Restriction map for
pF1KE0715
(Linear) (Six-base) MAPSORT of: KIEE0715.seq Check: 1883 from: 1 to: 912
KIEE0715.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 0 578 912
Size: 578 334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 745 912
Size: 515 230 167
Fragments arranged by size:
515 230 167
AleI CACnn'nnGTG
Cuts at: 0 649 912
Size: 649 263
AlwNI CAG_nnn'CTG
Cuts at: 0 646 912
Size: 646 266
AvaI C'yCGr_G
Cuts at: 0 305 912
Size: 305 607
BanII G_rGCy'C
Cuts at: 0 20 796 912
Size: 20 776 116
Fragments arranged by size:
776 116 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 912
Size: 896 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 436 912
Size: 319 117 476
Fragments arranged by size:
476 319 117
BsaHI Gr'CG_yC
Cuts at: 0 575 912
Size: 575 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 912
Size: 535 30 347
Fragments arranged by size:
535 347 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 352 361 912
Size: 70 282 9 551
Fragments arranged by size:
551 282 70 9
BsiEI CG_ry'CG
Cuts at: 0 854 912
Size: 854 58
BsiHKAI G_wGCw'C
Cuts at: 0 20 361 912
Size: 20 341 551
Fragments arranged by size:
551 341 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 361 796 912
Size: 20 341 435 116
Fragments arranged by size:
435 341 116 20
BspHI T'CATG_A
Cuts at: 0 830 912
Size: 830 82
BsrBI CCG'CTC
Cuts at: 0 312 858 912
Size: 312 546 54
Fragments arranged by size:
546 312 54
BtgI C'CryG_G
Cuts at: 0 761 912
Size: 761 151
BtsI GCAGTG_nn'
Cuts at: 0 11 644 912
Size: 11 633 268
Fragments arranged by size:
633 268 11
EaeI y'GGCC_r
Cuts at: 0 851 912
Size: 851 61
EagI C'GGCC_G
Cuts at: 0 851 912
Size: 851 61
EarI CTCTTCn'nnn_
Cuts at: 0 160 338 392 912
Size: 160 178 54 520
Fragments arranged by size:
520 178 160 54
EcoICRI GAG'CTC
Cuts at: 0 18 912
Size: 18 894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 436 515 745 912
Size: 319 117 79 230 167
Fragments arranged by size:
319 230 167 117 79
EcoO109I rG'GnC_Cy
Cuts at: 0 102 254 912
Size: 102 152 658
Fragments arranged by size:
658 152 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 307 912
Size: 36 271 605
Fragments arranged by size:
605 271 36
FspI TGC'GCA
Cuts at: 0 838 912
Size: 838 74
FspAI rTGC'GCAy
Cuts at: 0 838 912
Size: 838 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 912
Size: 535 32 345
Fragments arranged by size:
535 345 32
HindIII A'AGCT_T
Cuts at: 0 289 912
Size: 289 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 912
Size: 719 193
MslI CAynn'nnrTG
Cuts at: 0 649 912
Size: 649 263
MspA1I CmG'CkG
Cuts at: 0 67 148 414 679 867 912
Size: 67 81 266 265 188 45
Fragments arranged by size:
266 265 188 81 67 45
NdeI CA'TA_TG
Cuts at: 0 213 912
Size: 213 699
NspI r_CATG'y
Cuts at: 0 843 912
Size: 843 69
PfoI T'CCnGG_A
Cuts at: 0 437 912
Size: 437 475
PpuMI rG'GwC_Cy
Cuts at: 0 254 912
Size: 254 658
PstI C_TGCA'G
Cuts at: 0 201 912
Size: 201 711
PvuII CAG'CTG
Cuts at: 0 148 414 867 912
Size: 148 266 453 45
Fragments arranged by size:
453 266 148 45
SacI G_AGCT'C
Cuts at: 0 20 912
Size: 20 892
SexAI A'CCwGG_T
Cuts at: 0 617 912
Size: 617 295
SfcI C'TryA_G
Cuts at: 0 197 912
Size: 197 715
SmaI CCC'GGG
Cuts at: 0 307 912
Size: 307 605
SphI G_CATG'C
Cuts at: 0 843 912
Size: 843 69
StuI AGG'CCT
Cuts at: 0 175 912
Size: 175 737
StyI C'CwwG_G
Cuts at: 0 11 176 501 912
Size: 11 165 325 411
Fragments arranged by size:
411 325 165 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 205 912
Size: 131 74 707
Fragments arranged by size:
707 131 74
Tth111I GACn'n_nGTC
Cuts at: 0 220 912
Size: 220 692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 912
Size: 700 212
XmaI C'CCGG_G
Cuts at: 0 305 912
Size: 305 607
ZraI GAC'GTC
Cuts at: 0 576 912
Size: 576 336
Enzymes that do cut:
AatII AcuI AleI AlwNI AvaI BanII BbsI BpmI
BsaHI BsaXI BseRI BsiEI BsiHKAI Bsp1286I BspHI BsrBI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
FalI FspI FspAI Hin4I HindIII MmeI MslI MspA1I
NdeI NspI PfoI PpuMI PstI PvuII SacI SexAI
SfcI SmaI SphI StuI StyI TaqII Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BseYI BsgI BsiWI BsmI BsmBI BspEI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoRI EcoRV FseI HaeII
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TatI TstI XbaI
XhoI XmnI