Restriction map for
pF1KE0685
(Linear) (Six-base) MAPSORT of: KIEE0685.seq Check: 9331 from: 1 to: 840
KIEE0685.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 840
Size: 424 416
AflIII A'CryG_T
Cuts at: 0 521 840
Size: 521 319
AlwNI CAG_nnn'CTG
Cuts at: 0 484 840
Size: 484 356
ApaI G_GGCC'C
Cuts at: 0 412 819 840
Size: 412 407 21
Fragments arranged by size:
412 407 21
ApoI r'AATT_y
Cuts at: 0 826 840
Size: 826 14
BanI G'GyrC_C
Cuts at: 0 148 840
Size: 148 692
BanII G_rGCy'C
Cuts at: 0 298 412 819 840
Size: 298 114 407 21
Fragments arranged by size:
407 298 114 21
BbvCI CC'TCA_GC
Cuts at: 0 554 840
Size: 554 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 762 796 840
Size: 762 34 44
Fragments arranged by size:
762 44 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 58 840
Size: 58 782
BglI GCCn_nnn'nGGC
Cuts at: 0 62 840
Size: 62 778
Bme1580I G_kGCm'C
Cuts at: 0 200 412 491 819 840
Size: 200 212 79 328 21
Fragments arranged by size:
328 212 200 79 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 840
Size: 70 770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 840
Size: 697 143
Bpu10I CC'TnA_GC
Cuts at: 0 554 566 840
Size: 554 12 274
Fragments arranged by size:
554 274 12
BsaAI yAC'GTr
Cuts at: 0 360 840
Size: 360 480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 295 325 840
Size: 208 30 57 30 515
Fragments arranged by size:
515 208 57 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 592 595 613 799 840
Size: 229 363 3 18 186 41
Fragments arranged by size:
363 229 186 41 18 3
BseYI C'CCAG_C
Cuts at: 0 52 605 818 840
Size: 52 553 213 22
Fragments arranged by size:
553 213 52 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 840
Size: 192 648
BsiEI CG_ry'CG
Cuts at: 0 835 840
Size: 835 5
BsiHKAI G_wGCw'C
Cuts at: 0 132 644 840
Size: 132 512 196
Fragments arranged by size:
512 196 132
BsmI GAATG_Cn'
Cuts at: 0 452 840
Size: 452 388
BsmBI CGTCTCn'nnnn_
Cuts at: 0 681 840
Size: 681 159
Bsp1286I G_dGCh'C
Cuts at: 0 132 200 298 412 491 644 819 840
Size: 132 68 98 114 79 153 175 21
Fragments arranged by size:
175 153 132 114 98 79 68 21
BsrFI r'CCGG_y
Cuts at: 0 629 840
Size: 629 211
BssHII G'CGCG_C
Cuts at: 0 7 840
Size: 7 833
BstEII G'GTnAC_C
Cuts at: 0 615 840
Size: 615 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 840
Size: 60 780
Bsu36I CC'TnA_GG
Cuts at: 0 497 505 840
Size: 497 8 335
Fragments arranged by size:
497 335 8
DraIII CAC_nnn'GTG
Cuts at: 0 441 840
Size: 441 399
EarI CTCTTCn'nnn_
Cuts at: 0 166 439 840
Size: 166 273 401
Fragments arranged by size:
401 273 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 697 840
Size: 424 273 143
Fragments arranged by size:
424 273 143
EcoO109I rG'GnC_Cy
Cuts at: 0 33 408 409 501 588 840
Size: 33 375 1 92 87 252
Fragments arranged by size:
375 252 92 87 33 1
FspI TGC'GCA
Cuts at: 0 772 840
Size: 772 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 295 327 741 773 840
Size: 208 32 55 32 414 32 67
Fragments arranged by size:
414 208 67 55 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 526 840
Size: 526 314
MspA1I CmG'CkG
Cuts at: 0 391 768 840
Size: 391 377 72
Fragments arranged by size:
391 377 72
NaeI GCC'GGC
Cuts at: 0 631 840
Size: 631 209
NdeI CA'TA_TG
Cuts at: 0 537 840
Size: 537 303
NgoMIV G'CCGG_C
Cuts at: 0 629 840
Size: 629 211
PpuMI rG'GwC_Cy
Cuts at: 0 33 588 840
Size: 33 555 252
Fragments arranged by size:
555 252 33
PspOMI G'GGCC_C
Cuts at: 0 408 815 840
Size: 408 407 25
Fragments arranged by size:
408 407 25
PvuII CAG'CTG
Cuts at: 0 768 840
Size: 768 72
SanDI GG'GwC_CC
Cuts at: 0 33 588 840
Size: 33 555 252
Fragments arranged by size:
555 252 33
SfcI C'TryA_G
Cuts at: 0 260 840
Size: 260 580
StyI C'CwwG_G
Cuts at: 0 395 572 840
Size: 395 177 268
Fragments arranged by size:
395 268 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 840
Size: 597 243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 376 840
Size: 376 464
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI BanI BanII BbvCI
BcgI BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BsrFI BssHII BstEII BstXI Bsu36I DraIII EarI
Eco57MI EcoO109I FspI Hin4I MslI MspA1I NaeI NdeI
NgoMIV PpuMI PspOMI PvuII SanDI SfcI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsiWI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI