Restriction map for
pF1KE0637
(Linear) (Six-base) MAPSORT of: KIEE0637.seq Check: 370 from: 1 to: 627
KIEE0637.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
Acc65I G'GTAC_C
Cuts at: 0 575 627
Size: 575 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 627
Size: 31 596
AlwNI CAG_nnn'CTG
Cuts at: 0 300 627
Size: 300 327
ApaI G_GGCC'C
Cuts at: 0 468 627
Size: 468 159
ApaLI G'TGCA_C
Cuts at: 0 376 627
Size: 376 251
AvrII C'CTAG_G
Cuts at: 0 387 627
Size: 387 240
BanI G'GyrC_C
Cuts at: 0 43 70 575 627
Size: 43 27 505 52
Fragments arranged by size:
505 52 43 27
BanII G_rGCy'C
Cuts at: 0 64 468 627
Size: 64 404 159
Fragments arranged by size:
404 159 64
BbeI G_GCGC'C
Cuts at: 0 74 627
Size: 74 553
BclI T'GATC_A
Cuts at: 0 182 627
Size: 182 445
Bme1580I G_kGCm'C
Cuts at: 0 380 468 627
Size: 380 88 159
Fragments arranged by size:
380 159 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 627
Size: 97 530
Bpu10I CC'TnA_GC
Cuts at: 0 563 627
Size: 563 64
BsaHI Gr'CG_yC
Cuts at: 0 71 627
Size: 71 556
BsaWI w'CCGG_w
Cuts at: 0 89 627
Size: 89 538
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 627
Size: 94 3 530
Fragments arranged by size:
530 94 3
BseYI C'CCAG_C
Cuts at: 0 367 498 596 627
Size: 367 131 98 31
Fragments arranged by size:
367 131 98 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 627
Size: 254 373
BsiEI CG_ry'CG
Cuts at: 0 157 627
Size: 157 470
BsiHKAI G_wGCw'C
Cuts at: 0 29 380 396 627
Size: 29 351 16 231
Fragments arranged by size:
351 231 29 16
BsmI GAATG_Cn'
Cuts at: 0 261 627
Size: 261 366
Bsp1286I G_dGCh'C
Cuts at: 0 29 64 380 396 468 627
Size: 29 35 316 16 72 159
Fragments arranged by size:
316 159 72 35 29 16
BspEI T'CCGG_A
Cuts at: 0 89 627
Size: 89 538
BsrFI r'CCGG_y
Cuts at: 0 40 46 627
Size: 40 6 581
Fragments arranged by size:
581 40 6
BsrGI T'GTAC_A
Cuts at: 0 479 627
Size: 479 148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 461 627
Size: 461 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 627
Size: 387 240
BtsI GCAGTG_nn'
Cuts at: 0 191 536 627
Size: 191 345 91
Fragments arranged by size:
345 191 91
EaeI y'GGCC_r
Cuts at: 0 154 248 335 627
Size: 154 94 87 292
Fragments arranged by size:
292 154 94 87
EagI C'GGCC_G
Cuts at: 0 154 627
Size: 154 473
EarI CTCTTCn'nnn_
Cuts at: 0 154 627
Size: 154 473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 97 627
Size: 31 66 530
Fragments arranged by size:
530 66 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 627
Size: 79 548
EcoO109I rG'GnC_Cy
Cuts at: 0 176 411 465 627
Size: 176 235 54 162
Fragments arranged by size:
235 176 162 54
HaeII r_GCGC'y
Cuts at: 0 74 627
Size: 74 553
KasI G'GCGC_C
Cuts at: 0 70 627
Size: 70 557
KpnI G_GTAC'C
Cuts at: 0 579 627
Size: 579 48
MscI TGG'CCA
Cuts at: 0 337 627
Size: 337 290
MslI CAynn'nnrTG
Cuts at: 0 331 627
Size: 331 296
MspA1I CmG'CkG
Cuts at: 0 107 544 627
Size: 107 437 83
Fragments arranged by size:
437 107 83
NarI GG'CG_CC
Cuts at: 0 71 627
Size: 71 556
PpuMI rG'GwC_Cy
Cuts at: 0 176 411 627
Size: 176 235 216
Fragments arranged by size:
235 216 176
PspOMI G'GGCC_C
Cuts at: 0 464 627
Size: 464 163
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 604 627
Size: 604 23
SanDI GG'GwC_CC
Cuts at: 0 176 411 627
Size: 176 235 216
Fragments arranged by size:
235 216 176
SapI GCTCTTCn'nnn_
Cuts at: 0 154 627
Size: 154 473
SfoI GGC'GCC
Cuts at: 0 72 627
Size: 72 555
StuI AGG'CCT
Cuts at: 0 460 627
Size: 460 167
StyI C'CwwG_G
Cuts at: 0 387 627
Size: 387 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 627
Size: 610 17
TatI w'GTAC_w
Cuts at: 0 479 627
Size: 479 148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 627
Size: 330 297
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApaLI AvrII BanI BanII
BbeI BclI Bme1580I BpmI Bpu10I BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrFI
BsrGI BstXI BtgZI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII KasI KpnI MscI MslI MspA1I
NarI PpuMI PspOMI PsrI SanDI SapI SfoI StuI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaXI BsiWI BsmBI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI