Restriction map for
pF1KE0702
(Linear) (Six-base) MAPSORT of: KIEE0702.seq Check: 7132 from: 1 to: 1218
KIEE0702.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 807 1218
Size: 807 411
AatII G_ACGT'C
Cuts at: 0 86 1218
Size: 86 1132
AccI GT'mk_AC
Cuts at: 0 641 1218
Size: 641 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1181 1218
Size: 481 700 37
Fragments arranged by size:
700 481 37
AlwNI CAG_nnn'CTG
Cuts at: 0 717 1218
Size: 717 501
ApaI G_GGCC'C
Cuts at: 0 1159 1218
Size: 1159 59
AvaI C'yCGr_G
Cuts at: 0 466 763 1218
Size: 466 297 455
Fragments arranged by size:
466 455 297
BamHI G'GATC_C
Cuts at: 0 822 1218
Size: 822 396
BanI G'GyrC_C
Cuts at: 0 701 1040 1218
Size: 701 339 178
Fragments arranged by size:
701 339 178
BanII G_rGCy'C
Cuts at: 0 106 1159 1218
Size: 106 1053 59
Fragments arranged by size:
1053 106 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 1218
Size: 287 931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 621 655 1039 1073 1218
Size: 621 34 384 34 145
Fragments arranged by size:
621 384 145 34 34
BclI T'GATC_A
Cuts at: 0 731 1218
Size: 731 487
BglI GCCn_nnn'nGGC
Cuts at: 0 1137 1218
Size: 1137 81
BglII A'GATC_T
Cuts at: 0 456 1218
Size: 456 762
BlpI GC'TnA_GC
Cuts at: 0 176 845 1218
Size: 176 669 373
Fragments arranged by size:
669 373 176
Bme1580I G_kGCm'C
Cuts at: 0 1079 1159 1218
Size: 1079 80 59
Fragments arranged by size:
1079 80 59
BmgBI CAC'GTC
Cuts at: 0 12 1218
Size: 12 1206
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 1089 1218
Size: 48 1041 129
Fragments arranged by size:
1041 129 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1191 1218
Size: 1191 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 789 1218
Size: 220 569 429
Fragments arranged by size:
569 429 220
Bpu10I CC'TnA_GC
Cuts at: 0 191 967 1218
Size: 191 776 251
Fragments arranged by size:
776 251 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 1218
Size: 410 808
BsaHI Gr'CG_yC
Cuts at: 0 83 1218
Size: 83 1135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 787 817 1218
Size: 730 30 27 30 401
Fragments arranged by size:
730 401 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 217 1180 1218
Size: 110 107 963 38
Fragments arranged by size:
963 110 107 38
BseYI C'CCAG_C
Cuts at: 0 1212 1218
Size: 1212 6
BsiHKAI G_wGCw'C
Cuts at: 0 106 1218
Size: 106 1112
BsmI GAATG_Cn'
Cuts at: 0 1005 1218
Size: 1005 213
Bsp1286I G_dGCh'C
Cuts at: 0 106 1079 1159 1218
Size: 106 973 80 59
Fragments arranged by size:
973 106 80 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 807 1218
Size: 807 411
BsrBI CCG'CTC
Cuts at: 0 180 1218
Size: 180 1038
BsrFI r'CCGG_y
Cuts at: 0 253 704 1127 1218
Size: 253 451 423 91
Fragments arranged by size:
451 423 253 91
BssSI C'ACGA_G
Cuts at: 0 142 1218
Size: 142 1076
BstXI CCAn_nnnn'nTGG
Cuts at: 0 698 1218
Size: 698 520
BstYI r'GATC_y
Cuts at: 0 456 497 590 713 822 1218
Size: 456 41 93 123 109 396
Fragments arranged by size:
456 396 123 109 93 41
Bsu36I CC'TnA_GG
Cuts at: 0 77 827 1218
Size: 77 750 391
Fragments arranged by size:
750 391 77
BtgI C'CryG_G
Cuts at: 0 605 1218
Size: 605 613
BtsI GCAGTG_nn'
Cuts at: 0 425 938 1218
Size: 425 513 280
Fragments arranged by size:
513 425 280
ClaI AT'CG_AT
Cuts at: 0 281 1218
Size: 281 937
EaeI y'GGCC_r
Cuts at: 0 138 255 985 1218
Size: 138 117 730 233
Fragments arranged by size:
730 233 138 117
EarI CTCTTCn'nnn_
Cuts at: 0 1157 1218
Size: 1157 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 1218
Size: 103 1115
EcoICRI GAG'CTC
Cuts at: 0 104 1218
Size: 104 1114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 481 789 1181 1218
Size: 220 261 308 392 37
Fragments arranged by size:
392 308 261 220 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 1218
Size: 244 974
EcoO109I rG'GnC_Cy
Cuts at: 0 566 852 1014 1156 1218
Size: 566 286 162 142 62
Fragments arranged by size:
566 286 162 142 62
HaeII r_GCGC'y
Cuts at: 0 586 1218
Size: 586 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 1218
Size: 787 32 399
Fragments arranged by size:
787 399 32
HincII GTy'rAC
Cuts at: 0 642 1218
Size: 642 576
HindIII A'AGCT_T
Cuts at: 0 547 1218
Size: 547 671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1218
Size: 525 693
MscI TGG'CCA
Cuts at: 0 140 1218
Size: 140 1078
MslI CAynn'nnrTG
Cuts at: 0 733 1218
Size: 733 485
MspA1I CmG'CkG
Cuts at: 0 175 198 844 918 1218
Size: 175 23 646 74 300
Fragments arranged by size:
646 300 175 74 23
NaeI GCC'GGC
Cuts at: 0 255 1218
Size: 255 963
NcoI C'CATG_G
Cuts at: 0 605 1218
Size: 605 613
NgoMIV G'CCGG_C
Cuts at: 0 253 1218
Size: 253 965
PasI CC'CwG_GG
Cuts at: 0 571 1135 1218
Size: 571 564 83
Fragments arranged by size:
571 564 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 628 1218
Size: 628 590
PpuMI rG'GwC_Cy
Cuts at: 0 566 1014 1218
Size: 566 448 204
Fragments arranged by size:
566 448 204
PspOMI G'GGCC_C
Cuts at: 0 1155 1218
Size: 1155 63
PstI C_TGCA'G
Cuts at: 0 818 1218
Size: 818 400
PvuII CAG'CTG
Cuts at: 0 175 198 844 1218
Size: 175 23 646 374
Fragments arranged by size:
646 374 175 23
SacI G_AGCT'C
Cuts at: 0 106 1218
Size: 106 1112
SalI G'TCGA_C
Cuts at: 0 640 1218
Size: 640 578
SanDI GG'GwC_CC
Cuts at: 0 566 1014 1218
Size: 566 448 204
Fragments arranged by size:
566 448 204
SexAI A'CCwGG_T
Cuts at: 0 388 1218
Size: 388 830
SfcI C'TryA_G
Cuts at: 0 814 856 1218
Size: 814 42 362
Fragments arranged by size:
814 362 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1137 1218
Size: 1137 81
SgrAI Cr'CCGG_yG
Cuts at: 0 704 1218
Size: 704 514
SspI AAT'ATT
Cuts at: 0 474 1218
Size: 474 744
StyI C'CwwG_G
Cuts at: 0 605 1218
Size: 605 613
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 635 988 1218
Size: 635 353 230
Fragments arranged by size:
635 353 230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 535 625 1218
Size: 535 90 593
Fragments arranged by size:
593 535 90
ZraI GAC'GTC
Cuts at: 0 84 1218
Size: 84 1134
Enzymes that do cut:
AarI AatII AccI AcuI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PasI PflMI PpuMI PspOMI PstI PvuII
SacI SalI SanDI SexAI SfcI SfiI SgrAI SspI
StyI TaqII XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BfrBI BmtI BpuEI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SapI SbfI ScaI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI