(Linear) (Six-base) MAPSORT of: KIEE0617.seq  Check: 2551  from: 1  to: 549



KIEE0617.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     441     549 
   Size:        441     108
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     212     549 
   Size:         89     123     337

  Fragments arranged by size: 

                 337     123      89
 
AflIII A'CryG_T

Cuts at:      0      26     549 
   Size:         26     523
 
AhdI GACnn_n'nnGTC

Cuts at:      0      63     549 
   Size:         63     486
 
ApoI r'AATT_y

Cuts at:      0      85     549 
   Size:         85     464
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     336     369     549 
   Size:        336      33     180

  Fragments arranged by size: 

                 336     180      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      77     200     459     549 
   Size:         77     123     259      90

  Fragments arranged by size: 

                 259     123      90      77
 
BmgBI CAC'GTC

Cuts at:      0      29     549 
   Size:         29     520
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     507     549 
   Size:        507      42
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     393     549 
   Size:        393     156
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      35     549 
   Size:         35     514
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      20     549 
   Size:         20     529
 
BsrGI T'GTAC_A

Cuts at:      0     311     549 
   Size:        311     238
 
BtgI C'CryG_G

Cuts at:      0     457     549 
   Size:        457      92
 
EaeI y'GGCC_r

Cuts at:      0      50     549 
   Size:         50     499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     212     549 
   Size:         89     123     337

  Fragments arranged by size: 

                 337     123      89
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     413     549 
   Size:        413     136
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     264     296     549 
   Size:         49      32     183      32     253

  Fragments arranged by size: 

                 253     183      49      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     549 
   Size:        503      46
 
MspA1I CmG'CkG

Cuts at:      0     503     549 
   Size:        503      46
 
PasI CC'CwG_GG

Cuts at:      0     258     549 
   Size:        258     291
 
PstI C_TGCA'G

Cuts at:      0     502     549 
   Size:        502      47
 
PvuII CAG'CTG

Cuts at:      0     503     549 
   Size:        503      46
 
SexAI A'CCwGG_T

Cuts at:      0      61     549 
   Size:         61     488
 
SfcI C'TryA_G

Cuts at:      0     104     411     498     549 
   Size:        104     307      87      51

  Fragments arranged by size: 

                 307     104      87      51
 
StyI C'CwwG_G

Cuts at:      0     293     549 
   Size:        293     256
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     383     459     549 
   Size:        383      76      90

  Fragments arranged by size: 

                 383      90      76
 
TatI w'GTAC_w

Cuts at:      0     311     515     549 
   Size:        311     204      34

  Fragments arranged by size: 

                 311     204      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     129     161     205     237     549 
   Size:        129      32      44      32     312

  Fragments arranged by size: 

                 312     129      44      32      32
 
XbaI T'CTAG_A

Cuts at:      0     355     549 
   Size:        355     194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     500     549 
   Size:        500      49
 
XmnI GAAnn'nnTTC

Cuts at:      0     474     549 
   Size:        474      75
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     ApoI     BaeI     BbsI    BmgBI
     BmrI    BseRI    BsmBI    BspMI    BsrGI     BtgI     EaeI  Eco57MI
     FalI    Hin4I     MmeI   MspA1I     PasI     PstI    PvuII    SexAI
     SfcI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XhoI     XmaI     ZraI