Restriction map for
pF1KE0633
(Linear) (Six-base) MAPSORT of: KIEE0633.seq Check: 3462 from: 1 to: 606
KIEE0633.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AgeI A'CCGG_T
Cuts at: 0 92 606
Size: 92 514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 354 606
Size: 354 252
ApaLI G'TGCA_C
Cuts at: 0 301 606
Size: 301 305
AvaI C'yCGr_G
Cuts at: 0 316 410 436 591 606
Size: 316 94 26 155 15
Fragments arranged by size:
316 155 94 26 15
BanII G_rGCy'C
Cuts at: 0 598 606
Size: 598 8
BclI T'GATC_A
Cuts at: 0 464 606
Size: 464 142
BfrBI ATG'CAT
Cuts at: 0 341 606
Size: 341 265
Bme1580I G_kGCm'C
Cuts at: 0 305 606
Size: 305 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 606
Size: 351 255
BsaWI w'CCGG_w
Cuts at: 0 92 233 606
Size: 92 141 373
Fragments arranged by size:
373 141 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 606
Size: 82 30 494
Fragments arranged by size:
494 82 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 537 606
Size: 256 281 69
Fragments arranged by size:
281 256 69
BsiHKAI G_wGCw'C
Cuts at: 0 100 305 606
Size: 100 205 301
Fragments arranged by size:
301 205 100
Bsp1286I G_dGCh'C
Cuts at: 0 100 305 598 606
Size: 100 205 293 8
Fragments arranged by size:
293 205 100 8
BspEI T'CCGG_A
Cuts at: 0 233 606
Size: 233 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 391 606
Size: 391 215
BsrBI CCG'CTC
Cuts at: 0 255 606
Size: 255 351
BsrFI r'CCGG_y
Cuts at: 0 80 92 606
Size: 80 12 514
Fragments arranged by size:
514 80 12
BssSI C'ACGA_G
Cuts at: 0 208 606
Size: 208 398
BstAPI GCAn_nnn'nTGC
Cuts at: 0 300 606
Size: 300 306
BstEII G'GTnAC_C
Cuts at: 0 403 606
Size: 403 203
BtgI C'CryG_G
Cuts at: 0 260 606
Size: 260 346
DraIII CAC_nnn'GTG
Cuts at: 0 210 606
Size: 210 396
EaeI y'GGCC_r
Cuts at: 0 425 476 606
Size: 425 51 130
Fragments arranged by size:
425 130 51
EarI CTCTTCn'nnn_
Cuts at: 0 167 606
Size: 167 439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 606
Size: 351 255
EcoO109I rG'GnC_Cy
Cuts at: 0 390 489 540 606
Size: 390 99 51 66
Fragments arranged by size:
390 99 66 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 434 466 532 564 606
Size: 82 32 320 32 66 32 42
Fragments arranged by size:
320 82 66 42 32 32 32
HincII GTy'rAC
Cuts at: 0 48 606
Size: 48 558
HpaI GTT'AAC
Cuts at: 0 48 606
Size: 48 558
MscI TGG'CCA
Cuts at: 0 427 606
Size: 427 179
MslI CAynn'nnrTG
Cuts at: 0 308 472 606
Size: 308 164 134
Fragments arranged by size:
308 164 134
MspA1I CmG'CkG
Cuts at: 0 281 606
Size: 281 325
NaeI GCC'GGC
Cuts at: 0 82 606
Size: 82 524
NgoMIV G'CCGG_C
Cuts at: 0 80 606
Size: 80 526
NsiI A_TGCA'T
Cuts at: 0 343 606
Size: 343 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 210 606
Size: 210 396
PpuMI rG'GwC_Cy
Cuts at: 0 390 540 606
Size: 390 150 66
Fragments arranged by size:
390 150 66
PstI C_TGCA'G
Cuts at: 0 152 606
Size: 152 454
SanDI GG'GwC_CC
Cuts at: 0 390 606
Size: 390 216
SfcI C'TryA_G
Cuts at: 0 148 606
Size: 148 458
SmaI CCC'GGG
Cuts at: 0 593 606
Size: 593 13
SspI AAT'ATT
Cuts at: 0 10 606
Size: 10 596
StyI C'CwwG_G
Cuts at: 0 195 606
Size: 195 411
XmaI C'CCGG_G
Cuts at: 0 591 606
Size: 591 15
Enzymes that do cut:
AgeI AlfI ApaLI AvaI BanII BclI BfrBI Bme1580I
BpmI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstAPI BstEII BtgI DraIII EaeI
EarI Eco57MI EcoO109I Hin4I HincII HpaI MscI MslI
MspA1I NaeI NgoMIV NsiI PflMI PpuMI PstI SanDI
SfcI SmaI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI