(Linear) (Six-base) MAPSORT of: KIEE0667.seq  Check: 6494  from: 1  to: 729



KIEE0667.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     294     729 
   Size:         80     214     435

  Fragments arranged by size: 

                 435     214      80
 
AflIII A'CryG_T

Cuts at:      0      51     294     626     729 
   Size:         51     243     332     103

  Fragments arranged by size: 

                 332     243     103      51
 
AlwNI CAG_nnn'CTG

Cuts at:      0     101     598     729 
   Size:        101     497     131

  Fragments arranged by size: 

                 497     131     101
 
ApaLI G'TGCA_C

Cuts at:      0     622     729 
   Size:        622     107
 
ApoI r'AATT_y

Cuts at:      0     229     729 
   Size:        229     500
 
AvaI C'yCGr_G

Cuts at:      0     332     729 
   Size:        332     397
 
BanII G_rGCy'C

Cuts at:      0      37     587     729 
   Size:         37     550     142

  Fragments arranged by size: 

                 550     142      37
 
Bme1580I G_kGCm'C

Cuts at:      0     626     729 
   Size:        626     103
 
BmgBI CAC'GTC

Cuts at:      0      54     295     729 
   Size:         54     241     434

  Fragments arranged by size: 

                 434     241      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     729 
   Size:        111     618
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     729 
   Size:        392     337
 
BsaAI yAC'GTr

Cuts at:      0     627     729 
   Size:        627     102
 
BsaHI Gr'CG_yC

Cuts at:      0     699     729 
   Size:        699      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45     532     729 
   Size:         45     487     197

  Fragments arranged by size: 

                 487     197      45
 
BseYI C'CCAG_C

Cuts at:      0     446     729 
   Size:        446     283
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     729 
   Size:        415     314
 
BsiHKAI G_wGCw'C

Cuts at:      0     587     626     729 
   Size:        587      39     103

  Fragments arranged by size: 

                 587     103      39
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     587     626     729 
   Size:         37     550      39     103

  Fragments arranged by size: 

                 550     103      39      37
 
BspHI T'CATG_A

Cuts at:      0     351     729 
   Size:        351     378
 
BsrDI GCAATG_nn'

Cuts at:      0     192     729 
   Size:        192     537
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     106     729 
   Size:        106     623
 
EaeI y'GGCC_r

Cuts at:      0     554     729 
   Size:        554     175
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     647     729 
   Size:        209     438      82

  Fragments arranged by size: 

                 438     209      82
 
EcoICRI GAG'CTC

Cuts at:      0     585     729 
   Size:        585     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     294     392     729 
   Size:         80     214      98     337

  Fragments arranged by size: 

                 337     214      98      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     657     729 
   Size:        657      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     405     729 
   Size:        405     324
 
MscI TGG'CCA

Cuts at:      0     556     729 
   Size:        556     173
 
MslI CAynn'nnrTG

Cuts at:      0      25     729 
   Size:         25     704
 
PasI CC'CwG_GG

Cuts at:      0      37     729 
   Size:         37     692
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     493     729 
   Size:        493     236
 
PmlI CAC'GTG

Cuts at:      0     627     729 
   Size:        627     102
 
PpuMI rG'GwC_Cy

Cuts at:      0     657     729 
   Size:        657      72
 
PstI C_TGCA'G

Cuts at:      0     330     729 
   Size:        330     399
 
SacI G_AGCT'C

Cuts at:      0     587     729 
   Size:        587     142
 
SfcI C'TryA_G

Cuts at:      0     326     729 
   Size:        326     403
 
Tth111I GACn'n_nGTC

Cuts at:      0     600     729 
   Size:        600     129
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     494     729 
   Size:        494     235
 
XmnI GAAnn'nnTTC

Cuts at:      0     134     729 
   Size:        134     595
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI    ApaLI     ApoI     AvaI    BanII Bme1580I
    BmgBI     BmrI     BpmI    BsaAI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BsrDI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I     FalI     MscI     MslI     PasI    PflMI     PmlI
    PpuMI     PstI     SacI     SfcI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     ZraI