Restriction map for
pF1KE0694
(Linear) (Six-base) MAPSORT of: KIEE0694.seq Check: 8649 from: 1 to: 1209
KIEE0694.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 763 1209
Size: 763 446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 881 1209
Size: 386 495 328
Fragments arranged by size:
495 386 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 434 1209
Size: 434 775
ApaI G_GGCC'C
Cuts at: 0 82 1209
Size: 82 1127
ApoI r'AATT_y
Cuts at: 0 47 258 869 995 1209
Size: 47 211 611 126 214
Fragments arranged by size:
611 214 211 126 47
BanI G'GyrC_C
Cuts at: 0 247 1209
Size: 247 962
BanII G_rGCy'C
Cuts at: 0 82 1209
Size: 82 1127
BbsI GAAGACnn'nnnn_
Cuts at: 0 629 1209
Size: 629 580
BbvCI CC'TCA_GC
Cuts at: 0 668 687 1209
Size: 668 19 522
Fragments arranged by size:
668 522 19
BfrBI ATG'CAT
Cuts at: 0 415 1209
Size: 415 794
BglI GCCn_nnn'nGGC
Cuts at: 0 956 1209
Size: 956 253
BglII A'GATC_T
Cuts at: 0 1203 1209
Size: 1203 6
Bme1580I G_kGCm'C
Cuts at: 0 82 155 1209
Size: 82 73 1054
Fragments arranged by size:
1054 82 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 338 807 1209
Size: 338 469 402
Fragments arranged by size:
469 402 338
BmtI G_CTAG'C
Cuts at: 0 950 1209
Size: 950 259
Bpu10I CC'TnA_GC
Cuts at: 0 668 687 1209
Size: 668 19 522
Fragments arranged by size:
668 522 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 1209
Size: 31 1178
BsaHI Gr'CG_yC
Cuts at: 0 4 1209
Size: 4 1205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1079 1109 1209
Size: 1079 30 100
Fragments arranged by size:
1079 100 30
BseYI C'CCAG_C
Cuts at: 0 8 306 1209
Size: 8 298 903
Fragments arranged by size:
903 298 8
Bsp1286I G_dGCh'C
Cuts at: 0 82 155 1209
Size: 82 73 1054
Fragments arranged by size:
1054 82 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 225 987 1209
Size: 225 762 222
Fragments arranged by size:
762 225 222
BstYI r'GATC_y
Cuts at: 0 1203 1209
Size: 1203 6
BtgI C'CryG_G
Cuts at: 0 1091 1209
Size: 1091 118
BtsI GCAGTG_nn'
Cuts at: 0 204 1209
Size: 204 1005
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 912 947 1209
Size: 912 35 262
Fragments arranged by size:
912 262 35
EaeI y'GGCC_r
Cuts at: 0 700 957 1209
Size: 700 257 252
Fragments arranged by size:
700 257 252
EarI CTCTTCn'nnn_
Cuts at: 0 600 1209
Size: 600 609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 881 1209
Size: 386 495 328
Fragments arranged by size:
495 386 328
EcoO109I rG'GnC_Cy
Cuts at: 0 79 106 1209
Size: 79 27 1103
Fragments arranged by size:
1103 79 27
EcoRI G'AATT_C
Cuts at: 0 47 1209
Size: 47 1162
MscI TGG'CCA
Cuts at: 0 702 959 1209
Size: 702 257 250
Fragments arranged by size:
702 257 250
MslI CAynn'nnrTG
Cuts at: 0 773 1209
Size: 773 436
MspA1I CmG'CkG
Cuts at: 0 457 588 1209
Size: 457 131 621
Fragments arranged by size:
621 457 131
NheI G'CTAG_C
Cuts at: 0 946 1209
Size: 946 263
NsiI A_TGCA'T
Cuts at: 0 417 1209
Size: 417 792
NspI r_CATG'y
Cuts at: 0 415 659 1209
Size: 415 244 550
Fragments arranged by size:
550 415 244
PasI CC'CwG_GG
Cuts at: 0 32 1209
Size: 32 1177
PflMI CCAn_nnn'nTGG
Cuts at: 0 32 1209
Size: 32 1177
PfoI T'CCnGG_A
Cuts at: 0 51 1209
Size: 51 1158
PpuMI rG'GwC_Cy
Cuts at: 0 106 1209
Size: 106 1103
PspOMI G'GGCC_C
Cuts at: 0 78 1209
Size: 78 1131
PvuII CAG'CTG
Cuts at: 0 457 1209
Size: 457 752
RsrII CG'GwC_CG
Cuts at: 0 61 1209
Size: 61 1148
SfcI C'TryA_G
Cuts at: 0 276 583 1209
Size: 276 307 626
Fragments arranged by size:
626 307 276
SphI G_CATG'C
Cuts at: 0 415 659 1209
Size: 415 244 550
Fragments arranged by size:
550 415 244
SspI AAT'ATT
Cuts at: 0 707 1209
Size: 707 502
StyI C'CwwG_G
Cuts at: 0 169 660 729 1209
Size: 169 491 69 480
Fragments arranged by size:
491 480 169 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 357 689 1209
Size: 357 332 520
Fragments arranged by size:
520 357 332
Enzymes that do cut:
AccI AcuI AlfI ApaI ApoI BanI BanII BbsI
BbvCI BfrBI BglI BglII Bme1580I BmrI BmtI Bpu10I
BsaI BsaHI BsaXI BseYI Bsp1286I BspMI BstYI BtgI
BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRI MscI
MslI MspA1I NheI NsiI NspI PasI PflMI PfoI
PpuMI PspOMI PvuII RsrII SfcI SphI SspI StyI
TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BlpI BmgBI BplI BpmI BpuEI BsaAI BsaBI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NotI NruI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI