(Linear) (Six-base) MAPSORT of: KIEE0626.seq  Check: 1379  from: 1  to: 1116



KIEE0626.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AccI GT'mk_AC

Cuts at:      0     802    1116 
   Size:        802     314
 
AflIII A'CryG_T

Cuts at:      0     144    1116 
   Size:        144     972
 
AlwNI CAG_nnn'CTG

Cuts at:      0      35    1116 
   Size:         35    1081
 
ApoI r'AATT_y

Cuts at:      0      76     115    1054    1116 
   Size:         76      39     939      62

  Fragments arranged by size: 

                 939      76      62      39
 
AvrII C'CTAG_G

Cuts at:      0     504    1116 
   Size:        504     612
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     419     872     902    1116 
   Size:        419     453      30     214

  Fragments arranged by size: 

                 453     419     214      30
 
BclI T'GATC_A

Cuts at:      0     684    1116 
   Size:        684     432
 
BglI GCCn_nnn'nGGC

Cuts at:      0     821    1116 
   Size:        821     295
 
BlpI GC'TnA_GC

Cuts at:      0     973    1023    1116 
   Size:        973      50      93

  Fragments arranged by size: 

                 973      93      50
 
Bme1580I G_kGCm'C

Cuts at:      0      46    1116 
   Size:         46    1070
 
BmgBI CAC'GTC

Cuts at:      0     441    1116 
   Size:        441     675
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     970    1055    1116 
   Size:        513     457      85      61

  Fragments arranged by size: 

                 513     457      85      61
 
Bpu10I CC'TnA_GC

Cuts at:      0     265    1116 
   Size:        265     851
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     732    1116 
   Size:        732     384
 
BsaBI GATnn'nnATC

Cuts at:      0     230    1116 
   Size:        230     886
 
BsaWI w'CCGG_w

Cuts at:      0     793    1116 
   Size:        793     323
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1053    1083    1116 
   Size:       1053      30      33

  Fragments arranged by size: 

                1053      33      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136    1116 
   Size:        136     980
 
Bsp1286I G_dGCh'C

Cuts at:      0      46    1116 
   Size:         46    1070
 
BsrFI r'CCGG_y

Cuts at:      0      14     741    1116 
   Size:         14     727     375

  Fragments arranged by size: 

                 727     375      14
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      35    1116 
   Size:         35    1081
 
BstYI r'GATC_y

Cuts at:      0     155     572    1116 
   Size:        155     417     544

  Fragments arranged by size: 

                 544     417     155
 
BtgI C'CryG_G

Cuts at:      0     195    1116 
   Size:        195     921
 
BtsI GCAGTG_nn'

Cuts at:      0     259    1116 
   Size:        259     857
 
EaeI y'GGCC_r

Cuts at:      0     251    1116 
   Size:        251     865
 
EarI CTCTTCn'nnn_

Cuts at:      0      52     670    1116 
   Size:         52     618     446

  Fragments arranged by size: 

                 618     446      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     970    1055    1116 
   Size:        513     457      85      61

  Fragments arranged by size: 

                 513     457      85      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     500     532     669     701     759     791    1116 
   Size:        500      32     137      32      58      32     325

  Fragments arranged by size: 

                 500     325     137      58      32      32      32
 
HincII GTy'rAC

Cuts at:      0     168    1116 
   Size:        168     948
 
HindIII A'AGCT_T

Cuts at:      0     846     975    1116 
   Size:        846     129     141

  Fragments arranged by size: 

                 846     141     129
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     757    1116 
   Size:        757     359
 
MslI CAynn'nnrTG

Cuts at:      0     400     733    1116 
   Size:        400     333     383

  Fragments arranged by size: 

                 400     383     333
 
MspA1I CmG'CkG

Cuts at:      0      32    1116 
   Size:         32    1084
 
NcoI C'CATG_G

Cuts at:      0     195    1116 
   Size:        195     921
 
NspI r_CATG'y

Cuts at:      0     148    1069    1116 
   Size:        148     921      47

  Fragments arranged by size: 

                 921     148      47
 
PciI A'CATG_T

Cuts at:      0     144    1116 
   Size:        144     972
 
PfoI T'CCnGG_A

Cuts at:      0     518    1116 
   Size:        518     598
 
PshAI GACnn'nnGTC

Cuts at:      0     423    1116 
   Size:        423     693
 
SapI GCTCTTCn'nnn_

Cuts at:      0      52     670    1116 
   Size:         52     618     446

  Fragments arranged by size: 

                 618     446      52
 
SfcI C'TryA_G

Cuts at:      0     300    1116 
   Size:        300     816
 
StyI C'CwwG_G

Cuts at:      0     195     504    1116 
   Size:        195     309     612

  Fragments arranged by size: 

                 612     309     195
 
TatI w'GTAC_w

Cuts at:      0     613    1116 
   Size:        613     503
 
XmnI GAAnn'nnTTC

Cuts at:      0     563    1116 
   Size:        563     553
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI    AvrII     BbsI     BclI     BglI
     BlpI Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BsaBI    BsaWI
    BsaXI    BseRI Bsp1286I    BsrFI   BstAPI    BstYI     BtgI     BtsI
     EaeI     EarI  Eco57MI    Hin4I   HincII  HindIII     MmeI     MslI
   MspA1I     NcoI     NspI     PciI     PfoI    PshAI     SapI     SfcI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI