cuts at: 0 72 242 1251
Size: 72 170 1009
Fragments arranged by size:
1009 170 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1251
Size: 1087 164
BsmI GAATG_Cn'
Cuts at: 0 127 1251
Size: 127 1124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 89 1251
Size: 89 1162
Bsp1286I G_dGCh'C
Cuts at: 0 714 1251
Size: 714 537
BspHI T'CATG_A
Cuts at: 0 375 1251
Size: 375 876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 412 1251
Size: 333 79 839
Fragments arranged by size:
839 333 79
BsrDI GCAATG_nn'
Cuts at: 0 888 1251
Size: 888 363
BssSI C'ACGA_G
Cuts at: 0 228 1251
Size: 228 1023
BstAPI GCAn_nnn'nTGC
Cuts at: 0 853 1065 1251
Size: 853 212 186
Fragments arranged by size:
853 212 186
BstEII G'GTnAC_C
Cuts at: 0 15 1251
Size: 15 1236
BstYI r'GATC_y
Cuts at: 0 700 1251
Size: 700 551
BstZ17I GTA'TAC
Cuts at: 0 809 1251
Size: 809 442
Bsu36I CC'TnA_GG
Cuts at: 0 706 1251
Size: 706 545
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 1251
Size: 532 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 288 753 917 1001 1169 1251
Size: 227 61 465 164 84 168 82
Fragments arranged by size:
465 227 168 164 84 82 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 571 591 603 623 1251
Size: 431 32 108 20 12 20 628
Fragments arranged by size:
628 431 108 32 20 20 12
HindIII A'AGCT_T
Cuts at: 0 683 1251
Size: 683 568
MslI CAynn'nnrTG
Cuts at: 0 629 796 1232 1251
Size: 629 167 436 19
Fragments arranged by size:
629 436 167 19
MspA1I CmG'CkG
Cuts at: 0 666 1251
Size: 666 585
NdeI CA'TA_TG
Cuts at: 0 965 1251
Size: 965 286
NspI r_CATG'y
Cuts at: 0 1063 1178 1251
Size: 1063 115 73
Fragments arranged by size:
1063 115 73
PciI A'CATG_T
Cuts at: 0 1174 1251
Size: 1174 77
PmlI CAC'GTG
Cuts at: 0 457 1251
Size: 457 794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 1251
Size: 222 32 997
Fragments arranged by size:
997 222 32
PstI C_TGCA'G
Cuts at: 0 330 409 1251
Size: 330 79 842
Fragments arranged by size:
842 330 79
SbfI CC_TGCA'GG
Cuts at: 0 409 1251
Size: 409 842
SfcI C'TryA_G
Cuts at: 0 90 326 405 1251
Size: 90 236 79 846
Fragments arranged by size:
846 236 90 79
SmlI C'TyrA_G
Cuts at: 0 462 1219 1251
Size: 462 757 32
Fragments arranged by size:
757 462 32
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AlfI ApoI
BamHI BanI BbsI BbvCI BciVI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI
BstAPI BstEII BstYI BstZ17I Bsu36I DrdI Eco57MI Hin4I
HindIII MslI MspA1I NdeI NspI PciI PmlI PpiI
PstI SbfI SfcI SmlI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BcgI BclI BfrBI BglI BglII BlpI
BmtI BsaI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BspEI BsrBI BsrFI BsrGI BssHII BstBI BstXI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI