Restriction map for
pF1KE0707
(Linear) (Six-base) MAPSORT of: KIEE0707.seq Check: 23 from: 1 to: 1278
KIEE0707.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 853 1278
Size: 853 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1278
Size: 282 996
ApaI G_GGCC'C
Cuts at: 0 161 528 634 678 933 1278
Size: 161 367 106 44 255 345
Fragments arranged by size:
367 345 255 161 106 44
ApaLI G'TGCA_C
Cuts at: 0 490 1009 1278
Size: 490 519 269
Fragments arranged by size:
519 490 269
AvaI C'yCGr_G
Cuts at: 0 1117 1278
Size: 1117 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 133 166 1278
Size: 133 33 1112
Fragments arranged by size:
1112 133 33
BanII G_rGCy'C
Cuts at: 0 161 353 528 634 678 820 933 1045
Size: 161 192 175 106 44 142 113 112
Cuts at: 1045 1207 1278
Size: 162 71
Fragments arranged by size:
192 175 162 161 142 113 112 106
71 44
BbvCI CC'TCA_GC
Cuts at: 0 1165 1278
Size: 1165 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 881 1278
Size: 847 34 397
Fragments arranged by size:
847 397 34
BglI GCCn_nnn'nGGC
Cuts at: 0 599 1278
Size: 599 679
Bme1580I G_kGCm'C
Cuts at: 0 101 161 494 528 623 634 678 933
Size: 101 60 333 34 95 11 44 255
Cuts at: 933 1013 1278
Size: 80 265
Fragments arranged by size:
333 265 255 101 95 80 60 44
34 11
BmgBI CAC'GTC
Cuts at: 0 1014 1278
Size: 1014 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 628 1278
Size: 628 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 367 484 838 1278
Size: 56 311 117 354 440
Fragments arranged by size:
440 354 311 117 56
Bpu10I CC'TnA_GC
Cuts at: 0 463 1165 1278
Size: 463 702 113
Fragments arranged by size:
702 463 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1278
Size: 130 1148
BsaI GGTCTCn'nnnn_
Cuts at: 0 32 1087 1258 1278
Size: 32 1055 171 20
Fragments arranged by size:
1055 171 32 20
BsaAI yAC'GTr
Cuts at: 0 618 1008 1278
Size: 618 390 270
Fragments arranged by size:
618 390 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1003 1033 1096 1126 1278
Size: 1003 30 63 30 152
Fragments arranged by size:
1003 152 63 30 30
BseYI C'CCAG_C
Cuts at: 0 161 384 926 1217 1234 1278
Size: 161 223 542 291 17 44
Fragments arranged by size:
542 291 223 161 44 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 598 715 973 1278
Size: 580 18 117 258 305
Fragments arranged by size:
580 305 258 117 18
BsiEI CG_ry'CG
Cuts at: 0 867 948 1278
Size: 867 81 330
Fragments arranged by size:
867 330 81
BsiHKAI G_wGCw'C
Cuts at: 0 353 449 494 749 1013 1064 1278
Size: 353 96 45 255 264 51 214
Fragments arranged by size:
353 264 255 214 96 51 45
Bsp1286I G_dGCh'C
Cuts at: 0 101 161 353 449 494 528 623 634
Size: 101 60 192 96 45 34 95 11
Cuts at: 634 678 749 820 933 1013 1045 1064 1207
Size: 44 71 71 113 80 32 19 143
Cuts at: 1207 1278
Size: 71
Fragments arranged by size:
192 143 113 101 96 95 80 71
71 71 60 45 44 34 32 19
11
BspHI T'CATG_A
Cuts at: 0 737 1278
Size: 737 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 326 761 853 1278
Size: 209 117 435 92 425
Fragments arranged by size:
435 425 209 117 92
BsrDI GCAATG_nn'
Cuts at: 0 84 237 1278
Size: 84 153 1041
Fragments arranged by size:
1041 153 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 1278
Size: 513 765
Bsu36I CC'TnA_GG
Cuts at: 0 110 1278
Size: 110 1168
BtgI C'CryG_G
Cuts at: 0 68 485 512 670 728 1278
Size: 68 417 27 158 58 550
Fragments arranged by size:
550 417 158 68 58 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1117 1278
Size: 1117 161
BtsI GCAGTG_nn'
Cuts at: 0 90 455 1278
Size: 90 365 823
Fragments arranged by size:
823 365 90
DraIII CAC_nnn'GTG
Cuts at: 0 1193 1278
Size: 1193 85
EaeI y'GGCC_r
Cuts at: 0 89 305 482 731 1049 1278
Size: 89 216 177 249 318 229
Fragments arranged by size:
318 249 229 216 177 89
EarI CTCTTCn'nnn_
Cuts at: 0 301 718 1278
Size: 301 417 560
Fragments arranged by size:
560 417 301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 655 1278
Size: 655 623
EcoICRI GAG'CTC
Cuts at: 0 351 1278
Size: 351 927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 282 367 484 838 1278
Size: 56 226 85 117 354 440
Fragments arranged by size:
440 354 226 117 85 56
EcoO109I rG'GnC_Cy
Cuts at: 0 157 158 630 675 930 1107 1121 1278
Size: 157 1 472 45 255 177 14 157
Fragments arranged by size:
472 255 177 157 157 45 14 1
FspI TGC'GCA
Cuts at: 0 274 1278
Size: 274 1004
FspAI rTGC'GCAy
Cuts at: 0 274 1278
Size: 274 1004
HaeII r_GCGC'y
Cuts at: 0 224 1278
Size: 224 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1278
Size: 1189 32 57
Fragments arranged by size:
1189 57 32
MfeI C'AATT_G
Cuts at: 0 18 1278
Size: 18 1260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1278
Size: 293 985
MscI TGG'CCA
Cuts at: 0 733 1278
Size: 733 545
MslI CAynn'nnrTG
Cuts at: 0 691 742 1278
Size: 691 51 536
Fragments arranged by size:
691 536 51
MspA1I CmG'CkG
Cuts at: 0 612 649 1217 1278
Size: 612 37 568 61
Fragments arranged by size:
612 568 61 37
NcoI C'CATG_G
Cuts at: 0 68 512 728 1278
Size: 68 444 216 550
Fragments arranged by size:
550 444 216 68
NspI r_CATG'y
Cuts at: 0 660 1278
Size: 660 618
PasI CC'CwG_GG
Cuts at: 0 49 1209 1278
Size: 49 1160 69
Fragments arranged by size:
1160 69 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 1230 1278
Size: 1230 48
PfoI T'CCnGG_A
Cuts at: 0 1100 1278
Size: 1100 178
PmlI CAC'GTG
Cuts at: 0 618 1008 1278
Size: 618 390 270
Fragments arranged by size:
618 390 270
PspOMI G'GGCC_C
Cuts at: 0 157 524 630 674 929 1278
Size: 157 367 106 44 255 349
Fragments arranged by size:
367 349 255 157 106 44
PstI C_TGCA'G
Cuts at: 0 825 850 1278
Size: 825 25 428
Fragments arranged by size:
825 428 25
PvuI CG_AT'CG
Cuts at: 0 867 1278
Size: 867 411
PvuII CAG'CTG
Cuts at: 0 612 649 1217 1278
Size: 612 37 568 61
Fragments arranged by size:
612 568 61 37
SacI G_AGCT'C
Cuts at: 0 353 1278
Size: 353 925
SapI GCTCTTCn'nnn_
Cuts at: 0 718 1278
Size: 718 560
SbfI CC_TGCA'GG
Cuts at: 0 850 1278
Size: 850 428
SfcI C'TryA_G
Cuts at: 0 821 846 1065 1278
Size: 821 25 219 213
Fragments arranged by size:
821 219 213 25
SmlI C'TyrA_G
Cuts at: 0 145 1278
Size: 145 1133
SphI G_CATG'C
Cuts at: 0 660 1278
Size: 660 618
StyI C'CwwG_G
Cuts at: 0 68 512 728 806 1278
Size: 68 444 216 78 472
Fragments arranged by size:
472 444 216 78 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 1278
Size: 509 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1003 1035 1096 1128 1278
Size: 1003 32 61 32 150
Fragments arranged by size:
1003 150 61 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 949 1278
Size: 949 329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1231 1278
Size: 1231 47
Enzymes that do cut:
AarI AcuI ApaI ApaLI AvaI BaeI BanII BbvCI
BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I MfeI MmeI MscI MslI MspA1I
NcoI NspI PasI PflMI PfoI PmlI PspOMI PstI
PvuI PvuII SacI SapI SbfI SfcI SmlI SphI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BamHI BanI BbeI BbsI BciVI BclI
BfrBI BglII BlpI BmtI BplI BsaBI BsaHI BsaWI
BseRI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI XbaI XhoI XmaI XmnI ZraI