(Linear) (Six-base) MAPSORT of: KIEE0613.seq  Check: 9968  from: 1  to: 516



KIEE0613.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     499     516 
   Size:        499      17
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     516 
   Size:        299     217
 
AflII C'TTAA_G

Cuts at:      0     369     516 
   Size:        369     147
 
AgeI A'CCGG_T

Cuts at:      0     486     516 
   Size:        486      30
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303     516 
   Size:        303     213
 
ApoI r'AATT_y

Cuts at:      0      34     386     516 
   Size:         34     352     130

  Fragments arranged by size: 

                 352     130      34
 
BanII G_rGCy'C

Cuts at:      0     221     516 
   Size:        221     295
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     247     257     287     516 
   Size:        247      10      30     229

  Fragments arranged by size: 

                 247     229      30      10
 
BclI T'GATC_A

Cuts at:      0     191     516 
   Size:        191     325
 
BlpI GC'TnA_GC

Cuts at:      0      71     516 
   Size:         71     445
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     390     516 
   Size:        390     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     223     516 
   Size:         26     197     293

  Fragments arranged by size: 

                 293     197      26
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     516 
   Size:        376     140
 
BsaWI w'CCGG_w

Cuts at:      0     486     516 
   Size:        486      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     516 
   Size:        106      30     380

  Fragments arranged by size: 

                 380     106      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     516 
   Size:         61     455
 
BseYI C'CCAG_C

Cuts at:      0      66     516 
   Size:         66     450
 
BsmI GAATG_Cn'

Cuts at:      0      13      61     516 
   Size:         13      48     455

  Fragments arranged by size: 

                 455      48      13
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     516 
   Size:        221     295
 
BsrFI r'CCGG_y

Cuts at:      0     486     516 
   Size:        486      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     303     516 
   Size:        303     213
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      50     516 
   Size:         50     466
 
Bsu36I CC'TnA_GG

Cuts at:      0     445     516 
   Size:        445      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     223     299     516 
   Size:         26     197      76     217

  Fragments arranged by size: 

                 217     197      76      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     130     516 
   Size:        130     386
 
EcoO109I rG'GnC_Cy

Cuts at:      0     300     516 
   Size:        300     216
 
EcoRI G'AATT_C

Cuts at:      0      34     516 
   Size:         34     482
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     421     516 
   Size:        421      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     183     215     516 
   Size:        183      32     301

  Fragments arranged by size: 

                 301     183      32
 
HincII GTy'rAC

Cuts at:      0     500     516 
   Size:        500      16
 
MspA1I CmG'CkG

Cuts at:      0      75     516 
   Size:         75     441
 
NspI r_CATG'y

Cuts at:      0     472     516 
   Size:        472      44
 
SalI G'TCGA_C

Cuts at:      0     498     516 
   Size:        498      18
 
SfcI C'TryA_G

Cuts at:      0     108     516 
   Size:        108     408
 
SmlI C'TyrA_G

Cuts at:      0     355     369     516 
   Size:        355      14     147

  Fragments arranged by size: 

                 355     147      14
 
StuI AGG'CCT

Cuts at:      0     450     516 
   Size:        450      66
 
StyI C'CwwG_G

Cuts at:      0     126     516 
   Size:        126     390
 
XbaI T'CTAG_A

Cuts at:      0      30     516 
   Size:         30     486
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AgeI    AlwNI     ApoI    BanII     BbsI
     BclI     BlpI     BmrI     BpmI    BpuEI    BsaWI    BsaXI    BseRI
    BseYI     BsmI Bsp1286I    BsrFI   BstAPI    BstXI   Bsu36I  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII   MspA1I     NspI
     SalI     SfcI     SmlI     StuI     StyI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI