Restriction map for
pF1KE0711
(Linear) (Six-base) MAPSORT of: KIEE0711.seq Check: 5228 from: 1 to: 1269
KIEE0711.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 686 1269
Size: 686 583
AccI GT'mk_AC
Cuts at: 0 908 1269
Size: 908 361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1269
Size: 595 674
AleI CACnn'nnGTG
Cuts at: 0 14 1269
Size: 14 1255
AlwNI CAG_nnn'CTG
Cuts at: 0 765 1269
Size: 765 504
ApaI G_GGCC'C
Cuts at: 0 245 1135 1269
Size: 245 890 134
Fragments arranged by size:
890 245 134
AvaI C'yCGr_G
Cuts at: 0 629 1269
Size: 629 640
BanI G'GyrC_C
Cuts at: 0 285 674 755 1269
Size: 285 389 81 514
Fragments arranged by size:
514 389 285 81
BanII G_rGCy'C
Cuts at: 0 139 245 830 1135 1153 1269
Size: 139 106 585 305 18 116
Fragments arranged by size:
585 305 139 116 106 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 534 1269
Size: 81 453 735
Fragments arranged by size:
735 453 81
BbvCI CC'TCA_GC
Cuts at: 0 712 1269
Size: 712 557
BclI T'GATC_A
Cuts at: 0 422 1269
Size: 422 847
BglI GCCn_nnn'nGGC
Cuts at: 0 283 634 1130 1269
Size: 283 351 496 139
Fragments arranged by size:
496 351 283 139
BlpI GC'TnA_GC
Cuts at: 0 601 1269
Size: 601 668
Bme1580I G_kGCm'C
Cuts at: 0 245 677 730 1135 1269
Size: 245 432 53 405 134
Fragments arranged by size:
432 405 245 134 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 1129 1269
Size: 1129 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 257 322 796 1149 1269
Size: 64 193 65 474 353 120
Fragments arranged by size:
474 353 193 120 65 64
Bpu10I CC'TnA_GC
Cuts at: 0 53 133 685 712 1269
Size: 53 80 552 27 557
Fragments arranged by size:
557 552 80 53 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 1242 1269
Size: 460 782 27
Fragments arranged by size:
782 460 27
BsaAI yAC'GTr
Cuts at: 0 597 1269
Size: 597 672
BsaWI w'CCGG_w
Cuts at: 0 836 1269
Size: 836 433
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1135 1147 1165 1177 1269
Size: 1135 12 18 12 92
Fragments arranged by size:
1135 92 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 147 150 186 448 655 1248 1269
Size: 147 3 36 262 207 593 21
Fragments arranged by size:
593 262 207 147 36 21 3
BseYI C'CCAG_C
Cuts at: 0 928 1107 1269
Size: 928 179 162
Fragments arranged by size:
928 179 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 981 1246 1269
Size: 604 377 265 23
Fragments arranged by size:
604 377 265 23
BsiHKAI G_wGCw'C
Cuts at: 0 59 139 691 1061 1153 1269
Size: 59 80 552 370 92 116
Fragments arranged by size:
552 370 116 92 80 59
Bsp1286I G_dGCh'C
Cuts at: 0 59 139 245 677 691 730 830 1061
Size: 59 80 106 432 14 39 100 231
Cuts at: 1061 1135 1153 1269
Size: 74 18 116
Fragments arranged by size:
432 231 116 106 100 80 74 59
39 18 14
BspEI T'CCGG_A
Cuts at: 0 836 1269
Size: 836 433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 686 779 1269
Size: 440 246 93 490
Fragments arranged by size:
490 440 246 93
BsrBI CCG'CTC
Cuts at: 0 983 1269
Size: 983 286
BsrFI r'CCGG_y
Cuts at: 0 1124 1269
Size: 1124 145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1269
Size: 900 369
BtgI C'CryG_G
Cuts at: 0 12 100 116 614 843 893 953 1011
Size: 12 88 16 498 229 50 60 58
Cuts at: 1011 1269
Size: 258
Fragments arranged by size:
498 258 229 88 60 58 50 16
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 1269
Size: 540 729
BtsI GCAGTG_nn'
Cuts at: 0 1177 1269
Size: 1177 92
DraIII CAC_nnn'GTG
Cuts at: 0 597 1269
Size: 597 672
EaeI y'GGCC_r
Cuts at: 0 18 404 530 635 890 902 1269
