Restriction map for
pF1KE0683
(Linear) (Six-base) MAPSORT of: KIEE0683.seq Check: 5182 from: 1 to: 822
KIEE0683.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 784 822
Size: 340 444 38
Fragments arranged by size:
444 340 38
AleI CACnn'nnGTG
Cuts at: 0 55 822
Size: 55 767
AlwNI CAG_nnn'CTG
Cuts at: 0 318 822
Size: 318 504
ApaI G_GGCC'C
Cuts at: 0 105 822
Size: 105 717
AvaI C'yCGr_G
Cuts at: 0 53 104 822
Size: 53 51 718
Fragments arranged by size:
718 53 51
BanI G'GyrC_C
Cuts at: 0 179 411 533 822
Size: 179 232 122 289
Fragments arranged by size:
289 232 179 122
BanII G_rGCy'C
Cuts at: 0 105 774 822
Size: 105 669 48
Fragments arranged by size:
669 105 48
BciVI GTATCCnnnnn_n'
Cuts at: 0 432 822
Size: 432 390
BfrBI ATG'CAT
Cuts at: 0 678 822
Size: 678 144
BglI GCCn_nnn'nGGC
Cuts at: 0 246 822
Size: 246 576
BglII A'GATC_T
Cuts at: 0 150 567 822
Size: 150 417 255
Fragments arranged by size:
417 255 150
Bme1580I G_kGCm'C
Cuts at: 0 105 182 416 764 822
Size: 105 77 234 348 58
Fragments arranged by size:
348 234 105 77 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 211 822
Size: 211 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 758 822
Size: 349 409 64
Fragments arranged by size:
409 349 64
BsaAI yAC'GTr
Cuts at: 0 759 822
Size: 759 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 822
Size: 475 347
BseYI C'CCAG_C
Cuts at: 0 35 822
Size: 35 787
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 822
Size: 175 647
BsiHKAI G_wGCw'C
Cuts at: 0 774 822
Size: 774 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 625 822
Size: 625 197
Bsp1286I G_dGCh'C
Cuts at: 0 105 182 416 764 774 822
Size: 105 77 234 348 10 48
Fragments arranged by size:
348 234 105 77 48 10
BsrGI T'GTAC_A
Cuts at: 0 498 822
Size: 498 324
BssSI C'ACGA_G
Cuts at: 0 334 822
Size: 334 488
BstYI r'GATC_y
Cuts at: 0 150 567 822
Size: 150 417 255
Fragments arranged by size:
417 255 150
Bsu36I CC'TnA_GG
Cuts at: 0 605 822
Size: 605 217
BtsI GCAGTG_nn'
Cuts at: 0 407 457 822
Size: 407 50 365
Fragments arranged by size:
407 365 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 60 95 822
Size: 60 35 727
Fragments arranged by size:
727 60 35
EaeI y'GGCC_r
Cuts at: 0 247 822
Size: 247 575
EarI CTCTTCn'nnn_
Cuts at: 0 109 803 822
Size: 109 694 19
Fragments arranged by size:
694 109 19
EcoICRI GAG'CTC
Cuts at: 0 772 822
Size: 772 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 784 822
Size: 340 444 38
Fragments arranged by size:
444 340 38
EcoO109I rG'GnC_Cy
Cuts at: 0 94 426 822
Size: 94 332 396
Fragments arranged by size:
396 332 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 559 591 822
Size: 474 32 53 32 231
Fragments arranged by size:
474 231 53 32 32
MscI TGG'CCA
Cuts at: 0 249 822
Size: 249 573
MslI CAynn'nnrTG
Cuts at: 0 55 683 822
Size: 55 628 139
Fragments arranged by size:
628 139 55
MspA1I CmG'CkG
Cuts at: 0 318 518 822
Size: 318 200 304
Fragments arranged by size:
318 304 200
NsiI A_TGCA'T
Cuts at: 0 680 822
Size: 680 142
PasI CC'CwG_GG
Cuts at: 0 98 765 822
Size: 98 667 57
Fragments arranged by size:
667 98 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 822
Size: 666 156
PmlI CAC'GTG
Cuts at: 0 759 822
Size: 759 63
PpuMI rG'GwC_Cy
Cuts at: 0 94 426 822
Size: 94 332 396
Fragments arranged by size:
396 332 94
PshAI GACnn'nnGTC
Cuts at: 0 743 822
Size: 743 79
PspOMI G'GGCC_C
Cuts at: 0 101 822
Size: 101 721
PstI C_TGCA'G
Cuts at: 0 562 822
Size: 562 260
PvuII CAG'CTG
Cuts at: 0 318 518 822
Size: 318 200 304
Fragments arranged by size:
318 304 200
SacI G_AGCT'C
Cuts at: 0 774 822
Size: 774 48
SanDI GG'GwC_CC
Cuts at: 0 94 822
Size: 94 728
SapI GCTCTTCn'nnn_
Cuts at: 0 109 822
Size: 109 713
ScaI AGT'ACT
Cuts at: 0 221 822
Size: 221 601
SfcI C'TryA_G
Cuts at: 0 430 558 822
Size: 430 128 264
Fragments arranged by size:
430 264 128
SmaI CCC'GGG
Cuts at: 0 55 822
Size: 55 767
SmlI C'TyrA_G
Cuts at: 0 364 773 822
Size: 364 409 49
Fragments arranged by size:
409 364 49
SspI AAT'ATT
Cuts at: 0 702 822
Size: 702 120
StuI AGG'CCT
Cuts at: 0 332 822
Size: 332 490
StyI C'CwwG_G
Cuts at: 0 416 822
Size: 416 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 397 799 822
Size: 49 348 402 23
Fragments arranged by size:
402 348 49 23
TatI w'GTAC_w
Cuts at: 0 219 498 572 803 822
Size: 219 279 74 231 19
Fragments arranged by size:
279 231 219 74 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 822
Size: 184 32 606
Fragments arranged by size:
606 184 32
XbaI T'CTAG_A
Cuts at: 0 708 822
Size: 708 114
XmaI C'CCGG_G
Cuts at: 0 53 822
Size: 53 769
Enzymes that do cut:
AcuI AleI AlwNI ApaI AvaI BanI BanII BciVI
BfrBI BglI BglII Bme1580I BmrI BpuEI BsaAI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrGI BssSI BstYI
Bsu36I BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I MscI MslI MspA1I NsiI PasI PflMI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BclI BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PfoI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XcmI XhoI XmnI ZraI