(Linear) (Six-base) MAPSORT of: KIEE0703.seq  Check: 337  from: 1  to: 879



KIEE0703.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AatII G_ACGT'C

Cuts at:      0     610     879 
   Size:        610     269
 
AccI GT'mk_AC

Cuts at:      0     610     879 
   Size:        610     269
 
ApaI G_GGCC'C

Cuts at:      0     697     879 
   Size:        697     182
 
BanII G_rGCy'C

Cuts at:      0     697     879 
   Size:        697     182
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     682     879 
   Size:        682     197
 
BfrBI ATG'CAT

Cuts at:      0     265     879 
   Size:        265     614
 
BglII A'GATC_T

Cuts at:      0     179     879 
   Size:        179     700
 
Bme1580I G_kGCm'C

Cuts at:      0     697     879 
   Size:        697     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     192     879 
   Size:        192     687
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     879 
   Size:        152     727
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725     879 
   Size:        725     154
 
BsaAI yAC'GTr

Cuts at:      0     631     879 
   Size:        631     248
 
BsaHI Gr'CG_yC

Cuts at:      0     607     879 
   Size:        607     272
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     295     879 
   Size:        265      30     584

  Fragments arranged by size: 

                 584     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     499     879 
   Size:        499     380
 
BseYI C'CCAG_C

Cuts at:      0      22     101     879 
   Size:         22      79     778

  Fragments arranged by size: 

                 778      79      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     697     879 
   Size:        697     182
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      72     879 
   Size:         72     807
 
BsrGI T'GTAC_A

Cuts at:      0     709     879 
   Size:        709     170
 
BstYI r'GATC_y

Cuts at:      0     179     879 
   Size:        179     700
 
DraI TTT'AAA

Cuts at:      0     393     495     879 
   Size:        393     102     384

  Fragments arranged by size: 

                 393     384     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     879 
   Size:        152     727
 
EcoO109I rG'GnC_Cy

Cuts at:      0     693     694     879 
   Size:        693       1     185

  Fragments arranged by size: 

                 693     185       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     139     879 
   Size:        139     740
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     879 
   Size:        265      32     582

  Fragments arranged by size: 

                 582     265      32
 
NsiI A_TGCA'T

Cuts at:      0     267     879 
   Size:        267     612
 
PfoI T'CCnGG_A

Cuts at:      0     129     879 
   Size:        129     750
 
PspOMI G'GGCC_C

Cuts at:      0     693     879 
   Size:        693     186
 
SmlI C'TyrA_G

Cuts at:      0     740     879 
   Size:        740     139
 
SnaBI TAC'GTA

Cuts at:      0     631     879 
   Size:        631     248
 
SspI AAT'ATT

Cuts at:      0     405     879 
   Size:        405     474
 
TatI w'GTAC_w

Cuts at:      0     375     709     879 
   Size:        375     334     170

  Fragments arranged by size: 

                 375     334     170
 
ZraI GAC'GTC

Cuts at:      0     608     879 
   Size:        608     271
 

 Enzymes that do cut:

    AatII     AccI     ApaI    BanII     BbsI    BfrBI    BglII Bme1580I
     BmrI     BpmI    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
 Bsp1286I    BspMI    BsrGI    BstYI     DraI  Eco57MI EcoO109I     FalI
    Hin4I     NsiI     PfoI   PspOMI     SmlI    SnaBI     SspI     TatI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI