(Linear) (Six-base) MAPSORT of: KIEE0665.seq  Check: 1917  from: 1  to: 720



KIEE0665.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     720 
   Size:        432     288
 
AflIII A'CryG_T

Cuts at:      0     335     720 
   Size:        335     385
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     529     720 
   Size:        529     191
 
AvaI C'yCGr_G

Cuts at:      0     596     720 
   Size:        596     124
 
BanI G'GyrC_C

Cuts at:      0      28     720 
   Size:         28     692
 
BfrBI ATG'CAT

Cuts at:      0     516     720 
   Size:        516     204
 
BglII A'GATC_T

Cuts at:      0     706     720 
   Size:        706      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     171     720 
   Size:        171     549
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     311     614     720 
   Size:        311     303     106

  Fragments arranged by size: 

                 311     303     106
 
BsaBI GATnn'nnATC

Cuts at:      0     585     591     720 
   Size:        585       6     129

  Fragments arranged by size: 

                 585     129       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     720 
   Size:        259      30     431

  Fragments arranged by size: 

                 431     259      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     262     283     304     720 
   Size:        262      21      21     416

  Fragments arranged by size: 

                 416     262      21      21
 
BsiEI CG_ry'CG

Cuts at:      0     691     720 
   Size:        691      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     100     720 
   Size:        100     620
 
BsmI GAATG_Cn'

Cuts at:      0     490     720 
   Size:        490     230
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     720 
   Size:        100     620
 
BsrDI GCAATG_nn'

Cuts at:      0     134     720 
   Size:        134     586
 
BssSI C'ACGA_G

Cuts at:      0     491     720 
   Size:        491     229
 
BstYI r'GATC_y

Cuts at:      0     586     706     720 
   Size:        586     120      14

  Fragments arranged by size: 

                 586     120      14
 
BtgI C'CryG_G

Cuts at:      0     624     720 
   Size:        624      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     224     720 
   Size:        224     496
 
DraIII CAC_nnn'GTG

Cuts at:      0     327     720 
   Size:        327     393
 
EaeI y'GGCC_r

Cuts at:      0     558     720 
   Size:        558     162
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     720 
   Size:        177     543
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     680     720 
   Size:         19     661      40

  Fragments arranged by size: 

                 661      40      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     720 
   Size:        432     288
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     401     433     720 
   Size:         82      32     287      32     287

  Fragments arranged by size: 

                 287     287      82      32      32
 
HincII GTy'rAC

Cuts at:      0      48     720 
   Size:         48     672
 
MslI CAynn'nnrTG

Cuts at:      0     233     720 
   Size:        233     487
 
NsiI A_TGCA'T

Cuts at:      0     518     720 
   Size:        518     202
 
NspI r_CATG'y

Cuts at:      0      80     720 
   Size:         80     640
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     327     720 
   Size:        327     393
 
PshAI GACnn'nnGTC

Cuts at:      0     626     720 
   Size:        626      94
 
SexAI A'CCwGG_T

Cuts at:      0     323     720 
   Size:        323     397
 
SmaI CCC'GGG

Cuts at:      0     598     720 
   Size:        598     122
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     602     720 
   Size:        602     118
 
Tth111I GACn'n_nGTC

Cuts at:      0      43     720 
   Size:         43     677
 
XbaI T'CTAG_A

Cuts at:      0     616     720 
   Size:        616     104
 
XmaI C'CCGG_G

Cuts at:      0     596     720 
   Size:        596     124
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     AvaI     BanI    BfrBI    BglII     BmrI
     BsaI    BsaBI    BsaXI    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BsrDI    BssSI    BstYI     BtgI    BtgZI   DraIII     EaeI     EarI
     EciI  Eco57MI    Hin4I   HincII     MslI     NsiI     NspI    PflMI
    PshAI    SexAI     SmaI    TaqII  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BseYI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI     XcmI     XhoI     XmnI     ZraI