Restriction map for
pF1KE0631
(Linear) (Six-base) MAPSORT of: KIEE0631.seq Check: 4137 from: 1 to: 600
KIEE0631.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 0 557 600
Size: 557 43
AflIII A'CryG_T
Cuts at: 0 134 600
Size: 134 466
AgeI A'CCGG_T
Cuts at: 0 156 600
Size: 156 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 437 600
Size: 232 205 163
Fragments arranged by size:
232 205 163
ApaI G_GGCC'C
Cuts at: 0 80 177 383 600
Size: 80 97 206 217
Fragments arranged by size:
217 206 97 80
ApaLI G'TGCA_C
Cuts at: 0 400 600
Size: 400 200
ApoI r'AATT_y
Cuts at: 0 473 600
Size: 473 127
BanII G_rGCy'C
Cuts at: 0 80 177 383 600
Size: 80 97 206 217
Fragments arranged by size:
217 206 97 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 578 600
Size: 267 311 22
Fragments arranged by size:
311 267 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 282 316 459 493 600
Size: 282 34 143 34 107
Fragments arranged by size:
282 143 107 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 172 600
Size: 172 428
Bme1580I G_kGCm'C
Cuts at: 0 80 177 383 404 600
Size: 80 97 206 21 196
Fragments arranged by size:
206 196 97 80 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 600
Size: 199 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 600
Size: 175 425
BsaAI yAC'GTr
Cuts at: 0 15 600
Size: 15 585
BsaHI Gr'CG_yC
Cuts at: 0 164 554 600
Size: 164 390 46
Fragments arranged by size:
390 164 46
BsaWI w'CCGG_w
Cuts at: 0 156 600
Size: 156 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 600
Size: 334 30 236
Fragments arranged by size:
334 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 600
Size: 598 2
BseYI C'CCAG_C
Cuts at: 0 73 600
Size: 73 527
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 600
Size: 10 590
BsiHKAI G_wGCw'C
Cuts at: 0 404 600
Size: 404 196
Bsp1286I G_dGCh'C
Cuts at: 0 80 177 383 404 600
Size: 80 97 206 21 196
Fragments arranged by size:
206 196 97 80 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 600
Size: 32 568
BsrBI CCG'CTC
Cuts at: 0 122 260 429 600
Size: 122 138 169 171
Fragments arranged by size:
171 169 138 122
BsrDI GCAATG_nn'
Cuts at: 0 53 600
Size: 53 547
BsrFI r'CCGG_y
Cuts at: 0 156 600
Size: 156 444
BsrGI T'GTAC_A
Cuts at: 0 317 600
Size: 317 283
BstEII G'GTnAC_C
Cuts at: 0 36 600
Size: 36 564
Bsu36I CC'TnA_GG
Cuts at: 0 32 600
Size: 32 568
ClaI AT'CG_AT
Cuts at: 0 437 600
Size: 437 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 600
Size: 199 401
EcoNI CCTnn'n_nnAGG
Cuts at: 0 531 600
Size: 531 69
EcoO109I rG'GnC_Cy
Cuts at: 0 174 240 379 600
Size: 174 66 139 221
Fragments arranged by size:
221 174 139 66
FspI TGC'GCA
Cuts at: 0 220 600
Size: 220 380
FspAI rTGC'GCAy
Cuts at: 0 220 600
Size: 220 380
HaeII r_GCGC'y
Cuts at: 0 316 600
Size: 316 284
HincII GTy'rAC
Cuts at: 0 144 600
Size: 144 456
MluI A'CGCG_T
Cuts at: 0 134 600
Size: 134 466
MslI CAynn'nnrTG
Cuts at: 0 439 600
Size: 439 161
MspA1I CmG'CkG
Cuts at: 0 73 600
Size: 73 527
NspI r_CATG'y
Cuts at: 0 456 600
Size: 456 144
PpuMI rG'GwC_Cy
Cuts at: 0 240 600
Size: 240 360
PspOMI G'GGCC_C
Cuts at: 0 76 173 379 600
Size: 76 97 206 221
Fragments arranged by size:
221 206 97 76
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 46 78 600
Size: 46 32 522
Fragments arranged by size:
522 46 32
PstI C_TGCA'G
Cuts at: 0 103 600
Size: 103 497
PvuII CAG'CTG
Cuts at: 0 73 600
Size: 73 527
SanDI GG'GwC_CC
Cuts at: 0 240 600
Size: 240 360
SfcI C'TryA_G
Cuts at: 0 99 600
Size: 99 501
SpeI A'CTAG_T
Cuts at: 0 544 600
Size: 544 56
StyI C'CwwG_G
Cuts at: 0 383 600
Size: 383 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 600
Size: 55 545
TatI w'GTAC_w
Cuts at: 0 317 600
Size: 317 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 600
Size: 174 426
ZraI GAC'GTC
Cuts at: 0 555 600
Size: 555 45
Enzymes that do cut:
AatII AflIII AgeI AlfI ApaI ApaLI ApoI BanII
BbsI BcgI BglI Bme1580I BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstEII Bsu36I ClaI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII HincII MluI MslI
MspA1I NspI PpuMI PspOMI PsrI PstI PvuII SanDI
SfcI SpeI StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AloI AlwNI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI Hin4I HindIII
HpaI KasI KpnI MfeI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI