Restriction map for
pF1KE0708
(Linear) (Six-base) MAPSORT of: KIEE0708.seq Check: 2167 from: 1 to: 1221
KIEE0708.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 1221
Size: 238 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 747 1221
Size: 495 252 474
Fragments arranged by size:
495 474 252
AflIII A'CryG_T
Cuts at: 0 805 1221
Size: 805 416
AleI CACnn'nnGTG
Cuts at: 0 275 359 1221
Size: 275 84 862
Fragments arranged by size:
862 275 84
AlwNI CAG_nnn'CTG
Cuts at: 0 351 1221
Size: 351 870
ApoI r'AATT_y
Cuts at: 0 64 863 907 1162 1221
Size: 64 799 44 255 59
Fragments arranged by size:
799 255 64 59 44
BanI G'GyrC_C
Cuts at: 0 189 1221
Size: 189 1032
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 501 1221
Size: 56 445 720
Fragments arranged by size:
720 445 56
BfrBI ATG'CAT
Cuts at: 0 650 1221
Size: 650 571
Bme1580I G_kGCm'C
Cuts at: 0 37 1221
Size: 37 1184
BmgBI CAC'GTC
Cuts at: 0 54 1221
Size: 54 1167
BmrI ACTGGGnnnn_n'
Cuts at: 0 584 1221
Size: 584 637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 538 893 977 1077 1145 1221
Size: 151 387 355 84 100 68 76
Fragments arranged by size:
387 355 151 100 84 76 68
Bpu10I CC'TnA_GC
Cuts at: 0 16 538 1221
Size: 16 522 683
Fragments arranged by size:
683 522 16
BsaAI yAC'GTr
Cuts at: 0 806 1221
Size: 806 415
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 1221
Size: 448 30 743
Fragments arranged by size:
743 448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 1221
Size: 469 752
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1080 1164 1221
Size: 337 743 84 57
Fragments arranged by size:
743 337 84 57
BsiEI CG_ry'CG
Cuts at: 0 11 1221
Size: 11 1210
BsiHKAI G_wGCw'C
Cuts at: 0 422 1221
Size: 422 799
BsmI GAATG_Cn'
Cuts at: 0 979 1221
Size: 979 242
Bsp1286I G_dGCh'C
Cuts at: 0 37 422 1221
Size: 37 385 799
Fragments arranged by size:
799 385 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 1221
Size: 238 983
BsrDI GCAATG_nn'
Cuts at: 0 932 1221
Size: 932 289
BsrGI T'GTAC_A
Cuts at: 0 852 1221
Size: 852 369
BssSI C'ACGA_G
Cuts at: 0 688 1221
Size: 688 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 1221
Size: 351 870
BtsI GCAGTG_nn'
Cuts at: 0 984 1068 1152 1221
Size: 984 84 84 69
Fragments arranged by size:
984 84 84 69
EaeI y'GGCC_r
Cuts at: 0 8 1221
Size: 8 1213
EagI C'GGCC_G
Cuts at: 0 8 1221
Size: 8 1213
EarI CTCTTCn'nnn_
Cuts at: 0 466 1182 1221
Size: 466 716 39
Fragments arranged by size:
716 466 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 495 538 747 893 977 1077 1145
Size: 151 344 43 209 146 84 100 68
Cuts at: 1145 1221
Size: 76
Fragments arranged by size:
344 209 151 146 100 84 76 68
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 245 1221
Size: 94 151 976
Fragments arranged by size:
976 151 94
EcoRI G'AATT_C
Cuts at: 0 863 1221
Size: 863 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 1221
Size: 589 32 600
Fragments arranged by size:
600 589 32
MslI CAynn'nnrTG
Cuts at: 0 275 359 413 888 972 1221
Size: 275 84 54 475 84 249
Fragments arranged by size:
475 275 249 84 84 54
MspA1I CmG'CkG
Cuts at: 0 638 1221
Size: 638 583
NdeI CA'TA_TG
Cuts at: 0 885 969 1221
Size: 885 84 252
Fragments arranged by size:
885 252 84
NotI GC'GGCC_GC
Cuts at: 0 8 1221
Size: 8 1213
NsiI A_TGCA'T
Cuts at: 0 652 1221
Size: 652 569
NspI r_CATG'y
Cuts at: 0 316 490 1221
Size: 316 174 731
Fragments arranged by size:
731 316 174
PasI CC'CwG_GG
Cuts at: 0 156 1221
Size: 156 1065
PshAI GACnn'nnGTC
Cuts at: 0 229 1221
Size: 229 992
PvuII CAG'CTG
Cuts at: 0 638 1221
Size: 638 583
SfcI C'TryA_G
Cuts at: 0 438 704 1221
Size: 438 266 517
Fragments arranged by size:
517 438 266
StuI AGG'CCT
Cuts at: 0 367 1221
Size: 367 854
TatI w'GTAC_w
Cuts at: 0 852 1221
Size: 852 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 637 1221
Size: 637 584
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApoI BanI BbsI
BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrGI BssSI BstAPI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoRI Hin4I MslI MspA1I NdeI NotI NsiI
NspI PasI PshAI PvuII SfcI StuI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsaWI BseYI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NruI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI