Restriction map for
pF1KE0689
(Linear) (Six-base) MAPSORT of: KIEE0689.seq Check: 9233 from: 1 to: 840
KIEE0689.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 840
Size: 155 685
AhdI GACnn_n'nnGTC
Cuts at: 0 597 840
Size: 597 243
ApoI r'AATT_y
Cuts at: 0 277 292 840
Size: 277 15 548
Fragments arranged by size:
548 277 15
BamHI G'GATC_C
Cuts at: 0 42 617 840
Size: 42 575 223
Fragments arranged by size:
575 223 42
BanI G'GyrC_C
Cuts at: 0 493 840
Size: 493 347
BglII A'GATC_T
Cuts at: 0 51 840
Size: 51 789
BlpI GC'TnA_GC
Cuts at: 0 80 840
Size: 80 760
Bme1580I G_kGCm'C
Cuts at: 0 476 496 840
Size: 476 20 344
Fragments arranged by size:
476 344 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 840
Size: 73 767
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 840
Size: 187 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 515 545 840
Size: 515 30 295
Fragments arranged by size:
515 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 840
Size: 136 704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 840
Size: 547 293
BsiEI CG_ry'CG
Cuts at: 0 826 840
Size: 826 14
BsiHKAI G_wGCw'C
Cuts at: 0 37 840
Size: 37 803
Bsp1286I G_dGCh'C
Cuts at: 0 37 476 496 840
Size: 37 439 20 344
Fragments arranged by size:
439 344 37 20
BsrDI GCAATG_nn'
Cuts at: 0 195 446 840
Size: 195 251 394
Fragments arranged by size:
394 251 195
BsrGI T'GTAC_A
Cuts at: 0 645 840
Size: 645 195
BstEII G'GTnAC_C
Cuts at: 0 505 840
Size: 505 335
BstYI r'GATC_y
Cuts at: 0 42 51 64 617 840
Size: 42 9 13 553 223
Fragments arranged by size:
553 223 42 13 9
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 377 412 840
Size: 377 35 428
Fragments arranged by size:
428 377 35
DraI TTT'AAA
Cuts at: 0 276 837 840
Size: 276 561 3
Fragments arranged by size:
561 276 3
EarI CTCTTCn'nnn_
Cuts at: 0 146 840
Size: 146 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 155 840
Size: 73 82 685
Fragments arranged by size:
685 82 73
EcoRI G'AATT_C
Cuts at: 0 292 840
Size: 292 548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 484 840
Size: 484 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 515 547 583 615 696 728
Size: 345 32 138 32 36 32 81 32
Cuts at: 728 804 836 840
Size: 76 32 4
Fragments arranged by size:
345 138 81 76 36 32 32 32
32 32 4
MslI CAynn'nnrTG
Cuts at: 0 248 364 439 569 840
Size: 248 116 75 130 271
Fragments arranged by size:
271 248 130 116 75
MspA1I CmG'CkG
Cuts at: 0 643 840
Size: 643 197
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 840
Size: 96 744
PfoI T'CCnGG_A
Cuts at: 0 89 840
Size: 89 751
PvuII CAG'CTG
Cuts at: 0 643 840
Size: 643 197
SapI GCTCTTCn'nnn_
Cuts at: 0 146 840
Size: 146 694
StuI AGG'CCT
Cuts at: 0 527 840
Size: 527 313
StyI C'CwwG_G
Cuts at: 0 798 840
Size: 798 42
SwaI ATTT'AAAT
Cuts at: 0 276 840
Size: 276 564
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 812 840
Size: 52 760 28
Fragments arranged by size:
760 52 28
TatI w'GTAC_w
Cuts at: 0 206 645 840
Size: 206 439 195
Fragments arranged by size:
439 206 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 85 117 840
Size: 27 32 26 32 723
Fragments arranged by size:
723 32 32 27 26
XbaI T'CTAG_A
Cuts at: 0 18 840
Size: 18 822
XmnI GAAnn'nnTTC
Cuts at: 0 140 583 611 840
Size: 140 443 28 229
Fragments arranged by size:
443 229 140 28
Enzymes that do cut:
AcuI AhdI ApoI BamHI BanI BglII BlpI Bme1580I
BpmI BsaI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BsrDI BsrGI BstEII BstYI CspCI DraI EarI Eco57MI
EcoRI FalI Hin4I MslI MspA1I PflMI PfoI PvuII
SapI StuI StyI SwaI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI Tth111I XcmI
XhoI XmaI ZraI