Restriction map for
pF1KE0691
(Linear) (Six-base) MAPSORT of: KIEE0691.seq Check: 2620 from: 1 to: 843
KIEE0691.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 0 654 843
Size: 654 189
AgeI A'CCGG_T
Cuts at: 0 611 843
Size: 611 232
AlwNI CAG_nnn'CTG
Cuts at: 0 88 843
Size: 88 755
AvaI C'yCGr_G
Cuts at: 0 20 28 393 843
Size: 20 8 365 450
Fragments arranged by size:
450 365 20 8
BanI G'GyrC_C
Cuts at: 0 12 420 843
Size: 12 408 423
Fragments arranged by size:
423 408 12
BanII G_rGCy'C
Cuts at: 0 500 843
Size: 500 343
BbeI G_GCGC'C
Cuts at: 0 16 843
Size: 16 827
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 843
Size: 253 590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 88 843
Size: 54 34 755
Fragments arranged by size:
755 54 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 695 843
Size: 695 148
BglI GCCn_nnn'nGGC
Cuts at: 0 10 151 843
Size: 10 141 692
Fragments arranged by size:
692 141 10
Bme1580I G_kGCm'C
Cuts at: 0 425 670 843
Size: 425 245 173
Fragments arranged by size:
425 245 173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 114 843
Size: 114 729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 295 358 484 499 808 843
Size: 220 75 63 126 15 309 35
Fragments arranged by size:
309 220 126 75 63 35 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 543 843
Size: 543 300
BsaAI yAC'GTr
Cuts at: 0 510 843
Size: 510 333
BsaHI Gr'CG_yC
Cuts at: 0 13 651 843
Size: 13 638 192
Fragments arranged by size:
638 192 13
BsaWI w'CCGG_w
Cuts at: 0 611 843
Size: 611 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 268 298 843
Size: 94 30 144 30 545
Fragments arranged by size:
545 144 94 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 739 843
Size: 610 129 104
Fragments arranged by size:
610 129 104
BseYI C'CCAG_C
Cuts at: 0 77 294 783 843
Size: 77 217 489 60
Fragments arranged by size:
489 217 77 60
BsiHKAI G_wGCw'C
Cuts at: 0 500 695 843
Size: 500 195 148
Fragments arranged by size:
500 195 148
BsmBI CGTCTCn'nnnn_
Cuts at: 0 523 843
Size: 523 320
Bsp1286I G_dGCh'C
Cuts at: 0 425 500 670 695 843
Size: 425 75 170 25 148
Fragments arranged by size:
425 170 148 75 25
BsrDI GCAATG_nn'
Cuts at: 0 808 843
Size: 808 35
BsrFI r'CCGG_y
Cuts at: 0 611 843
Size: 611 232
BstYI r'GATC_y
Cuts at: 0 377 843
Size: 377 466
BtgI C'CryG_G
Cuts at: 0 113 843
Size: 113 730
DraIII CAC_nnn'GTG
Cuts at: 0 128 843
Size: 128 715
EaeI y'GGCC_r
Cuts at: 0 2 140 233 443 843
Size: 2 138 93 210 400
Fragments arranged by size:
400 210 138 93 2
EcoICRI GAG'CTC
Cuts at: 0 498 843
Size: 498 345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 295 358 484 499 808 843
Size: 220 75 63 126 15 309 35
Fragments arranged by size:
309 220 126 75 63 35 15
EcoO109I rG'GnC_Cy
Cuts at: 0 24 299 411 744 843
Size: 24 275 112 333 99
Fragments arranged by size:
333 275 112 99 24
FspI TGC'GCA
Cuts at: 0 361 843
Size: 361 482
HaeII r_GCGC'y
Cuts at: 0 16 843
Size: 16 827
KasI G'GCGC_C
Cuts at: 0 12 843
Size: 12 831
MspA1I CmG'CkG
Cuts at: 0 85 253 273 843
Size: 85 168 20 570
Fragments arranged by size:
570 168 85 20
NarI GG'CG_CC
Cuts at: 0 13 843
Size: 13 830
PmlI CAC'GTG
Cuts at: 0 510 843
Size: 510 333
PpuMI rG'GwC_Cy
Cuts at: 0 24 299 843
Size: 24 275 544
Fragments arranged by size:
544 275 24
PshAI GACnn'nnGTC
Cuts at: 0 56 535 843
Size: 56 479 308
Fragments arranged by size:
479 308 56
PstI C_TGCA'G
Cuts at: 0 311 329 843
Size: 311 18 514
Fragments arranged by size:
514 311 18
PvuII CAG'CTG
Cuts at: 0 85 253 273 843
Size: 85 168 20 570
Fragments arranged by size:
570 168 85 20
SacI G_AGCT'C
Cuts at: 0 500 843
Size: 500 343
SanDI GG'GwC_CC
Cuts at: 0 24 843
Size: 24 819
SfcI C'TryA_G
Cuts at: 0 307 325 843
Size: 307 18 518
Fragments arranged by size:
518 307 18
SfoI GGC'GCC
Cuts at: 0 14 843
Size: 14 829
SmaI CCC'GGG
Cuts at: 0 22 395 843
Size: 22 373 448
Fragments arranged by size:
448 373 22
StuI AGG'CCT
Cuts at: 0 154 843
Size: 154 689
StyI C'CwwG_G
Cuts at: 0 155 416 655 843
Size: 155 261 239 188
Fragments arranged by size:
261 239 188 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 843
Size: 268 32 543
Fragments arranged by size:
543 268 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 843
Size: 153 690
XmaI C'CCGG_G
Cuts at: 0 20 393 843
Size: 20 373 450
Fragments arranged by size:
450 373 20
ZraI GAC'GTC
Cuts at: 0 652 843
Size: 652 191
Enzymes that do cut:
AatII AgeI AlwNI AvaI BanI BanII BbeI BbsI
BcgI BciVI BglI Bme1580I BplI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BstYI BtgI DraIII EaeI EcoICRI Eco57MI
EcoO109I FspI HaeII KasI MspA1I NarI PmlI PpuMI
PshAI PstI PvuII SacI SanDI SfcI SfoI SmaI
StuI StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaBI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI