Restriction map for
pF1KE0680
(Linear) (Six-base) MAPSORT of: KIEE0680.seq Check: 1924 from: 1 to: 1197
KIEE0680.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1197
Size: 820 377
AfeI AGC'GCT
Cuts at: 0 634 1197
Size: 634 563
AflIII A'CryG_T
Cuts at: 0 548 1197
Size: 548 649
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 959 991 1197
Size: 959 32 206
Fragments arranged by size:
959 206 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 105 1197
Size: 84 21 1092
Fragments arranged by size:
1092 84 21
AvaI C'yCGr_G
Cuts at: 0 277 896 1197
Size: 277 619 301
Fragments arranged by size:
619 301 277
BamHI G'GATC_C
Cuts at: 0 236 1197
Size: 236 961
BanII G_rGCy'C
Cuts at: 0 119 725 896 1197
Size: 119 606 171 301
Fragments arranged by size:
606 301 171 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 1197
Size: 826 371
BmgBI CAC'GTC
Cuts at: 0 824 1197
Size: 824 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 169 649 934 1197
Size: 76 93 480 285 263
Fragments arranged by size:
480 285 263 93 76
Bpu10I CC'TnA_GC
Cuts at: 0 691 1197
Size: 691 506
BsaAI yAC'GTr
Cuts at: 0 551 1197
Size: 551 646
BsaHI Gr'CG_yC
Cuts at: 0 11 1197
Size: 11 1186
BsaWI w'CCGG_w
Cuts at: 0 36 185 482 1058 1197
Size: 36 149 297 576 139
Fragments arranged by size:
576 297 149 139 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 127 295 697 1096 1197
Size: 55 72 168 402 399 101
Fragments arranged by size:
402 399 168 101 72 55
BseYI C'CCAG_C
Cuts at: 0 503 804 1197
Size: 503 301 393
Fragments arranged by size:
503 393 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1197
Size: 442 755
BsiEI CG_ry'CG
Cuts at: 0 1029 1197
Size: 1029 168
BsiHKAI G_wGCw'C
Cuts at: 0 119 377 1197
Size: 119 258 820
Fragments arranged by size:
820 258 119
Bsp1286I G_dGCh'C
Cuts at: 0 119 377 725 896 1197
Size: 119 258 348 171 301
Fragments arranged by size:
348 301 258 171 119
BspEI T'CCGG_A
Cuts at: 0 36 1197
Size: 36 1161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 1197
Size: 683 514
BsrBI CCG'CTC
Cuts at: 0 45 1197
Size: 45 1152
BsrDI GCAATG_nn'
Cuts at: 0 203 1197
Size: 203 994
BsrGI T'GTAC_A
Cuts at: 0 299 1197
Size: 299 898
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 1197
Size: 84 1113
BstYI r'GATC_y
Cuts at: 0 236 390 1061 1197
Size: 236 154 671 136
Fragments arranged by size:
671 236 154 136
Bsu36I CC'TnA_GG
Cuts at: 0 430 680 737 1197
Size: 430 250 57 460
Fragments arranged by size:
460 430 250 57
BtgI C'CryG_G
Cuts at: 0 881 1197
Size: 881 316
BtsI GCAGTG_nn'
Cuts at: 0 377 1197
Size: 377 820
DraIII CAC_nnn'GTG
Cuts at: 0 1053 1197
Size: 1053 144
EaeI y'GGCC_r
Cuts at: 0 641 1026 1197
Size: 641 385 171
Fragments arranged by size:
641 385 171
EagI C'GGCC_G
Cuts at: 0 1026 1197
Size: 1026 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 1036 1197
Size: 87 949 161
Fragments arranged by size:
949 161 87
EcoICRI GAG'CTC
Cuts at: 0 117 1197
Size: 117 1080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 169 649 820 934 1197
Size: 76 93 480 171 114 263
Fragments arranged by size:
480 263 171 114 93 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 301 679 1197
Size: 109 192 378 518
Fragments arranged by size:
518 378 192 109
EcoO109I rG'GnC_Cy
Cuts at: 0 426 672 1197
Size: 426 246 525
Fragments arranged by size:
525 426 246
HaeII r_GCGC'y
Cuts at: 0 105 636 837 1197
Size: 105 531 201 360
Fragments arranged by size:
531 360 201 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 890 922 1197
Size: 890 32 275
Fragments arranged by size:
890 275 32
MscI TGG'CCA
Cuts at: 0 643 1197
Size: 643 554
PasI CC'CwG_GG
Cuts at: 0 526 1197
Size: 526 671
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 1197
Size: 246 951
PfoI T'CCnGG_A
Cuts at: 0 611 1197
Size: 611 586
PmlI CAC'GTG
Cuts at: 0 551 1197
Size: 551 646
PpuMI rG'GwC_Cy
Cuts at: 0 672 1197
Size: 672 525
PstI C_TGCA'G
Cuts at: 0 269 1197
Size: 269 928
RsrII CG'GwC_CG
Cuts at: 0 780 1197
Size: 780 417
SacI G_AGCT'C
Cuts at: 0 119 1197
Size: 119 1078
SfcI C'TryA_G
Cuts at: 0 265 1197
Size: 265 932
StuI AGG'CCT
Cuts at: 0 496 1197
Size: 496 701
TatI w'GTAC_w
Cuts at: 0 299 875 1197
Size: 299 576 322
Fragments arranged by size:
576 322 299
Tth111I GACn'n_nGTC
Cuts at: 0 10 1197
Size: 10 1187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 1197
Size: 135 1062
XmnI GAAnn'nnTTC
Cuts at: 0 211 1197
Size: 211 986
Enzymes that do cut:
AcuI AfeI AflIII AloI AlwNI AvaI BamHI BanII
BbsI BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BstAPI BstYI Bsu36I BtgI BtsI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI PasI PflMI PfoI PmlI PpuMI PstI
RsrII SacI SfcI StuI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspHI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DrdI EarI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TstI
XbaI XhoI XmaI ZraI