Restriction map for
pF1KE0692
(Linear) (Six-base) MAPSORT of: KIEE0692.seq Check: 9204 from: 1 to: 1206
KIEE0692.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 0 280 1206
Size: 280 926
AccI GT'mk_AC
Cuts at: 0 596 1206
Size: 596 610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1145 1206
Size: 674 471 61
Fragments arranged by size:
674 471 61
ApaLI G'TGCA_C
Cuts at: 0 447 1206
Size: 447 759
ApoI r'AATT_y
Cuts at: 0 883 977 1206
Size: 883 94 229
Fragments arranged by size:
883 229 94
BanI G'GyrC_C
Cuts at: 0 61 462 616 1206
Size: 61 401 154 590
Fragments arranged by size:
590 401 154 61
BbeI G_GCGC'C
Cuts at: 0 65 1206
Size: 65 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 206 965 1206
Size: 206 759 241
Fragments arranged by size:
759 241 206
BglII A'GATC_T
Cuts at: 0 693 1109 1206
Size: 693 416 97
Fragments arranged by size:
693 416 97
BlpI GC'TnA_GC
Cuts at: 0 398 1206
Size: 398 808
Bme1580I G_kGCm'C
Cuts at: 0 451 1206
Size: 451 755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 231 248 1206
Size: 97 134 17 958
Fragments arranged by size:
958 134 97 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 595 1206
Size: 595 611
BsaBI GATnn'nnATC
Cuts at: 0 433 1206
Size: 433 773
BsaHI Gr'CG_yC
Cuts at: 0 62 277 1206
Size: 62 215 929
Fragments arranged by size:
929 215 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 150 180 499 529 1206
Size: 150 30 319 30 677
Fragments arranged by size:
677 319 150 30 30
BseYI C'CCAG_C
Cuts at: 0 241 467 1206
Size: 241 226 739
Fragments arranged by size:
739 241 226
BsiHKAI G_wGCw'C
Cuts at: 0 451 1206
Size: 451 755
Bsp1286I G_dGCh'C
Cuts at: 0 451 1206
Size: 451 755
BspHI T'CATG_A
Cuts at: 0 330 531 1206
Size: 330 201 675
Fragments arranged by size:
675 330 201
BsrDI GCAATG_nn'
Cuts at: 0 566 1206
Size: 566 640
BsrGI T'GTAC_A
Cuts at: 0 878 1206
Size: 878 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 1206
Size: 408 798
BstYI r'GATC_y
Cuts at: 0 693 804 832 1109 1206
Size: 693 111 28 277 97
Fragments arranged by size:
693 277 111 97 28
Bsu36I CC'TnA_GG
Cuts at: 0 710 745 1206
Size: 710 35 461
Fragments arranged by size:
710 461 35
BtsI GCAGTG_nn'
Cuts at: 0 663 1206
Size: 663 543
DraIII CAC_nnn'GTG
Cuts at: 0 225 1206
Size: 225 981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 231 248 674 1145 1206
Size: 97 134 17 426 471 61
Fragments arranged by size:
471 426 134 97 61 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 263 1206
Size: 263 943
HaeII r_GCGC'y
Cuts at: 0 65 1206
Size: 65 1141
KasI G'GCGC_C
Cuts at: 0 61 1206
Size: 61 1145
MslI CAynn'nnrTG
Cuts at: 0 308 382 1206
Size: 308 74 824
Fragments arranged by size:
824 308 74
MspA1I CmG'CkG
Cuts at: 0 471 1206
Size: 471 735
NarI GG'CG_CC
Cuts at: 0 62 1206
Size: 62 1144
PflMI CCAn_nnn'nTGG
Cuts at: 0 784 1206
Size: 784 422
PfoI T'CCnGG_A
Cuts at: 0 264 1206
Size: 264 942
SfcI C'TryA_G
Cuts at: 0 510 1206
Size: 510 696
SfoI GGC'GCC
Cuts at: 0 63 1206
Size: 63 1143
StuI AGG'CCT
Cuts at: 0 1175 1206
Size: 1175 31
StyI C'CwwG_G
Cuts at: 0 47 1206
Size: 47 1159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1058 1206
Size: 1058 148
TatI w'GTAC_w
Cuts at: 0 878 1191 1206
Size: 878 313 15
Fragments arranged by size:
878 313 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 150 182 1206
Size: 150 32 1024
Fragments arranged by size:
1024 150 32
Tth111I GACn'n_nGTC
Cuts at: 0 592 1206
Size: 592 614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 1206
Size: 54 1152
ZraI GAC'GTC
Cuts at: 0 278 1206
Size: 278 928
Enzymes that do cut:
AatII AccI AcuI ApaLI ApoI BanI BbeI BbsI
BglII BlpI Bme1580I BpmI BsaI BsaBI BsaHI BsaXI
BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrGI BstAPI BstYI
Bsu36I BtsI DraIII Eco57MI EcoNI HaeII KasI MslI
MspA1I NarI PflMI PfoI SfcI SfoI StuI StyI
TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbvCI BcgI BciVI
BclI BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaWI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmaI XmnI