Restriction map for
pF1KE0657
(Linear) (Six-base) MAPSORT of: KIEE0657.seq Check: 3501 from: 1 to: 699
KIEE0657.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 154 699
Size: 25 129 545
Fragments arranged by size:
545 129 25
AflIII A'CryG_T
Cuts at: 0 692 699
Size: 692 7
ApaLI G'TGCA_C
Cuts at: 0 139 699
Size: 139 560
ApoI r'AATT_y
Cuts at: 0 247 384 699
Size: 247 137 315
Fragments arranged by size:
315 247 137
AvaI C'yCGr_G
Cuts at: 0 317 699
Size: 317 382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 269 699
Size: 235 34 430
Fragments arranged by size:
430 235 34
BfrBI ATG'CAT
Cuts at: 0 216 699
Size: 216 483
Bme1580I G_kGCm'C
Cuts at: 0 143 699
Size: 143 556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 437 699
Size: 146 291 262
Fragments arranged by size:
291 262 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 699
Size: 508 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 699
Size: 449 250
BsaHI Gr'CG_yC
Cuts at: 0 621 699
Size: 621 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 9 39 470 500 699
Size: 9 30 431 30 199
Fragments arranged by size:
431 199 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 467 699
Size: 467 232
BsiHKAI G_wGCw'C
Cuts at: 0 143 699
Size: 143 556
Bsp1286I G_dGCh'C
Cuts at: 0 143 699
Size: 143 556
BsrBI CCG'CTC
Cuts at: 0 66 699
Size: 66 633
BsrGI T'GTAC_A
Cuts at: 0 184 699
Size: 184 515
BstAPI GCAn_nnn'nTGC
Cuts at: 0 280 289 639 699
Size: 280 9 350 60
Fragments arranged by size:
350 280 60 9
BtgI C'CryG_G
Cuts at: 0 29 699
Size: 29 670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 146 154 437 699
Size: 25 121 8 283 262
Fragments arranged by size:
283 262 121 25 8
EcoRI G'AATT_C
Cuts at: 0 247 699
Size: 247 452
MfeI C'AATT_G
Cuts at: 0 95 637 699
Size: 95 542 62
Fragments arranged by size:
542 95 62
MspA1I CmG'CkG
Cuts at: 0 462 699
Size: 462 237
NsiI A_TGCA'T
Cuts at: 0 218 699
Size: 218 481
NspI r_CATG'y
Cuts at: 0 696 699
Size: 696 3
PciI A'CATG_T
Cuts at: 0 692 699
Size: 692 7
PfoI T'CCnGG_A
Cuts at: 0 414 699
Size: 414 285
PshAI GACnn'nnGTC
Cuts at: 0 55 699
Size: 55 644
PspXI vC'TCGA_Gb
Cuts at: 0 317 699
Size: 317 382
PvuII CAG'CTG
Cuts at: 0 462 699
Size: 462 237
SfcI C'TryA_G
Cuts at: 0 115 699
Size: 115 584
SmlI C'TyrA_G
Cuts at: 0 317 523 699
Size: 317 206 176
Fragments arranged by size:
317 206 176
StuI AGG'CCT
Cuts at: 0 350 443 699
Size: 350 93 256
Fragments arranged by size:
350 256 93
StyI C'CwwG_G
Cuts at: 0 351 699
Size: 351 348
TatI w'GTAC_w
Cuts at: 0 184 699
Size: 184 515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 9 41 470 502 699
Size: 9 32 429 32 197
Fragments arranged by size:
429 197 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 541 699
Size: 541 158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 699
Size: 36 663
XhoI C'TCGA_G
Cuts at: 0 317 699
Size: 317 382
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AvaI BcgI BfrBI Bme1580I
BpmI BpuEI BsaI BsaHI BsaXI BseRI BsiHKAI Bsp1286I
BsrBI BsrGI BstAPI BtgI Eco57MI EcoRI MfeI MspA1I
NsiI NspI PciI PfoI PshAI PspXI PvuII SfcI
SmlI StuI StyI TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XmaI XmnI ZraI