Size: 18 386 126 105 255 12 367
Fragments arranged by size:
386 367 255 126 105 18 12
EarI CTCTTCn'nnn_
Cuts at: 0 264 346 511 1012 1269
Size: 264 82 165 501 257
Fragments arranged by size:
501 264 257 165 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 963 1216 1269
Size: 963 253 53
Fragments arranged by size:
963 253 53
EcoICRI GAG'CTC
Cuts at: 0 137 1151 1269
Size: 137 1014 118
Fragments arranged by size:
1014 137 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 257 322 595 796 1149 1269
Size: 64 193 65 273 201 353 120
Fragments arranged by size:
353 273 201 193 120 65 64
EcoO109I rG'GnC_Cy
Cuts at: 0 230 241 242 1131 1269
Size: 230 11 1 889 138
Fragments arranged by size:
889 230 138 11 1
HaeII r_GCGC'y
Cuts at: 0 477 799 1179 1269
Size: 477 322 380 90
Fragments arranged by size:
477 380 322 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1269
Size: 1135 32 102
Fragments arranged by size:
1135 102 32
HincII GTy'rAC
Cuts at: 0 501 1269
Size: 501 768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1269
Size: 401 868
MscI TGG'CCA
Cuts at: 0 20 892 1269
Size: 20 872 377
Fragments arranged by size:
872 377 20
MslI CAynn'nnrTG
Cuts at: 0 14 883 898 1269
Size: 14 869 15 371
Fragments arranged by size:
869 371 15 14
MspA1I CmG'CkG
Cuts at: 0 24 409 625 752 1269
Size: 24 385 216 127 517
Fragments arranged by size:
517 385 216 127 24
NaeI GCC'GGC
Cuts at: 0 1126 1269
Size: 1126 143
NcoI C'CATG_G
Cuts at: 0 100 116 893 953 1011 1269
Size: 100 16 777 60 58 258
Fragments arranged by size:
777 258 100 60 58 16
NgoMIV G'CCGG_C
Cuts at: 0 1124 1269
Size: 1124 145
PasI CC'CwG_GG
Cuts at: 0 1263 1269
Size: 1263 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 1172 1269
Size: 1172 97
PfoI T'CCnGG_A
Cuts at: 0 1046 1269
Size: 1046 223
PmlI CAC'GTG
Cuts at: 0 597 1269
Size: 597 672
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1269
Size: 76 32 1161
Fragments arranged by size:
1161 76 32
PpuMI rG'GwC_Cy
Cuts at: 0 230 1269
Size: 230 1039
PspOMI G'GGCC_C
Cuts at: 0 241 1131 1269
Size: 241 890 138
Fragments arranged by size:
890 241 138
PstI C_TGCA'G
Cuts at: 0 67 1269
Size: 67 1202
PvuII CAG'CTG
Cuts at: 0 24 625 1269
Size: 24 601 644
Fragments arranged by size:
644 601 24
SacI G_AGCT'C
Cuts at: 0 139 1153 1269
Size: 139 1014 116
Fragments arranged by size:
1014 139 116
SapI GCTCTTCn'nnn_
Cuts at: 0 1012 1269
Size: 1012 257
SbfI CC_TGCA'GG
Cuts at: 0 67 1269
Size: 67 1202
SfcI C'TryA_G
Cuts at: 0 63 717 1269
Size: 63 654 552
Fragments arranged by size:
654 552 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1130 1269
Size: 1130 139
SmaI CCC'GGG
Cuts at: 0 631 1269
Size: 631 638
StuI AGG'CCT
Cuts at: 0 1189 1269
Size: 1189 80
StyI C'CwwG_G
Cuts at: 0 100 116 893 953 1011 1269
Size: 100 16 777 60 58 258
Fragments arranged by size:
777 258 100 60 58 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 1269
Size: 639 630
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 635 667 992 1024 1269
Size: 635 32 325 32 245
Fragments arranged by size:
635 325 245 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 885 1269
Size: 114 771 384
Fragments arranged by size:
771 384 114
XmaI C'CCGG_G
Cuts at: 0 629 1269
Size: 629 640
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstXI
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SapI SbfI
SfcI SfiI SmaI StuI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglII BmgBI
BmtI BplI BpuEI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI