Restriction map for
pF1KE0701
(Linear) (Six-base) MAPSORT of: KIEE0701.seq Check: 3296 from: 1 to: 861
KIEE0701.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 231 861
Size: 231 630
AccI GT'mk_AC
Cuts at: 0 356 861
Size: 356 505
AclI AA'CG_TT
Cuts at: 0 231 861
Size: 231 630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 861
Size: 824 37
AhdI GACnn_n'nnGTC
Cuts at: 0 834 861
Size: 834 27
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 861
Size: 40 32 789
Fragments arranged by size:
789 40 32
AlwNI CAG_nnn'CTG
Cuts at: 0 825 861
Size: 825 36
ApoI r'AATT_y
Cuts at: 0 151 340 427 861
Size: 151 189 87 434
Fragments arranged by size:
434 189 151 87
AseI AT'TA_AT
Cuts at: 0 194 861
Size: 194 667
BamHI G'GATC_C
Cuts at: 0 125 861
Size: 125 736
BanII G_rGCy'C
Cuts at: 0 459 861
Size: 459 402
BbsI GAAGACnn'nnnn_
Cuts at: 0 731 830 861
Size: 731 99 31
Fragments arranged by size:
731 99 31
BfrBI ATG'CAT
Cuts at: 0 718 861
Size: 718 143
BglI GCCn_nnn'nGGC
Cuts at: 0 504 861
Size: 504 357
BlpI GC'TnA_GC
Cuts at: 0 508 861
Size: 508 353
BmgBI CAC'GTC
Cuts at: 0 318 861
Size: 318 543
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 496 657 861
Size: 131 365 161 204
Fragments arranged by size:
365 204 161 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 861
Size: 42 819
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 861
Size: 76 785
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 365 395 861
Size: 40 30 295 30 466
Fragments arranged by size:
466 295 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 861
Size: 799 62
BseYI C'CCAG_C
Cuts at: 0 129 861
Size: 129 732
BsiHKAI G_wGCw'C
Cuts at: 0 685 861
Size: 685 176
Bsp1286I G_dGCh'C
Cuts at: 0 459 685 861
Size: 459 226 176
Fragments arranged by size:
459 226 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 861
Size: 231 630
BsrGI T'GTAC_A
Cuts at: 0 563 861
Size: 563 298
BstYI r'GATC_y
Cuts at: 0 125 845 861
Size: 125 720 16
Fragments arranged by size:
720 125 16
BtgI C'CryG_G
Cuts at: 0 74 861
Size: 74 787
BtsI GCAGTG_nn'
Cuts at: 0 485 861
Size: 485 376
DraI TTT'AAA
Cuts at: 0 237 861
Size: 237 624
DrdI GACnn_nn'nnGTC
Cuts at: 0 402 861
Size: 402 459
EaeI y'GGCC_r
Cuts at: 0 5 164 861
Size: 5 159 697
Fragments arranged by size:
697 159 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 824 861
Size: 42 782 37
Fragments arranged by size:
782 42 37
EcoO109I rG'GnC_Cy
Cuts at: 0 303 861
Size: 303 558
EcoRI G'AATT_C
Cuts at: 0 340 861
Size: 340 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 795 820 827 852 861
Size: 795 25 7 25 9
Fragments arranged by size:
795 25 25 9 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 861
Size: 687 174
MscI TGG'CCA
Cuts at: 0 166 861
Size: 166 695
MspA1I CmG'CkG
Cuts at: 0 133 172 741 861
Size: 133 39 569 120
Fragments arranged by size:
569 133 120 39
NcoI C'CATG_G
Cuts at: 0 74 861
Size: 74 787
NdeI CA'TA_TG
Cuts at: 0 696 861
Size: 696 165
NsiI A_TGCA'T
Cuts at: 0 720 861
Size: 720 141
NspI r_CATG'y
Cuts at: 0 333 861
Size: 333 528
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 861
Size: 14 847
PfoI T'CCnGG_A
Cuts at: 0 210 861
Size: 210 651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 861
Size: 40 32 789
Fragments arranged by size:
789 40 32
PpuMI rG'GwC_Cy
Cuts at: 0 303 861
Size: 303 558
PshAI GACnn'nnGTC
Cuts at: 0 489 861
Size: 489 372
PvuII CAG'CTG
Cuts at: 0 133 172 741 861
Size: 133 39 569 120
Fragments arranged by size:
569 133 120 39
SfcI C'TryA_G
Cuts at: 0 645 651 861
Size: 645 6 210
Fragments arranged by size:
645 210 6
SphI G_CATG'C
Cuts at: 0 333 861
Size: 333 528
StyI C'CwwG_G
Cuts at: 0 74 861
Size: 74 787
TatI w'GTAC_w
Cuts at: 0 563 861
Size: 563 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 861
Size: 365 32 464
Fragments arranged by size:
464 365 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 738 861
Size: 15 723 123
Fragments arranged by size:
723 123 15
Enzymes that do cut:
AarI AccI AclI AcuI AhdI AloI AlwNI ApoI
AseI BamHI BanII BbsI BfrBI BglI BlpI BmgBI
BmrI BpmI BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspMI BsrGI BstYI BtgI BtsI DraI DrdI EaeI
Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MspA1I NcoI
NdeI NsiI NspI PflMI PfoI PpiI PpuMI PshAI
PvuII SfcI SphI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AleI AlfI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BglII Bme1580I BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII EagI EarI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI