Restriction map for
pF1KE0696
(Linear) (Six-base) MAPSORT of: KIEE0696.seq Check: 4508 from: 1 to: 1212
KIEE0696.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 1175 1212
Size: 1175 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 902 1212
Size: 842 60 310
Fragments arranged by size:
842 310 60
AflIII A'CryG_T
Cuts at: 0 162 1212
Size: 162 1050
AlwNI CAG_nnn'CTG
Cuts at: 0 853 1212
Size: 853 359
ApoI r'AATT_y
Cuts at: 0 70 1212
Size: 70 1142
BanI G'GyrC_C
Cuts at: 0 856 1212
Size: 856 356
BbeI G_GCGC'C
Cuts at: 0 860 1212
Size: 860 352
BbsI GAAGACnn'nnnn_
Cuts at: 0 887 1212
Size: 887 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1024 1058 1212
Size: 1024 34 154
Fragments arranged by size:
1024 154 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 90 1212
Size: 90 1122
BclI T'GATC_A
Cuts at: 0 285 1212
Size: 285 927
BglII A'GATC_T
Cuts at: 0 886 1212
Size: 886 326
BmgBI CAC'GTC
Cuts at: 0 1209 1212
Size: 1209 3
Bpu10I CC'TnA_GC
Cuts at: 0 217 1212
Size: 217 995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1212
Size: 545 667
BsaI GGTCTCn'nnnn_
Cuts at: 0 1005 1212
Size: 1005 207
BsaAI yAC'GTr
Cuts at: 0 163 1212
Size: 163 1049
BsaHI Gr'CG_yC
Cuts at: 0 857 1212
Size: 857 355
BsaWI w'CCGG_w
Cuts at: 0 1197 1212
Size: 1197 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1068 1098 1212
Size: 1068 30 114
Fragments arranged by size:
1068 114 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1212
Size: 826 386
BsmI GAATG_Cn'
Cuts at: 0 255 1212
Size: 255 957
BsmBI CGTCTCn'nnnn_
Cuts at: 0 794 1212
Size: 794 418
BstBI TT'CG_AA
Cuts at: 0 78 1212
Size: 78 1134
BstYI r'GATC_y
Cuts at: 0 886 1212
Size: 886 326
Bsu36I CC'TnA_GG
Cuts at: 0 1151 1212
Size: 1151 61
BtgI C'CryG_G
Cuts at: 0 390 1013 1212
Size: 390 623 199
Fragments arranged by size:
623 390 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 218 1212
Size: 218 994
BtsI GCAGTG_nn'
Cuts at: 0 614 1212
Size: 614 598
EarI CTCTTCn'nnn_
Cuts at: 0 619 698 818 1212
Size: 619 79 120 394
Fragments arranged by size:
619 394 120 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1212
Size: 244 968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 902 1212
Size: 842 60 310
Fragments arranged by size:
842 310 60
EcoO109I rG'GnC_Cy
Cuts at: 0 294 877 1212
Size: 294 583 335
Fragments arranged by size:
583 335 294
HaeII r_GCGC'y
Cuts at: 0 860 1212
Size: 860 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1212
Size: 1068 32 112
Fragments arranged by size:
1068 112 32
HindIII A'AGCT_T
Cuts at: 0 178 1212
Size: 178 1034
KasI G'GCGC_C
Cuts at: 0 856 1212
Size: 856 356
MfeI C'AATT_G
Cuts at: 0 640 1212
Size: 640 572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 817 1212
Size: 147 670 395
Fragments arranged by size:
670 395 147
MslI CAynn'nnrTG
Cuts at: 0 754 1212
Size: 754 458
MspA1I CmG'CkG
Cuts at: 0 873 1212
Size: 873 339
NarI GG'CG_CC
Cuts at: 0 857 1212
Size: 857 355
NcoI C'CATG_G
Cuts at: 0 390 1013 1212
Size: 390 623 199
Fragments arranged by size:
623 390 199
PfoI T'CCnGG_A
Cuts at: 0 728 1212
Size: 728 484
PmlI CAC'GTG
Cuts at: 0 163 1212
Size: 163 1049
PpuMI rG'GwC_Cy
Cuts at: 0 294 877 1212
Size: 294 583 335
Fragments arranged by size:
583 335 294
SanDI GG'GwC_CC
Cuts at: 0 877 1212
Size: 877 335
ScaI AGT'ACT
Cuts at: 0 268 1212
Size: 268 944
SfoI GGC'GCC
Cuts at: 0 858 1212
Size: 858 354
SmlI C'TyrA_G
Cuts at: 0 560 1212
Size: 560 652
StyI C'CwwG_G
Cuts at: 0 390 1013 1046 1212
Size: 390 623 33 166
Fragments arranged by size:
623 390 166 33
TatI w'GTAC_w
Cuts at: 0 266 1212
Size: 266 946
XbaI T'CTAG_A
Cuts at: 0 39 1212
Size: 39 1173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 1212
Size: 397 815
XmnI GAAnn'nnTTC
Cuts at: 0 268 692 1212
Size: 268 424 520
Fragments arranged by size:
520 424 268
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI BanI BbeI BbsI
BcgI BciVI BclI BglII BmgBI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BsgI BsmI BsmBI BstBI
BstYI Bsu36I BtgI BtgZI BtsI EarI EciI Eco57MI
EcoO109I HaeII Hin4I HindIII KasI MfeI MmeI MslI
MspA1I NarI NcoI PfoI PmlI PpuMI SanDI ScaI
SfoI SmlI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbvCI BfrBI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI BsaBI BseRI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KpnI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XhoI XmaI ZraI