Restriction map for
pF1KE0661
(Linear) (Six-base) MAPSORT of: KIEE0661.seq Check: 1879 from: 1 to: 708
KIEE0661.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AflIII A'CryG_T
Cuts at: 0 405 708
Size: 405 303
AlwNI CAG_nnn'CTG
Cuts at: 0 424 487 490 550 646 649 687 708
Size: 424 63 3 60 96 3 38 21
Fragments arranged by size:
424 96 63 60 38 21 3 3
AvaI C'yCGr_G
Cuts at: 0 152 708
Size: 152 556
BamHI G'GATC_C
Cuts at: 0 210 708
Size: 210 498
BanI G'GyrC_C
Cuts at: 0 612 708
Size: 612 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 708
Size: 90 618
BbvCI CC'TCA_GC
Cuts at: 0 680 708
Size: 680 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 72 106 708
Size: 72 34 602
Fragments arranged by size:
602 72 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 341 708
Size: 341 367
BglI GCCn_nnn'nGGC
Cuts at: 0 199 708
Size: 199 509
BglII A'GATC_T
Cuts at: 0 35 708
Size: 35 673
BmgBI CAC'GTC
Cuts at: 0 408 708
Size: 408 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 527 708
Size: 479 48 181
Fragments arranged by size:
479 181 48
Bpu10I CC'TnA_GC
Cuts at: 0 680 708
Size: 680 28
BsaBI GATnn'nnATC
Cuts at: 0 215 708
Size: 215 493
BsaHI Gr'CG_yC
Cuts at: 0 71 708
Size: 71 637
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 708
Size: 127 581
BseYI C'CCAG_C
Cuts at: 0 568 708
Size: 568 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 708
Size: 403 305
BsiEI CG_ry'CG
Cuts at: 0 135 708
Size: 135 573
BsmI GAATG_Cn'
Cuts at: 0 707 708
Size: 707 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 708
Size: 360 348
BsrFI r'CCGG_y
Cuts at: 0 134 708
Size: 134 574
BstAPI GCAn_nnn'nTGC
Cuts at: 0 490 646 649 708
Size: 490 156 3 59
Fragments arranged by size:
490 156 59 3
BstYI r'GATC_y
Cuts at: 0 35 210 708
Size: 35 175 498
Fragments arranged by size:
498 175 35
BtgI C'CryG_G
Cuts at: 0 173 708
Size: 173 535
DrdI GACnn_nn'nnGTC
Cuts at: 0 147 708
Size: 147 561
EaeI y'GGCC_r
Cuts at: 0 666 708
Size: 666 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 527 708
Size: 479 48 181
Fragments arranged by size:
479 181 48
EcoO109I rG'GnC_Cy
Cuts at: 0 200 708
Size: 200 508
FspI TGC'GCA
Cuts at: 0 82 708
Size: 82 626
MscI TGG'CCA
Cuts at: 0 668 708
Size: 668 40
MspA1I CmG'CkG
Cuts at: 0 424 487 508 547 610 625 637 646
Size: 424 63 21 39 63 15 12 9
Cuts at: 646 655 687 708
Size: 9 32 21
Fragments arranged by size:
424 63 63 39 32 21 21 15
12 9 9
NcoI C'CATG_G
Cuts at: 0 173 708
Size: 173 535
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 241 273 708
Size: 241 32 435
Fragments arranged by size:
435 241 32
PstI C_TGCA'G
Cuts at: 0 555 642 660 692 708
Size: 555 87 18 32 16
Fragments arranged by size:
555 87 32 18 16
PvuII CAG'CTG
Cuts at: 0 424 487 547 625 646 687 708
Size: 424 63 60 78 21 41 21
Fragments arranged by size:
424 78 63 60 41 21 21
ScaI AGT'ACT
Cuts at: 0 88 708
Size: 88 620
SexAI A'CCwGG_T
Cuts at: 0 288 708
Size: 288 420
SfcI C'TryA_G
Cuts at: 0 551 638 656 688 708
Size: 551 87 18 32 20
Fragments arranged by size:
551 87 32 20 18
SmaI CCC'GGG
Cuts at: 0 154 708
Size: 154 554
StyI C'CwwG_G
Cuts at: 0 173 274 708
Size: 173 101 434
Fragments arranged by size:
434 173 101
TatI w'GTAC_w
Cuts at: 0 86 708
Size: 86 622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 708
Size: 249 459
XmaI C'CCGG_G
Cuts at: 0 152 708
Size: 152 556
Enzymes that do cut:
AflIII AlwNI AvaI BamHI BanI BbsI BbvCI BcgI
BciVI BglI BglII BmgBI BpmI Bpu10I BsaBI BsaHI
BseRI BseYI BsgI BsiEI BsmI BspMI BsrFI BstAPI
BstYI BtgI DrdI EaeI Eco57MI EcoO109I FspI MscI
MspA1I NcoI PsrI PstI PvuII ScaI SexAI SfcI
SmaI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanII BbeI BclI
BfrBI BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI
BsaAI BsaWI BsaXI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI