Restriction map for
pF1KE0652
(Linear) (Six-base) MAPSORT of: KIEE0652.seq Check: 5122 from: 1 to: 1155
KIEE0652.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 140 196 590 1155
Size: 68 72 56 394 565
Fragments arranged by size:
565 394 72 68 56
AflIII A'CryG_T
Cuts at: 0 803 805 1155
Size: 803 2 350
Fragments arranged by size:
803 350 2
AhdI GACnn_n'nnGTC
Cuts at: 0 612 1155
Size: 612 543
AleI CACnn'nnGTG
Cuts at: 0 1126 1155
Size: 1126 29
ApaLI G'TGCA_C
Cuts at: 0 1138 1155
Size: 1138 17
AvaI C'yCGr_G
Cuts at: 0 5 1155
Size: 5 1150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 792 825 1155
Size: 792 33 330
Fragments arranged by size:
792 330 33
BanI G'GyrC_C
Cuts at: 0 824 1044 1155
Size: 824 220 111
Fragments arranged by size:
824 220 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 260 1155
Size: 56 204 895
Fragments arranged by size:
895 204 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 802 836 986 1020 1155
Size: 802 34 150 34 135
Fragments arranged by size:
802 150 135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 169 1155
Size: 169 986
BclI T'GATC_A
Cuts at: 0 485 1155
Size: 485 670
BglII A'GATC_T
Cuts at: 0 62 1155
Size: 62 1093
Bme1580I G_kGCm'C
Cuts at: 0 1142 1155
Size: 1142 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 741 1155
Size: 741 414
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 738 1155
Size: 42 696 417
Fragments arranged by size:
696 417 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1155
Size: 536 619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 685 1155
Size: 418 267 470
Fragments arranged by size:
470 418 267
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 422 1092 1104 1155
Size: 73 349 670 12 51
Fragments arranged by size:
670 349 73 51 12
BsaAI yAC'GTr
Cuts at: 0 806 1155
Size: 806 349
BsaHI Gr'CG_yC
Cuts at: 0 389 1155
Size: 389 766
BsaWI w'CCGG_w
Cuts at: 0 304 323 890 1155
Size: 304 19 567 265
Fragments arranged by size:
567 304 265 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 1155
Size: 325 30 800
Fragments arranged by size:
800 325 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 484 1155
Size: 18 466 671
Fragments arranged by size:
671 466 18
BsiEI CG_ry'CG
Cuts at: 0 941 1155
Size: 941 214
BsiHKAI G_wGCw'C
Cuts at: 0 1142 1155
Size: 1142 13
Bsp1286I G_dGCh'C
Cuts at: 0 1142 1155
Size: 1142 13
BspEI T'CCGG_A
Cuts at: 0 890 1155
Size: 890 265
BsrBI CCG'CTC
Cuts at: 0 1107 1155
Size: 1107 48
BssSI C'ACGA_G
Cuts at: 0 868 1155
Size: 868 287
BstAPI GCAn_nnn'nTGC
Cuts at: 0 931 1155
Size: 931 224
BstYI r'GATC_y
Cuts at: 0 62 695 887 1155
Size: 62 633 192 268
Fragments arranged by size:
633 268 192 62
BtgI C'CryG_G
Cuts at: 0 829 935 1155
Size: 829 106 220
Fragments arranged by size:
829 220 106
BtsI GCAGTG_nn'
Cuts at: 0 536 1142 1155
Size: 536 606 13
Fragments arranged by size:
606 536 13
EaeI y'GGCC_r
Cuts at: 0 938 1022 1155
Size: 938 84 133
Fragments arranged by size:
938 133 84
EagI C'GGCC_G
Cuts at: 0 938 1155
Size: 938 217
EarI CTCTTCn'nnn_
Cuts at: 0 116 1155
Size: 116 1039
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 364 721 1155
Size: 364 357 434
Fragments arranged by size:
434 364 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 140 196 536 590 1155
Size: 68 72 56 340 54 565
Fragments arranged by size:
565 340 72 68 56 54
EcoO109I rG'GnC_Cy
Cuts at: 0 95 786 1155
Size: 95 691 369
Fragments arranged by size:
691 369 95
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 1155
Size: 81 1074
HaeII r_GCGC'y
Cuts at: 0 175 884 1155
Size: 175 709 271
Fragments arranged by size:
709 271 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 517 549 598 630 727 759
Size: 457 32 28 32 49 32 97 32
Cuts at: 759 1155
Size: 396
Fragments arranged by size:
457 396 97 49 32 32 32 32
28
HincII GTy'rAC
Cuts at: 0 606 1155
Size: 606 549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1077 1155
Size: 770 307 78
Fragments arranged by size:
770 307 78
MscI TGG'CCA
Cuts at: 0 1024 1155
Size: 1024 131
MslI CAynn'nnrTG
Cuts at: 0 622 1126 1155
Size: 622 504 29
Fragments arranged by size:
622 504 29
MspA1I CmG'CkG
Cuts at: 0 297 1155
Size: 297 858
NcoI C'CATG_G
Cuts at: 0 829 1155
Size: 829 326
NdeI CA'TA_TG
Cuts at: 0 953 1155
Size: 953 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 411 1155
Size: 384 27 744
Fragments arranged by size:
744 384 27
PfoI T'CCnGG_A
Cuts at: 0 31 1155
Size: 31 1124
PmlI CAC'GTG
Cuts at: 0 806 1155
Size: 806 349
PpuMI rG'GwC_Cy
Cuts at: 0 786 1155
Size: 786 369
PvuII CAG'CTG
Cuts at: 0 297 1155
Size: 297 858
SfcI C'TryA_G
Cuts at: 0 456 1059 1155
Size: 456 603 96
Fragments arranged by size:
603 456 96
SmaI CCC'GGG
Cuts at: 0 7 1155
Size: 7 1148
SmlI C'TyrA_G
Cuts at: 0 433 700 1155
Size: 433 267 455
Fragments arranged by size:
455 433 267
SspI AAT'ATT
Cuts at: 0 240 1155
Size: 240 915
StyI C'CwwG_G
Cuts at: 0 829 1155
Size: 829 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 842 1155
Size: 842 313
TatI w'GTAC_w
Cuts at: 0 281 395 452 1155
Size: 281 114 57 703
Fragments arranged by size:
703 281 114 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 825 857 1155
Size: 825 32 298
Fragments arranged by size:
825 298 32
XmaI C'CCGG_G
Cuts at: 0 5 1155
Size: 5 1150
XmnI GAAnn'nnTTC
Cuts at: 0 64 1155
Size: 64 1091
Enzymes that do cut:
AcuI AflIII AhdI AleI ApaLI AvaI BaeI BanI
BbsI BcgI BciVI BclI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsiEI BsiHKAI Bsp1286I BspEI BsrBI BssSI BstAPI BstYI
BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NcoI NdeI PflMI PfoI PmlI PpuMI PvuII SfcI
SmaI SmlI SspI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbvCI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BsaBI BseYI BsgI BsiWI BsmI
BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XcmI XhoI ZraI