Restriction map for
pF1KE0648
(Linear) (Six-base) MAPSORT of: KIEE0648.seq Check: 1794 from: 1 to: 1140
KIEE0648.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 1022 1140
Size: 1022 118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 232 600 1140
Size: 205 27 368 540
Fragments arranged by size:
540 368 205 27
AhdI GACnn_n'nnGTC
Cuts at: 0 939 1140
Size: 939 201
AlwNI CAG_nnn'CTG
Cuts at: 0 60 606 1140
Size: 60 546 534
Fragments arranged by size:
546 534 60
ApaI G_GGCC'C
Cuts at: 0 821 1134 1140
Size: 821 313 6
Fragments arranged by size:
821 313 6
AvaI C'yCGr_G
Cuts at: 0 694 719 1140
Size: 694 25 421
Fragments arranged by size:
694 421 25
BanI G'GyrC_C
Cuts at: 0 313 553 875 1083 1140
Size: 313 240 322 208 57
Fragments arranged by size:
322 313 240 208 57
BanII G_rGCy'C
Cuts at: 0 196 580 641 821 1134 1140
Size: 196 384 61 180 313 6
Fragments arranged by size:
384 313 196 180 61 6
BbeI G_GCGC'C
Cuts at: 0 317 557 1087 1140
Size: 317 240 530 53
Fragments arranged by size:
530 317 240 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1140
Size: 253 887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 533 1140
Size: 499 34 607
Fragments arranged by size:
607 499 34
BglI GCCn_nnn'nGGC
Cuts at: 0 367 731 1140
Size: 367 364 409
Fragments arranged by size:
409 367 364
BlpI GC'TnA_GC
Cuts at: 0 562 1140
Size: 562 578
Bme1580I G_kGCm'C
Cuts at: 0 821 1134 1140
Size: 821 313 6
Fragments arranged by size:
821 313 6
BmgBI CAC'GTC
Cuts at: 0 110 1140
Size: 110 1030
Bpu10I CC'TnA_GC
Cuts at: 0 538 1140
Size: 538 602
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 1140
Size: 652 488
BsaAI yAC'GTr
Cuts at: 0 165 753 1140
Size: 165 588 387
Fragments arranged by size:
588 387 165
BsaHI Gr'CG_yC
Cuts at: 0 287 314 554 1084 1140
Size: 287 27 240 530 56
Fragments arranged by size:
530 287 240 56 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 1140
Size: 370 30 740
Fragments arranged by size:
740 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 646 649 1140
Size: 514 132 3 491
Fragments arranged by size:
514 491 132 3
BseYI C'CCAG_C
Cuts at: 0 44 277 949 1140
Size: 44 233 672 191
Fragments arranged by size:
672 233 191 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 766 1024 1140
Size: 450 316 258 116
Fragments arranged by size:
450 316 258 116
BsiEI CG_ry'CG
Cuts at: 0 321 1140
Size: 321 819
BsiHKAI G_wGCw'C
Cuts at: 0 641 1106 1140
Size: 641 465 34
Fragments arranged by size:
641 465 34
Bsp1286I G_dGCh'C
Cuts at: 0 196 580 641 821 1106 1134 1140
Size: 196 384 61 180 285 28 6
Fragments arranged by size:
384 285 196 180 61 28 6
BsrBI CCG'CTC
Cuts at: 0 81 171 375 903 1140
Size: 81 90 204 528 237
Fragments arranged by size:
528 237 204 90 81
BsrDI GCAATG_nn'
Cuts at: 0 573 1140
Size: 573 567
BssHII G'CGCG_C
Cuts at: 0 254 1140
Size: 254 886
BssSI C'ACGA_G
Cuts at: 0 199 1009 1140
Size: 199 810 131
Fragments arranged by size:
810 199 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 1140
Size: 165 975
BtgI C'CryG_G
Cuts at: 0 662 1052 1140
Size: 662 390 88
Fragments arranged by size:
662 390 88
EaeI y'GGCC_r
Cuts at: 0 131 368 570 1140
Size: 131 237 202 570
Fragments arranged by size:
570 237 202 131
EcoICRI GAG'CTC
Cuts at: 0 639 1140
Size: 639 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 232 600 1140
Size: 205 27 368 540
Fragments arranged by size:
540 368 205 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 397 547 1140
Size: 187 210 150 593
Fragments arranged by size:
593 210 187 150
EcoO109I rG'GnC_Cy
Cuts at: 0 818 1130 1131 1140
Size: 818 312 1 9
Fragments arranged by size:
818 312 9 1
FspI TGC'GCA
Cuts at: 0 547 791 1140
Size: 547 244 349
Fragments arranged by size:
547 349 244
HaeII r_GCGC'y
Cuts at: 0 317 557 837 1044 1087 1140
Size: 317 240 280 207 43 53
Fragments arranged by size:
317 280 240 207 53 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 925 957 1140
Size: 373 32 520 32 183
Fragments arranged by size:
520 373 183 32 32
HindIII A'AGCT_T
Cuts at: 0 531 1140
Size: 531 609
KasI G'GCGC_C
Cuts at: 0 313 553 1083 1140
Size: 313 240 530 57
Fragments arranged by size:
530 313 240 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 509 1140
Size: 182 327 631
Fragments arranged by size:
631 327 182
MscI TGG'CCA
Cuts at: 0 572 1140
Size: 572 568
MslI CAynn'nnrTG
Cuts at: 0 418 667 1140
Size: 418 249 473
Fragments arranged by size:
473 418 249
MspA1I CmG'CkG
Cuts at: 0 311 830 1038 1140
Size: 311 519 208 102
Fragments arranged by size:
519 311 208 102
NarI GG'CG_CC
Cuts at: 0 314 554 1084 1140
Size: 314 240 530 56
Fragments arranged by size:
530 314 240 56
NcoI C'CATG_G
Cuts at: 0 662 1052 1140
Size: 662 390 88
Fragments arranged by size:
662 390 88
NspI r_CATG'y
Cuts at: 0 595 1140
Size: 595 545
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 110 1053 1140
Size: 60 50 943 87
Fragments arranged by size:
943 87 60 50
PspOMI G'GGCC_C
Cuts at: 0 817 1130 1140
Size: 817 313 10
Fragments arranged by size:
817 313 10
PstI C_TGCA'G
Cuts at: 0 1139 1140
Size: 1139 1
SacI G_AGCT'C
Cuts at: 0 641 1140
Size: 641 499
SfcI C'TryA_G
Cuts at: 0 1135 1140
Size: 1135 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 731 1140
Size: 731 409
SfoI GGC'GCC
Cuts at: 0 315 555 1085 1140
Size: 315 240 530 55
Fragments arranged by size:
530 315 240 55
SmaI CCC'GGG
Cuts at: 0 721 1140
Size: 721 419
StuI AGG'CCT
Cuts at: 0 227 1140
Size: 227 913
StyI C'CwwG_G
Cuts at: 0 662 1052 1140
Size: 662 390 88
Fragments arranged by size:
662 390 88
Tth111I GACn'n_nGTC
Cuts at: 0 1018 1140
Size: 1018 122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 750 1059 1140
Size: 492 258 309 81
Fragments arranged by size:
492 309 258 81
XmaI C'CCGG_G
Cuts at: 0 719 1140
Size: 719 421
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BsrDI BssHII BssSI BstXI BtgI
EaeI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PflMI PspOMI PstI SacI SfcI SfiI SfoI
SmaI StuI StyI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmrI BmtI BplI BpmI BpuEI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoRI
EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI TstI XbaI XhoI XmnI ZraI