Restriction map for
pF1KE0616
(Linear) (Six-base) MAPSORT of: KIEE0616.seq Check: 3596 from: 1 to: 948
KIEE0616.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
Acc65I G'GTAC_C
Cuts at: 0 346 948
Size: 346 602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 299 597 948
Size: 27 272 298 351
Fragments arranged by size:
351 298 272 27
AfeI AGC'GCT
Cuts at: 0 420 948
Size: 420 528
AflIII A'CryG_T
Cuts at: 0 551 948
Size: 551 397
ApaI G_GGCC'C
Cuts at: 0 28 369 948
Size: 28 341 579
Fragments arranged by size:
579 341 28
ApoI r'AATT_y
Cuts at: 0 331 948
Size: 331 617
AvaI C'yCGr_G
Cuts at: 0 21 541 948
Size: 21 520 407
Fragments arranged by size:
520 407 21
BanI G'GyrC_C
Cuts at: 0 346 534 794 948
Size: 346 188 260 154
Fragments arranged by size:
346 260 188 154
BanII G_rGCy'C
Cuts at: 0 28 369 446 524 587 610 684 897
Size: 28 341 77 78 63 23 74 213
Cuts at: 897 948
Size: 51
Fragments arranged by size:
341 213 78 77 74 63 51 28
23
BciVI GTATCCnnnnn_n'
Cuts at: 0 947 948
Size: 947 1
BglI GCCn_nnn'nGGC
Cuts at: 0 441 605 948
Size: 441 164 343
Fragments arranged by size:
441 343 164
Bme1580I G_kGCm'C
Cuts at: 0 28 369 539 720 948
Size: 28 341 170 181 228
Fragments arranged by size:
341 228 181 170 28
BmgBI CAC'GTC
Cuts at: 0 552 948
Size: 552 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 607 948
Size: 155 452 341
Fragments arranged by size:
452 341 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 948
Size: 820 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 269 499 948
Size: 269 230 449
Fragments arranged by size:
449 269 230
BsaWI w'CCGG_w
Cuts at: 0 557 684 948
Size: 557 127 264
Fragments arranged by size:
557 264 127
BseYI C'CCAG_C
Cuts at: 0 368 948
Size: 368 580
BsiHKAI G_wGCw'C
Cuts at: 0 623 684 948
Size: 623 61 264
Fragments arranged by size:
623 264 61
Bsp1286I G_dGCh'C
Cuts at: 0 28 369 446 524 539 587 610 623
Size: 28 341 77 78 15 48 23 13
Cuts at: 623 684 720 897 948
Size: 61 36 177 51
Fragments arranged by size:
341 177 78 77 61 51 48 36
28 23 15 13
BsrBI CCG'CTC
Cuts at: 0 732 948
Size: 732 216
BsrFI r'CCGG_y
Cuts at: 0 791 948
Size: 791 157
BtgI C'CryG_G
Cuts at: 0 699 948
Size: 699 249
EcoICRI GAG'CTC
Cuts at: 0 682 948
Size: 682 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 155 299 597 607 948
Size: 27 128 144 298 10 341
Fragments arranged by size:
341 298 144 128 27 10
EcoO109I rG'GnC_Cy
Cuts at: 0 25 365 948
Size: 25 340 583
Fragments arranged by size:
583 340 25
EcoRI G'AATT_C
Cuts at: 0 331 948
Size: 331 617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 490 867 948
Size: 490 377 81
Fragments arranged by size:
490 377 81
FspI TGC'GCA
Cuts at: 0 194 948
Size: 194 754
HaeII r_GCGC'y
Cuts at: 0 422 948
Size: 422 526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 628 660 948
Size: 128 32 468 32 288
Fragments arranged by size:
468 288 128 32 32
KpnI G_GTAC'C
Cuts at: 0 350 948
Size: 350 598
MfeI C'AATT_G
Cuts at: 0 437 948
Size: 437 511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 948
Size: 527 421
MslI CAynn'nnrTG
Cuts at: 0 188 948
Size: 188 760
MspA1I CmG'CkG
Cuts at: 0 118 225 385 486 948
Size: 118 107 160 101 462
Fragments arranged by size:
462 160 118 107 101
NcoI C'CATG_G
Cuts at: 0 699 948
Size: 699 249
PasI CC'CwG_GG
Cuts at: 0 35 948
Size: 35 913
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 630 948
Size: 78 552 318
Fragments arranged by size:
552 318 78
PfoI T'CCnGG_A
Cuts at: 0 132 948
Size: 132 816
PshAI GACnn'nnGTC
Cuts at: 0 694 948
Size: 694 254
PspOMI G'GGCC_C
Cuts at: 0 24 365 948
Size: 24 341 583
Fragments arranged by size:
583 341 24
PvuII CAG'CTG
Cuts at: 0 118 225 385 486 948
Size: 118 107 160 101 462
Fragments arranged by size:
462 160 118 107 101
SacI G_AGCT'C
Cuts at: 0 684 948
Size: 684 264
SmaI CCC'GGG
Cuts at: 0 23 948
Size: 23 925
SmlI C'TyrA_G
Cuts at: 0 835 948
Size: 835 113
StyI C'CwwG_G
Cuts at: 0 699 948
Size: 699 249
TatI w'GTAC_w
Cuts at: 0 872 948
Size: 872 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 921 948
Size: 921 27
Tth111I GACn'n_nGTC
Cuts at: 0 171 925 948
Size: 171 754 23
Fragments arranged by size:
754 171 23
XmaI C'CCGG_G
Cuts at: 0 21 948
Size: 21 927
Enzymes that do cut:
Acc65I AcuI AfeI AflIII ApaI ApoI AvaI BanI
BanII BciVI BglI Bme1580I BmgBI BpmI BpuEI BsaI
BsaWI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BtgI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I KpnI
MfeI MmeI MslI MspA1I NcoI PasI PflMI PfoI
PshAI PspOMI PvuII SacI SmaI SmlI StyI TatI
TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoRV FseI FspAI HincII HindIII HpaI
KasI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XcmI XhoI XmnI
ZraI